MBON screen progress

on Monday, July 27th, 2026 12:43 | by

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Miray – JoyStick Results from 0273-Gal4

on Monday, July 27th, 2026 10:49 | by

Yellow-Light (800 Lux), Target N = 50

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MBON screen progress 29.06.2026

on Monday, June 29th, 2026 9:50 | by

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TH Driver lines adult expression verification

on Friday, June 26th, 2026 11:23 | by

MB304B>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

Janelia Fly Light Split-GAL4 reference

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Larval salt avoidance behavior

on Friday, June 26th, 2026 11:12 | by

3rd instar larvae were tested for their avoidance behavior on 1.5% Agarose/1.5% Agarose+1.5M Salt-Plate. MB Kenyon cells were optogenetically activated via Channelrhodopsin2-XXL during the experiment. TH-Gal4 positive dopaminergic cells were permanently ablated.

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MBON screen progress 19.06.2026

on Monday, June 22nd, 2026 12:15 | by

MBON screen of MB399 for MBON02, MB050B for MBON15, MB027B for MBON17 all crossed with TnTe and WTB x TnTe for the negative control. Flies are tethered in torque meter and one turning domain is punished with a hot laser invisible to the fly. Each turning domain is also associated with an arena color. The lights are turned off at the last two periods. Wild type flies fail to learn to avoid the punished domain after the training periods in these conditions. A positive PI-score in the 12th period indicates that the fly learned.
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MBON Screen progress 01.06.2026

on Monday, June 1st, 2026 12:59 | by

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MBON screen progress 18.05.2026

on Monday, May 18th, 2026 12:32 | by

PI- values for world learning torque experiments. As usual the flies were tethered and subjected to adverse stimuli by laser dependent on torque domain, which also controlled arena color. Flies that show positive PI at the end indicate premature habit formation as WT flies don’t learn under these conditions in this time frame.
Same as above except the pretest preference has been subtraced
Current p-values for all the tested groups. Should be 25-30 flies at trial end.
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MBON screen progress 11.05.2026

on Monday, May 11th, 2026 12:25 | by

PI-values unsubtracted:

Results from premature habit formation torque measurements of four drosophila crosses: MB399B, MB050B & MB027B gal4 driver lines crossed with UAS-Tetanus toxin lines and Wild type Berlin crossed with UAS-TnT as control. Which line is which is still unknown as the screen is incomplete and researcher must remain blind. It appears two crosses strongly form habits prematurely, one doesn’t and one is still on the borderline.

PI-values subtracted:

Same results as above except with pretest bias subtracted.

p-values:

p-values for the different groups
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Salt (1.5 M) avoidance test with larvae under red and blue light (N=20)

on Sunday, May 3rd, 2026 5:46 | by

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