LexA+Gal4 larvae

on Friday, March 13th, 2020 3:30 | by

I tried the fast way but is not usable. Because without doing an additional crossing I have only GFP and RFP in heterozygosis. which gives a very weak signal. Furthermore I have to remove (flox) the dsRED that is there from the LexA insertion. Otherwise in the larva I see some cells that are red but that are due to the dsRED and not to the RFP. Which means other crossings. In total, to have this picture I need 5 crosses.
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Category: Foxp | 2 Comments

Complete Buridan

on Tuesday, March 3rd, 2020 5:44 | by

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Category: buridan, Foxp | No Comments

UASCas9;UASgFoxP X ElavG4;TubGal80

on Monday, March 2nd, 2020 1:33 | by

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Comparison FoxP and FoxP/+

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Supplementary figure S1 and S2

on Thursday, February 27th, 2020 11:13 | by

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Category: Foxp | No Comments

Waiting for crosses to hatch

on Wednesday, February 26th, 2020 1:46 | by

  1. ato-Gal4 x conditional KO —> Removing FoxP in Dorsal Cluster Neurons for Buridan
  2. cmpy-Gal4 x conditional KO —> Removing FoxP in Protocerebral Bridge for Buridan
  3. temperature switch at embryo stage but shorter time —> for replicating the KO phenotype
  4. LexA + Gal4 together to analyze larval brain (I have always considered larvae except for this experiment, so I am doing it to be more complete)

Furthermore, for not loosing time this week I did:

  1. Leipzig presentation
  2. Wrote a bit of results for paper/thesis
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Optomotor evaluation

on Wednesday, February 19th, 2020 1:51 | by

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Optogenetic T-maze

on | by

The figure below contains the separate data from both Emer, and Ferruh/Kader. Both data sets have been merged too.

Fly linesample size
Gr28 ATR60
Gr28no56
W111835
THD’-ATR35
THD’no36
THD1no35
THD1-ATR37
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Control cross

Control cross of UAS-Cas9;UAS-gFoxP with ELAV-GAL4;UAS-Cas9 is working. Flys are not able to fly and are walking poorly.

Control cross of Hs-Gal4 with UAS-Stinger is still not working.

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UASCas9;UASgFoxP X ElavG4;TubGal80

Update on coditional FoxP manipulation. Either no manipulation at 18°c or switch to 30°c for adult flys.

Top: switch to 30°c for adult flys.
Bottom: no switch in temperatur
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