FoxP-LexA – cell counting (IMARIS)

on Monday, July 22nd, 2019 10:38 | by

3rd instar larvae: 406 (n=2)

Adult: 1430 (n=2) (VNC excluded)

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FoxP-LexA + nc82

on Thursday, July 18th, 2019 10:45 | by

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FoxPB-Gal4+nc82

on Saturday, July 13th, 2019 3:28 | by

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FoxP-LexA x GFP Larvae

on Friday, July 12th, 2019 2:40 | by

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Tub-Gal80 flies with HS at embryo stage + statistic on old results

on Friday, July 5th, 2019 11:44 | by

The combination of heat and the FoxP KO is making the flies too sick to develop properly. This leads to difficulties in growing. Even though I can not test for behaviour it´s a good result because it means that the Tub-Gal80 line is working, and it validates my other results

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FoxP conditional KO – immunohistochemistry with FoxP antibody

on Friday, June 14th, 2019 10:54 | by

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Cell count on CB and VNC

on Friday, May 17th, 2019 12:00 | by

The previous cell count was done considering only the central brain. Here I included the VNC in order to have a more complete information

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ChAT on adults

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Unfortunately it looks like it is impossible for me to recognize cell bodies. I can only see neuropil, therefore I can not look for colocalizations.

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Optimizing optomotor response, part 2

on Friday, May 10th, 2019 4:20 | by

The optomotor stimulus consists of a striped arena with panoramic vertical stripes rotating horizontally around the vertical, or yaw axis. Depending on the spatial wavelenght (or spatial frequency, SF) the fly’s optomotor response (OR) is changing supposedly to reach a maximum between 20-40°
(with a pattern motion of 0° ) . As the panoramic stripes are rotating a tethered fly will actively steer in the direction of the motion. After its maximum the OR gradually decreases with an increasing spatial frequency to finally reach 0 around 9.6-6.3°. A tendency to negative/reversed OR has been observed as the SF reaches 6.3°.

This experiment has been shown before in 1976 by E. Buchner, Elementary movement detectors in an insect visual system. Bioi Cybern 24:85- 101, Figure 8 page 93. Attaching Figure 8 as a reference.

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Optimizing optomotor response

on Tuesday, May 7th, 2019 4:56 | by

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