First data from aPKC knock-out in FoxP neurons

on Monday, May 13th, 2024 8:49 | by

When Andi tested his aPKC CRISPR knock-outs, he used the Tang torque meter setup, where the OMRs aren’t recorded. So I’m attempting to replicate his results and compare OMRs between groups.

It looks like I need to tweak the graph in some way to make the density plots show up. Obviously, N is too low to say anything yet, but I need to work on the plot.

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Results for MB145B, SS01716 and SS02180

on Monday, April 8th, 2024 10:33 | by

All figures show the results for preference tests (salt/pure) conducted under red and blue light for different gal4-lines.

MB145B:

SS01716:

SS02180:

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Results for amon-RNAi and MB143B

on Monday, February 26th, 2024 12:17 | by

The first figure shows the results for the preference test (salt/pure) conducted with amon-RNAi only under red light.

For the next experiment I conducted the same preference test but with the split-gal4 line MB143B under red and blue light.

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Optomotor graph coded

on Wednesday, February 21st, 2024 10:15 | by

There had been some concerns about the optomotor display in the group evaluation sheets showing right-turning torque on the left side of the graph and vice versa. Also, the use of standard deviations seemed to blur differences between the experimental groups:

Because of these concerns, I have swapped the traces and used standard error of the means instead of standard deviations:

What do you think? Better or worse? Feedback very welcome!

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Results sNPF mutants

on Tuesday, February 20th, 2024 12:52 | by

The following picture shows the results for the conducted preference tests (pure/salt) for sNPF mutants. All Experiments were performed under red light.

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Final ‘Joystick’ test results

on Monday, January 29th, 2024 11:30 | by

Over the course, control and test lines were experimented with under ‘Joystick’. The test lines include 13:0273-Gal 4 line, SS56699 line, and TH-C-AD; TH-D-DBD line. The tests were done under red and yellow light conditions.

Red light conditions:

Yellow light conditions:

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Finally done: rut and rsh flies better at self-learning

on Monday, January 22nd, 2024 1:36 | by

At long last, I got all the flies together that we need for sufficient statistical power. As the preliminary data had indicated, WTB flies don’t learn with the short training, while rut and rsh flies do just fine.

However, this may be due to genetic background effects, so we need to check the CRISPR mutants.

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T-maze (dark-light condition) test results

on Monday, December 11th, 2023 2:40 | by

The flies without curly (straight wings) for the line NorpA;20xUAS-Chrimson were tested in T-maze in dark-light conditions. The tests were conducted on 23 November, 27th November, and 29th November 2023. 8 tests were done for each pink and green line; hence a total of 16 tests were conducted. A total of 273 flies and 233 flies were tested for pink line and green line respectively.

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Starting it back up

on Monday, December 11th, 2023 11:34 | by

Last week, the torque mete ran for two days and I managed to record a few radish flies:

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Rover vs. Sitter self learning after 4 minutes training

on Monday, October 16th, 2023 10:39 | by

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