More data from the basement
on Monday, July 8th, 2024 8:15 | by Ellie
With the modified laser settings I was able to test 3 more wtb. Here are the results:
![](https://lab.brembs.net/wp/wp-content/uploads/2024/07/new-data-1024x468.png)
–> yaw torque
Testing the basement flight simulator
on Wednesday, June 19th, 2024 3:46 | by Ellie
Before collecting the actual data I make sure that the flight simulator in the basement does its thing ;) Here are the test runs with wtb flies for different protocols:
![](https://lab.brembs.net/wp/wp-content/uploads/2024/06/yaw-torque-1024x471.png)
—>> yaw torque
![](https://lab.brembs.net/wp/wp-content/uploads/2024/06/switch-mode-1-1024x467.png)
–>> switch mode
![](https://lab.brembs.net/wp/wp-content/uploads/2024/06/habit-formation-1024x467.png)
–>> habit formation
Bachelor Blog / #7 offspring
on Monday, October 2nd, 2023 10:51 | by Ellie
Below you can find the data I collected from the offspring flies:
![](https://lab.brembs.net/wp/wp-content/uploads/2023/10/parents_trained-1024x394.png)
-> offspring from trained parents
![](https://lab.brembs.net/wp/wp-content/uploads/2023/10/parents_untrained-1-1024x394.png)
-> offspring from untrained parents
(I left out data from flies that showed negative preference during two training periods in a row)
Bachelor Blog / #6 playing around
on Monday, September 18th, 2023 12:51 | by Ellie
Since the results I got after training the parental flies looked a bit odd on first sight I decided to take a closer view…
First I excluded some weird animals that either showed a larger preference for one side than avoidance or showed no avoidance two training periods in a row:
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/Screenshot-2023-09-18-121413-1-1024x573.png)
Next I compared the behavior of flies that showed avoidance but no learning with the behavior of the remaining flies:
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/Screenshot-2023-09-18-121512-1-1024x573.png)
-> Avoidance is almost the same but note the first test period!
Lastly I split the data according to male and female flies. Here is what I got:
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/Screenshot-2023-09-18-121527-1-1024x575.png)
-> Looks a bit like there is negative learning in the male flies however I don´t have enough data to be sure…
As an overview here are all the flies (except for the excluded ones) together again:
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/all-flies-1024x393.png)
Bachelor Blog / #4 is there something?
on Monday, August 7th, 2023 2:13 | by Ellie
The offspring of my first experimental fly cohort finally hatched! Below you find a few first pre-tests I ran last week :)
First, here are the results of a quick test to see if the offspring shows a preference for the parentally trained side after the first training period:
![](https://lab.brembs.net/wp/wp-content/uploads/2023/08/offs_ExpA-1024x589.png)
After that I played around with the laser a little bit to find the learning threshhold. I set the laser on 2,6V but the results I got look a bit weird:
![](https://lab.brembs.net/wp/wp-content/uploads/2023/08/TH_ExpA-1024x461.png)
-> untrained wtb flies
![](https://lab.brembs.net/wp/wp-content/uploads/2023/08/TH_offs_ExpA-1024x460.png)
-> offspring of trained wtb flies
I´m optimistic however there is still a lot of work to do…
Bachelor Blog / #1 outline
on Monday, May 22nd, 2023 8:45 | by Ellie
Hello everyone!
Here is a brief overview of the topics I will be dealing with over the next few months:
![](https://lab.brembs.net/wp/wp-content/uploads/2023/05/outline-1024x576.png)
This is the experiment by MD Ziv Williams I am trying to imitate ->
If you are further interested in transgenerational inheritance of behavioral traits and non synaptic memory storage check out these interesting links ->
- Can brain activity transmit transgenerationally? – Miska and Rechavi, 2021
- Is plasticity of synapses the mechanism of long-term memory storage? – Abraham, Jones and Glanzman, 2019
This week, for now, I will continue practicing to glue the flies and make a realistic estimate of the number of flies I need to get a significant result. Hopefully I can start trying out my experimental setup by the start of next week :)
Strokelitude – Final Work
on Monday, April 15th, 2019 2:41 | by Miroslav Stojic
Final Stats (flew more than 8min/out of):
– Wild type: 14/17 (82%)
– ElavGal4 x UAS-Ryr 28919: 3/13 (23%)
– ElavGal4 x UAS-Ryr 65885: 13/100 (13%)
Wild Types:
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-7-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-8-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-9-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-10-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-11-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-12-1024x256.png)
ElavGal4 x UASRyR 65885
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-13-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-14-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-15-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-16-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-17-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-18-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-19-1024x256.png)
Category: Spontaneous Behavior, strokelitude, WingStroke | No Comments
Strokelitude – New Findings and Recordings (1.-7. Apr.)
on Monday, April 8th, 2019 1:14 | by Miroslav Stojic
Last week (1.-7.Apr 2019) we noted how many flies want to fly more than 8 min and here is the result (only the ones we noted):
– Wild type: 8/9 (88%)
– ElavGal4 x UAS-Ryr 28919: 3/12 (25%)
– ElavGal4 x UAS-Ryr 65885: 6/35 (17%)
Trace (Downsampled 5) Graphs of Recordings
Wild type:
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-3-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-4-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-5-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-6-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-7-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-8-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-9-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-10-1024x256.png)
ElavGal4 x UAS-Ryr 28919:
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-11-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-12-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-13-1024x256.png)
ElavGal4 x UAS-Ryr 65885:
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-14-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-15-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-16-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-17-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-18-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-19-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-20-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-21-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/TraceDownsampled-22-1024x256.png)
Category: crosses, Spontaneous Behavior, strokelitude, WingStroke | No Comments
Strokelitude 5 New Rec
on Monday, April 1st, 2019 2:54 | by Miroslav Stojic
New Findings:
Elav-Gal4 x UAS-SERCA 44581 flies => Lethal before adulthood! (in all 5 vials so far)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-1-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-4-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-5-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/04/Trace-6-1024x256.png)
Category: crosses, Spontaneous Behavior, strokelitude, WingStroke | No Comments
Strok(e)litude – Practice
on Monday, March 25th, 2019 12:49 | by Miroslav Stojic
And the journey begins…
ElavGal4 x UASRyr65 “the wrong vials”
– > Graphs of the Trace.png (RightTrace – LeftTrace):
![](https://lab.brembs.net/wp/wp-content/uploads/2019/03/Trace-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/03/Trace-1-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/03/Trace-2-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/03/Trace-3-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/03/Trace-4-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/03/Trace-5-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/03/Trace-6-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/03/Trace-7-1024x256.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2019/03/Trace-8-1024x256.png)
Category: Spontaneous Behavior, strokelitude, WingStroke | No Comments