I tested more wtb, this time with the switch mode protocol. Here are the results:

With the modified laser settings I was able to test 3 more wtb. Here are the results:

–> yaw torque

Before collecting the actual data I make sure that the flight simulator in the basement does its thing ;) Here are the test runs with wtb flies for different protocols:

—>> yaw torque

–>> switch mode

–>> habit formation

Below you can find the data I collected from the offspring flies:

-> offspring from trained parents

-> offspring from untrained parents

(I left out data from flies that showed negative preference during two training periods in a row)

Since the results I got after training the parental flies looked a bit odd on first sight I decided to take a closer view…

First I excluded some weird animals that either showed a larger preference for one side than avoidance or showed no avoidance two training periods in a row:

Next I compared the behavior of flies that showed avoidance but no learning with the behavior of the remaining flies:

-> Avoidance is almost the same but note the first test period!

Lastly I split the data according to male and female flies. Here is what I got:

-> Looks a bit like there is negative learning in the male flies however I don´t have enough data to be sure…

As an overview here are all the flies (except for the excluded ones) together again: