17d flight simulator

on Monday, October 1st, 2018 2:58 | by

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FoxP flight simulator

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FoxP climbing assay

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17d-GFP

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Flight Performance data, Optomotor response

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Counting of FoxP-isoB-Gal4 positive cells

on Wednesday, September 26th, 2018 10:19 | by

Counting of FoxP-isoB-Gal4 positive cells in adult and larvae brains (no VNC). Five brains counted for each age

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Beta results from first test drive OptoMoto

on Monday, September 24th, 2018 1:56 | by

The different stages of data processing:

1. single fly dyplot with all data present

E11(normalF)

2. Plot of all the even / odd periods of one fly

E11(normalF)even_period_plot

E11(normalF)odd_period_plot

 

3. Plot of all even / odd Periods of one fly merged

E11(normalF)even_merged_plot

E11(normalF)odd_merged_plot

 

4. Plot of the even/odd periods from all tested flys

 

Another example (first fly tested):

E1(normalF)period_plot

even_merged

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Tries with IMARIS

on Friday, September 21st, 2018 2:07 | by

Me and Anders spent some time at the imaris trying to get confidence with the software. We managed to do volume rendering and we counted the FoxP-isoB positive cells

  

the count resulted really similar to the one I´ve done by eyes:

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Protocerebral Bridge in FoxP-Gal4 and in FoxP-KO

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Datahandling OptoMotorics

on Monday, September 17th, 2018 12:31 | by

Table with the generated fly test data:

Test Description Average right Average left Differece general
Ex4 normal 4.55382 4.624879 0.071059 4.5893495
Ex4 repositioned 4.562121 4.579682 0.017561 4.5709015
Ex5 normal 4.273299 4.434383 0.161084 4.353841
Ex5 repositioned 4.562121 4.579682 0.1610834 4.5709015
Ex6 normal 3.535669 3.587334 0.051665 3.5615015
Ex7 no arena 3.349097 3.337588 -0.0115097 3.343343
Ex8 bad fly 3.42903 3.394603 -0.034427 3.411816
Ex9 no arena 4.391139 4.363279 -0.0278599 4.377209
Ex9 normal 4.404213 4.456195 0.05198185 4.430204
Ex10 no arena 4.259827 4.195971 -0.0638559 4.227899
Ex10 normal 4.243121 4.360124 0.1170035 4.301622

 

Ex8:

 

Ex9:

 

Ex10:

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