It seems that there is something more there. There were 5 batches of 3 flies per side reinforced. I put the intensity higher than ever before. I will try to get the maximum intensity for the next experiment and see what it looks like.

Pilot experiment(N=20)

Diazepam Experiment(N=10)

Rover, N=3

sitter, N=2

wtb, N=4

longterm, N=13 (old WTB excluded)

R,S self learning traces, and longterm memory trace

WTB self learning traces, longterm memory trace

I have decreased the light intensity to around half of what it previously was. The result do not show any clear phenotype. The ‘logical PI’ looks kind of inverted because I actually have plotted it so that positive means light, negative means no light. A total of 30 flies were tested: some of them with right, other with left reinforcement

Here is an update on the evaluation of the DA clusters in Photopreference.

The behavior of new and old WTB seems different, but since only a few flies are tested, the results are not conclusive.

newWTB =3, PI= 0.35

old WTB from last month = 7,  PI=0.04

flying trace,

wtb72 (old, left punishment)

wtb 74,78 (new, right punishment)

I want to continue on the Diazepam project and look if Serotonergic or Gabaergic circuits are involved in modulating photo-preference in flies. To begin with, I tried to replicate Lena’s experiments involving the individual T-maze. Each fly was introduced at the decision point of the T maze and once the fly moves towards either the light or the dark, it was pulled out of the T-maze using suction and reintroduced to the decision point (Each fly was tested ten times). In the first few days, I tried to standardise the apparatus and the light intensity. The results below indicate the choice index of normal and clipped WTB flies.

R=30

S=20

longterm memory N= 22

control N= 6

I am still testing different GAL4s in order to find out the dopamine clusters involved in photopreference shift. Here are some new results.