on Sunday, December 2nd, 2018 3:29 | by Ottavia Palazzo
The reaction is not optimal, but it looks like there are some double positive cells, and this indicate that some neurons could be motorneurons. To identify a % more and better reactions should be done
on | by Ottavia Palazzo
on Monday, November 26th, 2018 1:11 | by Max von der Linde
This post shows the latest day of testing and its results.
All with small hooks:
All with big hooks:
All on front of plarform:
All on back of platform:
-> big hooks and front of platform seem like promising factors, let’s pool them!
Front and big hooks:
Front and small hooks (worse):
last problem: If compared to manual stimulation, opposite signals are found. Wrong manual stimulation?
on Sunday, November 25th, 2018 3:46 | by Ottavia Palazzo
FoxP-KO: the homozigous FoxP-KO (with NO Gal4 and NO GFP) had a green signal, it means that the dsRed gives problems. I thus removed it. Also, in really nice dissections, I don´t see the phenotype I was seeing before. The brain looks fairly normal. it could than be that i wasn´t disecting well enough
on Monday, November 12th, 2018 2:14 | by Max von der Linde
The following Link shows the results of the latest tests with Wildtype flys, evaluated with the latest version of the HTML_DTS_project.R programm.
The histograms unfortunately show very little difference between the state of left turning arena and right turning arena.
on Monday, October 29th, 2018 2:38 | by Anders Eriksson
on Wednesday, October 24th, 2018 5:06 | by Ottavia Palazzo
This picture shows all of the positive clones in the ELISA from the 1st screen
While this picture shows just the clones that have a high response for the peptide the mice where injected with, a low response for any other random peptide, and furthermore a low response for BSA
on | by Ottavia Palazzo
I add another animal to the count (so now n=5) and the standard deviation increased. Since I used a very young fly it could be that the cell number is age dependent. But since it can be also individual differences, after San Diego I will make an age related analysis
on Monday, October 22nd, 2018 10:10 | by Max von der Linde
on Wednesday, October 17th, 2018 4:04 | by Ottavia Palazzo
Here´s a picture of a WTB fly and a foxP-KO-homozigous. I dissected all together and I did not know which one was which. But I manage to recognize them, which means that they are well recognizable. Just to be sure i will do that another time. (Also in order to have better dissections).
The distance between the two “hemispheres” in the WTB has an average of 5,4 um (n=3)
The distance between the two “hemispheres” in the KO has an average of 32,2 um (n=2)
The length of the protocerebral bridge in the WTB has an average of 95,7 um (n=3)
The the protocerebral bridge in the KO has an average of 140,3 um (n=2)