MBON screen progress 19.06.2026

on Monday, June 22nd, 2026 12:15 | by

MBON screen of MB399 for MBON02, MB050B for MBON15, MB027B for MBON17 all crossed with TnTe and WTB x TnTe for the negative control. Flies are tethered in torque meter and one turning domain is punished with a hot laser invisible to the fly. Each turning domain is also associated with an arena color. The lights are turned off at the last two periods. Wild type flies fail to learn to avoid the punished domain after the training periods in these conditions. A positive PI-score in the 12th period indicates that the fly learned.
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Summary lab work

on Tuesday, June 9th, 2026 12:59 | by

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MBON Screen progress 01.06.2026

on Monday, June 1st, 2026 12:59 | by

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MBON screen progress 26.05.2026

on Tuesday, May 26th, 2026 9:05 | by

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MBON screen progress 11.05.2026

on Monday, May 11th, 2026 12:25 | by

PI-values unsubtracted:

Results from premature habit formation torque measurements of four drosophila crosses: MB399B, MB050B & MB027B gal4 driver lines crossed with UAS-Tetanus toxin lines and Wild type Berlin crossed with UAS-TnT as control. Which line is which is still unknown as the screen is incomplete and researcher must remain blind. It appears two crosses strongly form habits prematurely, one doesn’t and one is still on the borderline.

PI-values subtracted:

Same results as above except with pretest bias subtracted.

p-values:

p-values for the different groups
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Long form habit formation pooled data 16-20th & 23-27th of March 2026

on Monday, March 30th, 2026 12:07 | by

Performance index values for long sequence habit formation trial
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Short habit formation trial 11.03.2026

on Monday, March 16th, 2026 10:47 | by

Short form habit formation trial, indicating operant learning is abolished/delayed in the presence of external associative stimuli.
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Color learning 13.03.2026

on Monday, March 16th, 2026 10:38 | by

Results from first attempt at the habit formation trial. Strong positive preference in the beginning as well as low number of successful flies obscures the results. Reason for the disturbed OR at the end is that the offset for one fly was accidentally shifted while attempting to restart flight with a brush.
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Torque measurements 21.02 & 22.02

on Monday, February 23rd, 2026 12:21 | by

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Torque measurements 12.02.2026

on Friday, February 13th, 2026 12:38 | by

Torque results 12.02.2026, learning is abolished and many of the flies died. Think improper feeding is to blame. Granulated sugar had become encrusted and possibly inaccessible to the flies, potentially resulting in starvation. In the future, I’ll resolve to use less sugar and to clean more frequently to prevent this kind of buildup.
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