qPCR-Joel

on Wednesday, June 3rd, 2020 1:43 | by

I did the graph on Joel’s data
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Pooling data not possible: working on a fix

on Tuesday, June 2nd, 2020 12:25 | by

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Last week

on Monday, June 1st, 2020 10:56 | by

  1. Made crosses for pupae TS
  2. Made cross for Tdc2-Gal4 and FoxP-LexA to see the VNC (I already had last week the pictures of the CB)
  3. Made crosses for ok107
  4. Made crosses for C380

After all those crosses will hatch I will have finished with the Buridan for now.

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Tub-Gal80 Adult increased N

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Did it again and now looks fine :)

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Dwelling time errors, prevents code from running

on Monday, May 25th, 2020 12:28 | by

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Statistical evaluation of OR

on | by

Recently I measured the optomotor response in T4/T5 flies. As expected, they did not respond to the optomotor stimulus as seen in the left chart below. However, statistical evaluation struggles to quantify this difference. It might be that this is because of the low sample size, or that we are using the wrong statistical analysis?

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FoxP-Tdc2

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no colocalization

Correction: the red is Tdc2, while the green is FoxP

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D42-increased N

on Monday, May 18th, 2020 11:24 | by

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Test of set-up after repair.

The signal is drifting again. Also adding a spacer between clamp and device is not solving it, but making the signal more noisy.

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FoxP locus + translations

on Wednesday, May 13th, 2020 2:32 | by


We understood that due to a stop codon in the intron 7 sequence, the Isoform-IR can not be translated until the end. The FH domain in the Isoform-IR is not complete, due to the fact that the intron do not participate in it. It’s thus likely that the IR is not acting properly as a transcription factor since the DNA binding domain is not complete.
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