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MBON screen progress 21.09.2026

on Monday, September 21st, 2026 12:50 | by Fridrik Kjartansson

MBON screen progress update 21.09.2026: Due to RIGeL summer school last week, I was only able to run two experiments, obtaining five flies, two of which had to be cut due to quality control.

Category: crosses, flight, Habit formation, MBON, MBON, MBON, Operant learning, operant self-learning, World learning | No Comments

MBON screen progress 14.09.2026

on Monday, September 14th, 2026 1:07 | by Fridrik Kjartansson

Category: MBON, MBON, Operant learning, World learning | No Comments

Verification of RFP expression in stocks 539 and 550

on Friday, September 4th, 2026 3:56 | by Fridrik Kjartansson

After some failed attempts at a visualizing co-localization of FoxP and aPKC using a cross of stock 504 and 539/550, I grew suspicious of the RFP co-expression of FoxP. I visualized the CNS’ of both 539 and 550 in the confocal microscope. The image on the left is stock 550 and the image on the right 539. Stock 539 doesn’t seem to express any RFP but 550 looks fine so I’ll be repeating the cross using 550 when I get a chance. Rat anti-RFP primary antibody and 555 anti-rat secondary antibody were used as opposed to mouse anti-mcherry from previous experiment.

Category: Anatomy, Foxp | No Comments

MBON screen progress

on Monday, July 27th, 2026 12:43 | by Fridrik Kjartansson

Category: flight, Habit formation, MBON, MBON, MBON, Operant learning, Operant reinforcment, operant self-learning, World learning | No Comments

MB399Bxtrans-tango (BDSC: 77124)

on Monday, June 29th, 2026 10:23 | by Ipek Subay

antibodies: rabbit anti-GFP, rat anti-RFP, mouse anti-brp, anti-rabbit 488, anti- rat 650, anti mouse, 555

composite image:

trans-tango channel:

Category: Anatomy, MBON, trans-tango | No Comments

MBON screen progress 29.06.2026

on Monday, June 29th, 2026 9:50 | by Fridrik Kjartansson

Category: flight, Habit formation, MBON, MBON, MBON, Operant learning, operant self-learning, World learning | No Comments

TH Driver lines adult expression verification

on Friday, June 26th, 2026 11:23 | by Radostina Lyutova

MB304B>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

Janelia Fly Light Split-GAL4 reference

Category: Anatomy, Biogenic Amines, DAN, TH | No Comments

MBON screen progress 19.06.2026

on Monday, June 22nd, 2026 12:15 | by Fridrik Kjartansson

MBON screen of MB399 for MBON02, MB050B for MBON15, MB027B for MBON17 all crossed with TnTe and WTB x TnTe for the negative control. Flies are tethered in torque meter and one turning domain is punished with a hot laser invisible to the fly. Each turning domain is also associated with an arena color. The lights are turned off at the last two periods. Wild type flies fail to learn to avoid the punished domain after the training periods in these conditions. A positive PI-score in the 12th period indicates that the fly learned.

Category: flight, Habit formation, MBON, MBON, Memory, Mushroom Body, Operant learning, Operant reinforcment, operant self-learning, World learning | No Comments

Registration of confocal images to brain template

on Monday, June 15th, 2026 12:54 | by Daniel Döringer

These are my first attempts to register expression patterns of the TH-C-AD;TH-D-DBD split-Gal4 driver to a standard brain template (Image 1, green) via the ‘ants’ package. The idea is to register the nc82 channel to the template to generate a transformation matrix (transformed nc82 signal is shown in red) than can be applied to the GFP channel. The ultimate goal is to use these transformed GFP stainings to identify neurons in the fly brain.