MBON screen progress 19.06.2026

on Monday, June 22nd, 2026 12:15 | by

MBON screen of MB399 for MBON02, MB050B for MBON15, MB027B for MBON17 all crossed with TnTe and WTB x TnTe for the negative control. Flies are tethered in torque meter and one turning domain is punished with a hot laser invisible to the fly. Each turning domain is also associated with an arena color. The lights are turned off at the last two periods. Wild type flies fail to learn to avoid the punished domain after the training periods in these conditions. A positive PI-score in the 12th period indicates that the fly learned.
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Registration of confocal images to brain template

on Monday, June 15th, 2026 12:54 | by

Brain1 :

Brain 3:

Brain 8:

To Do:

  • Verify transformation via QC (Overlay etc.)
  • Capture brains at higher resolution
  • Apply transformation to GFP channel > identify PPL1 neurons?

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Category: Anatomy | No Comments

TH Driver lines larval expression verification

on Friday, June 12th, 2026 2:11 | by

MB032B>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

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TH Driver lines adult expression verification

on Friday, June 12th, 2026 1:58 | by

MB299B>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

Janelia Fly Light Split-GAL4 reference

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TH Driver lines larval expression verification

on Tuesday, June 9th, 2026 12:40 | by

MB304B>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

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MBON Screen progress 01.06.2026

on Monday, June 1st, 2026 12:59 | by

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MBON screen progress 26.05.2026

on Tuesday, May 26th, 2026 9:05 | by

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TH Driver lines larval expression verification

on Friday, May 22nd, 2026 12:57 | by

MB299B>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

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MBON screen progress 18.05.2026

on Monday, May 18th, 2026 12:32 | by

PI- values for world learning torque experiments. As usual the flies were tethered and subjected to adverse stimuli by laser dependent on torque domain, which also controlled arena color. Flies that show positive PI at the end indicate premature habit formation as WT flies don’t learn under these conditions in this time frame.
Same as above except the pretest preference has been subtraced
Current p-values for all the tested groups. Should be 25-30 flies at trial end.
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Trans-tango repeat from previous week

on Monday, May 18th, 2026 12:21 | by

I was not able get any trans-tango signal from my reciprocal MB399B crosses so I repeated the experiment with anti-RFP antibody instead of anti-HA antibody

77124xMB399B (MBON02 reciprocal cross) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-RFP (2nd: anti-rat 555), nc82 (2nd: anti-mouse 650)

MBON02 trans-tango images from Kaun Lab as reference:

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