First feeding essay and wiggle factor
on Monday, March 2nd, 2026 12:51 | by Johanna Jäger
On the left, there are the results of the experimental group: social isolation, with the usual R Skript. The right side shows the same results but with an adjusted “flat threshold” (for movement) of 0.4 instead of 0.9.
First Feeding essay for experimental group “crowding” and “sleep deprivation”:
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stressor: heat
on Monday, March 2nd, 2026 12:33 | by Johanna Jäger
stressor: heat (37°C; 6min)
POS_CTRL: control group
Gr28bd+TrpA1(heat): experimental group
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TH Driver lines larval expression verification
on Monday, March 2nd, 2026 12:28 | by Radostina Lyutova
NP0047-Gal4/CyO>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
TH-D’-Gal4>UAS>>mCD8::GFP,UAS>>mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
TH-D1-Gal4>UAS>>mCD8::GFP,UAS>>mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
Category: Anatomy, Biogenic Amines, Larvae | No Comments
Salt (1.5 M) avoidance tests in larvae (DANc1) under blue and red light (N = 12)
on Sunday, March 1st, 2026 4:56 | by Christoph Kumpfmüller
Category: crosses, genetics, Larvae, Mushroom Body, Optogenetics | No Comments
WTB Test for Torquemeter February 19-20
on Friday, February 27th, 2026 5:26 | by Ipek Subay
10 flies completed out of 36 flies.
Optomotor traces (right/left) at start of experiment
Optomotor traces (right/left) at end of experiment
Performance Index bar plot with SEM:
Performance Index bar plot – preference subtracted:
Performance Index box&dotplot without notches:
Statistical tests of single groups against zero
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Retro-Tango endogenous GFP Expression
on Friday, February 27th, 2026 5:20 | by Ipek Subay
On the right: Endogenous GFP expression (without antibody) from retro tango flies (BDSC: 99661) crossed with GF-Gal4 (BDSC: 79602) driver line.
On the left: GFP expression in retro-tango x GF-Gal4 flies from retro-tango publication ( https://doi.org/10.7554/eLife.85041 )
Same image but in retro-tango signal channel:
Category: Anatomy, retro-tango | No Comments
RU_486 induced mCD8-GFP
on Monday, February 23rd, 2026 12:56 | by Julia Schulz
Category: Uncategorized | No Comments
FoxP-RFP stock test
on Monday, February 23rd, 2026 12:53 | by Fridrik Kjartansson
Category: Foxp | No Comments
Torque measurements 21.02 & 22.02
on Monday, February 23rd, 2026 12:21 | by Fridrik Kjartansson
Category: flight, Habit formation, operant self-learning, Optomotor response | No Comments
Stressors: cold and crowding
on Sunday, February 22nd, 2026 11:48 | by Johanna Jäger
For the stressor ‘cold’ the flies were kept on 18°C for 48h on ATR.
stressor: cold (18°C for 48h)
POS_CTRL: control group
Gr28bd+TrpA1(cold): experimental group
For the stressor ‘crowding’ ca. 60-65 flies were kept in one vial for 48h on ATR.
The effect of crowding was created by the volume of air available to the flies in the vial. This was altered via change in the height of the cotton plug in the vial. The control group had the cotton plug at the usual height of ca. 60mm above the food in the vial and the experimental group only 20mm.
stressor: crowding (maintained in smaller volume for 48h on ATR)
POS_CTRL: control group
Gr28bd+TrpA1(Crow): experimental group
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