MBON screen progress 14.09.2026

on Monday, September 14th, 2026 1:07 | by

HSP70 after heat shock in western blot

on Monday, September 14th, 2026 12:50 | by

Expression of HSP70 (70 kDa) after heatshock (HS) or no heatshock (n.S.) in groups of only female, male and both genders (mixed). Fluorescence was measured at a wavelength of 700 nm (anti-mouse) and 800 nm (anti-rat).

Expression of HSP70 (70 kDa) after heatshock (HS) or no heatshock (n.S.) in groups of only female, male and both genders (mixed). Fluorescence was measured at a wavelength of 800 nm (anti-rat).

Expression of HSP70 (70 kDa) after heatshock (HS) or no heatshock (n.S.) in groups of only female, male and both genders (mixed). Fluorescence was measured at a wavelength of 700 nm (anti-mouse).

Connectivity of MBONs

on Monday, September 14th, 2026 10:33 | by

MBON02 with b1 and b3 motor neurons in maleCNS dataset

MBON02 with b1 and b3 motor neurons in BANC dataset

MBON02 with descending neurons in maleCNS dataset

MBON02 with ascending neurons in maleCNS dataset

DNp32 (purple) and b1 and b3 motor neurons

Ömer: 0273-Gal4 T-Maze (red/yellow)

on Monday, September 7th, 2026 12:58 | by

Yellow Light (1000 Lux):

Red Light (1600 Lux)

Miray: Project summary

on Monday, September 7th, 2026 12:55 | by

Left: Expression patterns reported (top) and reproduced (bottom) for 0273-Gal4

Right: JoyStick results for yellow (left panels; 800 Lux) and red (right panels; 500 Lux)

Verification of RFP expression in stocks 539 and 550

on Friday, September 4th, 2026 3:56 | by

After some failed attempts at a visualizing co-localization of FoxP and aPKC using a cross of stock 504 and 539/550, I grew suspicious of the RFP co-expression of FoxP. I visualized the CNS’ of both 539 and 550 in the confocal microscope. The image on the left is stock 550 and the image on the right 539. Stock 539 doesn’t seem to express any RFP but 550 looks fine so I’ll be repeating the cross using 550 when I get a chance. Rat anti-RFP primary antibody and 555 anti-rat secondary antibody were used as opposed to mouse anti-mcherry from previous experiment.

Category: Anatomy, Foxp | No Comments

Summary lab work

on Monday, August 10th, 2026 1:06 | by

Connectome Interpreter – Effective Connectivitiy

on Monday, August 10th, 2026 12:17 | by

Known Cell type from Yijie Yin’s List

DNp29DNp29 neuromodulatory (dNPF), stimulation mimics food-deprivation; abdomen groomingneuromodulatory
DNp32DNp32 neuromodulatoryneuromodulatoryneuropeptide: myosupressin
DNp62DNp62 neuromodulatory; hungerneuromodulatoryneuropeptide: SIFamide
DNpe048DNpe048 neuromodulatoryneuromodulatoryneuropeptide: CCAP

Experimental Procedure to check increase/decrease in cAMP levels

on Monday, August 10th, 2026 10:43 | by

Miray- JoyStick with 0273-Gal4 (Red Light 500 Lux)

on Monday, August 10th, 2026 10:28 | by

All test and training periods
Tests against zero – Pretest
Tests against zero – First training
Tests against zero – Last training
U-Test First against Last Training period