CantonS first trial, gustatory preferences

on Monday, December 2nd, 2024 3:01 | by

Category: Larve | No Comments

Final aPKC KO in b1/b3 motor neurons results – for now!

on Monday, December 2nd, 2024 2:57 | by

As it seems the flies without aPKC in b1 or in b3 steering motor neurons seem to learn fine, I’ve decided to leave this dataset where it is:

But I will try and analyze their optomotor response in more detail, maybe these flies can dissociate between the spontaneous preference and the OMR plasticity?

Joystick results Yellow light, testing with and Without food

on Monday, November 25th, 2024 1:31 | by

The positive control was tested once without food and once with food to observe its effect on general behavior, particularly on learning behavior. NF = No Food / WF = With Food

Self-learning, 8 minutes training, elav-Gal4>UAS-for-RNAi

on Monday, November 25th, 2024 11:47 | by

It seems that panneuronal downregulation of the expression of the foraging gene impairs yow torque learning when flies were trained for 8 minutes.

b1/b3 aPKC KO flies still learning, OMR unaffected

on Friday, November 22nd, 2024 3:47 | by

Now with over 20 flies in each group, it becomes more and more apparent that both the flies without aPKC in either b1 or b3 steering motor neurons still learn just fine:

As with the aPKC knock-out in FoxP neurons, also here, the optomotor response seems normal as well:

Interesting is the scatter in the slope parameter for the control flies:

Getting there: knocking out aPKC in b1 or b3

on Friday, November 15th, 2024 4:02 | by

Slowly getting the sample size going. As of now, it seems aPKC is either not needed in steering motor neurons b1 and b3, or that knocking aPKC out in only one of them is not sufficient to have an effect on operant self-learning. Shown is the first 2min test period after 8min of training, all three groups seem to show learning, at least at this stage:

All Joystick Results Yellow

on Monday, November 11th, 2024 11:16 | by

All Joystick Results Red

on Monday, November 11th, 2024 11:14 | by

Joystick Results Yellow Light 29.10.24

on Tuesday, October 29th, 2024 2:19 | by

Joystick Results Red Light 29.10.24

on Tuesday, October 29th, 2024 2:14 | by