on Monday, November 2nd, 2020 11:17 | by Simon Benmaamar
I have tested 514 larvae from the forR/forS Control Group and 480 larvae from the forR/forS Crowded Group. Here are the results:
on Monday, October 26th, 2020 1:56 | by Andreas Ehweiner
Recent wtb test
There often seems to be a strong preference for a side. The laser was off.
Flies often seem to have a strong preferenc for a side. In the imiage below the top fly was punished for left torque and survived, the fly below was punished for the right torque side and died.
Trails with drift
on Monday, October 12th, 2020 12:49 | by Andreas Ehweiner
I’ve started again to test the set-up after repairs. It does not seem to be perfectly stable ,with some drift in the positive direction. Most often, it seems that the amount of drifting would be fine for measuring.
Therefor I tried measuring some flies. A problem now is a very negative baseline, when the fly stops flying. Also the response for left and right seems not to be equal.
So now I’m trying to get the line close to 0, so that I have conclusive results if the set-up if usable at the current state.
2 examples of drift:
on Monday, September 28th, 2020 12:21 | by Andreas Ehweiner
I retried the gene-switch method with a new solution of RU486. Since it did not worked before I just tried one line. The flies were left for two days on instant food mixed with a sugar solution of RU486. This time I saw normal GFP expression. So I will test all 4 line in parallel after the break.
on | by Andreas Ehweiner
I finished my cross for the colocalisation of FoxP with the 6 Gal4-lines i ordered.
Two lines show a nice colocalisation and will be tested.
One would show some overlap, but a completly diffrent expression pattern than it should have. So i will try this line again from the stock to exclude a mixup of the line.
The next one dont seem to have any overlap, but also the pattern looks not like it should.
This line seems to have coexpression but the pattern also looks a bit odd, this may be due to a general weak signal and a bad dissection.
The final line seems to have no overlap with the FoxP expression.
on Monday, September 14th, 2020 1:49 | by Andreas Ehweiner
The repetition of the knockout experiment in adult flies indicates that FoxP is not required for learning in adult flies. (Exp = elav tubGal80>Cas9gFoxP)
on | by Andreas Ehweiner
Comparison between Control (elavGal4 x CD8GFP) and hsGla4 x CD8GFP. Hs for 3h, fixation and dissection after 24h.
on Monday, August 31st, 2020 12:56 | by Anders Eriksson