Update on the Diazepam Experiment

on Thursday, June 1st, 2017 4:05 | by

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Optimizing the Joystick with Gr28bd+TrpA1>Chrimson

on Monday, May 29th, 2017 12:58 | by

It seems that there is something more there. There were 5 batches of 3 flies per side reinforced. I put the intensity higher than ever before. I will try to get the maximum intensity for the next experiment and see what it looks like.

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Diazepam Experiment

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Pilot experiment(N=20)

 

Diazepam Experiment(N=10)

 

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Updates on R,S,WTB tests

on Friday, May 26th, 2017 1:20 | by

Rover, N=3

sitter, N=2

wtb, N=4

longterm, N=13 (old WTB excluded)

R,S self learning traces, and longterm memory trace

WTB self learning traces, longterm memory trace

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Looking for the phenotype in Gr28bd+TrpA1>Chrimson

on Monday, May 22nd, 2017 3:01 | by

I have decreased the light intensity to around half of what it previously was. The result do not show any clear phenotype. The ‘logical PI’ looks kind of inverted because I actually have plotted it so that positive means light, negative means no light. A total of 30 flies were tested: some of them with right, other with left reinforcement

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DA clusters and Photopreference

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Here is an update on the evaluation of the DA clusters in Photopreference.

 

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newWTB vs oldWTB

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The behavior of new and old WTB seems different, but since only a few flies are tested, the results are not conclusive.

newWTB =3, PI= 0.35

old WTB from last month = 7,  PI=0.04

flying trace,

wtb72 (old, left punishment)

wtb 74,78 (new, right punishment)

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Preliminary testing of the Individual T maze

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I want to continue on the Diazepam project and look if Serotonergic or Gabaergic circuits are involved in modulating photo-preference in flies. To begin with, I tried to replicate Lena’s experiments involving the individual T-maze. Each fly was introduced at the decision point of the T maze and once the fly moves towards either the light or the dark, it was pulled out of the T-maze using suction and reintroduced to the decision point (Each fly was tested ten times). In the first few days, I tried to standardise the apparatus and the light intensity. The results below indicate the choice index of normal and clipped WTB flies.

 

 

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update on rover&sitter test and longterm memory test

on Friday, May 12th, 2017 3:41 | by

R=30

S=20

longterm memory N= 22

control N= 6

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Update on DA & photopreference

on Monday, April 24th, 2017 1:55 | by

I am still testing different GAL4s in order to find out the dopamine clusters involved in photopreference shift. Here are some new results.

 

 

 

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