Finished salt (1.5 M) avoidance tests in larvae (Dop2R) under red and blue light
on Thursday, March 26th, 2026 2:25 | by Christoph Kumpfmüller
ARL <-> ABL: 0.009
BRL <-> BBL: 0.053
Category: Anatomy, crosses, genetics, Larvae, Mushroom Body, Optogenetics | No Comments
TH Driver lines larval expression verification
on Monday, March 16th, 2026 10:58 | by Radostina Lyutova
TH-D’-Gal4>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
NP1568-Gal4>UAS-mCD8::GFP (adult brains); aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
Category: Anatomy, Biogenic Amines, DAN, Larvae, TH | No Comments
TH Driver lines larval expression verification
on Tuesday, March 10th, 2026 12:04 | by Radostina Lyutova
NP1568-Gal4/CyO>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
Category: Anatomy, Biogenic Amines, Larvae, TH | No Comments
TH Driver lines larval expression verification
on Monday, March 2nd, 2026 12:28 | by Radostina Lyutova
NP0047-Gal4/CyO>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
TH-D’-Gal4>UAS>>mCD8::GFP,UAS>>mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
TH-D1-Gal4>UAS>>mCD8::GFP,UAS>>mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
Category: Anatomy, Biogenic Amines, Larvae | No Comments
Retro-Tango endogenous GFP Expression
on Friday, February 27th, 2026 5:20 | by Ipek Subay
On the right: Endogenous GFP expression (without antibody) from retro tango flies (BDSC: 99661) crossed with GF-Gal4 (BDSC: 79602) driver line.
On the left: GFP expression in retro-tango x GF-Gal4 flies from retro-tango publication ( https://doi.org/10.7554/eLife.85041 )
Same image but in retro-tango signal channel:
Category: Anatomy, retro-tango | No Comments
Control Experiments for Driver Line Expression
on Monday, February 16th, 2026 12:36 | by Ipek Subay
Giant fiber Gal4 driver (BDSC 79602), green channel shows giant fiber neurons, magenta channel shows background staining with nc82:
Giant fiber neurons on Codex BANC v626 dataset:
b1 mn driver (SS40980, also targets iii1 mn, iii3 mn):
Janelia image for SS40980:
b3 mn driver (SS48311, also targets hg1 MN, XBI002, WBI009):
Janelia image for SS48311:
b3 mn driver (SS98650):
Janelia image for SS98650:
Category: Anatomy, Habit formation, Motor neurons | No Comments
Control Experiments for Driver Line Expression
on Monday, February 9th, 2026 12:44 | by Ipek Subay
SS45779 – Targeting b3 MN, iii3 MN, hg1 MN
Our line:
Janelia image:
IS69306 – b1 mn driver line
Janelia image:
Retro-Tango control crossed with GF-Gal4 (BDSC: 79602), presynaptic neurons in magenta, giant fiber neurons in cyan and background staining in gray
Retro-Tango x GF-Gal4 from Sorkac et. al (2023)
rut and rad expression in b1 and b3 motor neurons, I looked for cells express 6 genes (rut, rad, FoxP, VGlut, AD TF, DBD TF) at the same time above certain threshold:
| Driver Line | AD TF | DBD TF | Targeted cells | Cells express all 6 genes at more than 0 | Cells express all 6 genes at more than 1 fold | Cells express all 6 genes at more than 2 fol |
| SS40980 | CG12680 | Cep104 (CG10137) | b1 MN, iii1 MN, iii3 MN | 0 | 0 | 0 |
| SS45779 | Ubx | ab | b3 MN, iii3 MN, hg1 MN | 158 | 0 | 0 |
| SS47160 | Ubx | hry | b2 MN, b3 MN, iii4 MN, hg1 MN, hg3 MN | 1 | 0 | 0 |
| SS48311 | Ubx | NetB | b3 MN, hg1 MN, XBI002, WBI009 | 78 | 44 | 2 |
| SS49806 | ??? | tim | b3 MN, hg1 MN, HBI017 | – | – | – |
| SS52405 | Ubx | CG4168 | b3 MN, i2 MN, hg3 MN | 46 | 25 | 1 |
| IS69306 | CG14388 (CG31345) | Awh | b1 driver | 0 | 0 | 0 |
| SS98650 | ct | Ubx | b3 driver | 76 | 39 | 0 |
| IS38497 | beat-IIIb | bab1 | b1&b3 | 34 | 15 | 1 |
Category: Anatomy, Motor neurons, retro-tango | No Comments
aPKC/FoxP colocalization confocal images
on Friday, January 23rd, 2026 12:52 | by Fridrik Kjartansson
Staining is a bit faint and brain quality a bit sub-optimal and no punctae can be observed in the FoxP channel, decreasing the gain did not resolve this, blocking was done for 1 hour at RT. Perhaps increasing concentration of 1st degree antibodies, especially for RFP might improve the results.
Category: Anatomy, crosses, Foxp, PKC, PKC_localisation | No Comments
TH-C-AD; TH-D-DBD x UAS-CD8::GFP Stainings
on Sunday, January 18th, 2026 3:48 | by Daniel Döringer
Green: GFP
Magenta: BRP
Category: Anatomy, Optogenetics | No Comments
Endogeneous GFP
on Monday, December 22nd, 2025 11:34 | by Julia Schulz
Category: Anatomy, crosses, genetics | No Comments