Replicated wild type berlin flies in the flight simulator

Comparison between White 1 ( Control (NorpA- UAS Chrimson)) and Yellow 1 (Positive Control ( Gr28bd-G4, TrpA1-G4).

To test whether the flies are really blind, and there is no problem with the NorpA part of the construct, we compare with NorpA,UAS GTACR1 ; NorpA,UAS GTACR2 ; and another stock of NorpA-UAS-Chrimson.

Weighted mean is calculated by multiplying the weights (total number of flies in that experiment/total number of flies in all the experiments) with the PI for that experiment, and taking the sum thereafter for all the experiments.

the first graph shows Bar plot of Mean and standard deviation of PIs for Genetic control (n=9) and Positive control (n=5).
The second graph shows weighted mean and weighted standard deviation of the same.

Weighted mean is calculated by multiplying the weights (total number of flies in that experiment/total number of flies in all the experiments) with the PI for that experiment, and taking the sum thereafter for all the experiments.

Positive control not working: why?

The results from wiltype Berlin flies in the flight simulator.

This is a picture of the supplemental figure from Maye et al. 2007

Below the results from the sathish scripts running on the data from Maye et al. 2007. It matches, so Sathish scripts in my hands work fine

This is the technique we are using to create the non conditional constructs: HDR using CRISPR/Cas9 (S. J. Gratz et al., 2014)

and these are the schemes of the constructs we are creating

Confocal image MAX stack of one of the brains at 20x

and at 40x

In this link we have a video of a 3D stainning pattern          zoomed_CC

In addition I add here teh boxplots from the final results of the Ping Pong ball setup with these experiments