self learning from WTB

on Friday, March 10th, 2017 2:30 | by

By contrast of PKG rover, this result shows PI from wtb. N = 4

test1 PI= -0.05

test2 PI= -0.08

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PKG rover update

on Monday, March 6th, 2017 10:17 | by

There is the result of 20 measurements on PKG rover.

pretest:-0.45

train1:0.56

train2:0.71

test1:0.47

train3:0.83

train4:0.87

test2:0.39

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Locomotion when light is on vs off

on Monday, February 27th, 2017 12:43 | by

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PKG rover update

on Friday, February 24th, 2017 1:48 | by

After adjusting the laser angle, PI got much improved. But, the test 2 phase shows a lower PI than test 1 in most of cases, the reason for that is still unclear. The following is the result of 9 measurements without drifting.

train1 PI: 0.50

train2: 0.42

test1: 0.39

train3:0.61

train4: 0.63

test2: 0.06 ???

 

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Yoked experiments analysis

on Monday, February 20th, 2017 2:46 | by

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Laser power/angle adjustment

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After camera and punish laser adjusted, flies showed a high avoidence in the trail. Especially, when the fly was hit by punish laser in the first time of each training block. Unfortunately, there was a huge drift in the last trace after adjustment, but it shows a good avoidence in each training block.

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Yoked experiments analysis

on Monday, February 13th, 2017 2:47 | by

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PKG rover from last week

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Generally, PKG drosophila were flying much better when they hatched from normal temperature. There is the result from 10 PKG drosophila.

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Drosophila behavioural flexibility towards food (3)

on Saturday, February 11th, 2017 10:21 | by

This time I tried to induce a kind of Garcia effect (eating something, feeling sick and then avoiding eating that again) in Drosophila. As one can see in the video below, Drosophila show inhibited proboscis extension response (PER) right after the onset of the US (intestinal discomfort).

 

However, the conditioned response is extinguished after several hours. I tried to use groups of 20 starved flies on yeast+water for 2h, food + 0.1% hydrogen peroxyde for 1h, starving with nothing for 1h (always inside vials), and then testing with conditioned food against another food for 2h (inside Petri dishes). It didn’t work: in the end they show the same preference as controls (without hydrogen peroxyde). I tried to condition sucrose, glycine, pineapple juice (and then test against apple juice) and nothing, the flies avoid at first but once the swelling goes away, they forget or don’t associate anything bad or sufficiently bad with that food anymore, and continue to prefer it.

I also confirmed the PER suppression following conditioning with quinine reported in [C. Keene A, Masek P. Optogenetic Induction of Aversive Taste Memory. Neuroscience. 2012;222:173-180]. However, it is also extinguished minutes (~30) after the conditioning, as their results also show (in my case, I used sucrose instead of fructose):

(my results:)

(their results:)

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Update on PKG sitter/rover PI

on Monday, February 6th, 2017 9:41 | by

There is a short update on summerized previous PI. Still, PKG transgenic drosophila is hardly testable, and the results seems not so promising. I’m going to focus on testing WTB this week, to compare the difference of PI between WTB and PKG

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