Torque Measurements 24-27.2.2026

on Monday, March 2nd, 2026 4:39 | by

Results from tethered flight in torque meter. Fly fixed to hook and flight torque measured while laser punishes either left or right turning. Higher PI score indicates longer stay in unpunished domain, orange colored periods are punished. Higher scores in period 9, 12 and 13 indicates learning. Fly genotype is Wild type Berlin, N=16.
Same as above, with PI adjusted to factor in initial domain preference.
A low P-score indicates that the result is statistically significant and the flies indeed learned.
Pie charts indicate efficiency of experiment success. Blue wedge represents flies that completed trial, other colors represent flies that failed. Efficiency still low, using laser at 100% duty cycle often causes flies to stop flying unless directed at exactly the right position on their antennae.

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2.Yaw torque

on Monday, March 2nd, 2026 1:08 | by

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1. aPKC_TurboID_line

on Monday, March 2nd, 2026 1:07 | by

Generation of TurboID-linker-aPKC construct

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First feeding essay and wiggle factor

on Monday, March 2nd, 2026 12:51 | by

On the left, there are the results of the experimental group: social isolation, with the usual R Skript. The right side shows the same results but with an adjusted “flat threshold” (for movement) of 0.4 instead of 0.9.

First Feeding essay for experimental group “crowding” and “sleep deprivation”:

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stressor: heat

on Monday, March 2nd, 2026 12:33 | by

stressor: heat (37°C; 6min)

POS_CTRL: control group

Gr28bd+TrpA1(heat): experimental group

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TH Driver lines larval expression verification

on Monday, March 2nd, 2026 12:28 | by

NP0047-Gal4/CyO>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

TH-D’-Gal4>UAS>>mCD8::GFP,UAS>>mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

TH-D1-Gal4>UAS>>mCD8::GFP,UAS>>mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

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Salt (1.5 M) avoidance tests in larvae under blue and red light (N = 12)

on Sunday, March 1st, 2026 4:56 | by

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WTB Test for Torquemeter February 19-20

on Friday, February 27th, 2026 5:26 | by

10 flies completed out of 36 flies.

Optomotor traces (right/left) at start of experiment

Optomotor traces (right/left) at end of experiment

Performance Index bar plot with SEM:

Performance Index bar plot – preference subtracted:

Performance Index box&dotplot without notches:

Statistical tests of single groups against zero

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Retro-Tango endogenous GFP Expression

on Friday, February 27th, 2026 5:20 | by

On the right: Endogenous GFP expression (without antibody) from retro tango flies (BDSC: 99661) crossed with GF-Gal4 (BDSC: 79602) driver line.

On the left: GFP expression in retro-tango x GF-Gal4 flies from retro-tango publication ( https://doi.org/10.7554/eLife.85041 )

Same image but in retro-tango signal channel:

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RU_486 induced mCD8-GFP

on Monday, February 23rd, 2026 12:56 | by

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