MBON screen progress 18.05.2026

on Monday, May 18th, 2026 12:32 | by

PI- values for world learning torque experiments. As usual the flies were tethered and subjected to adverse stimuli by laser dependent on torque domain, which also controlled arena color. Flies that show positive PI at the end indicate premature habit formation as WT flies don’t learn under these conditions in this time frame.
Same as above except the pretest preference has been subtraced
Current p-values for all the tested groups. Should be 25-30 flies at trial end.
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MBON screen progress 11.05.2026

on Monday, May 11th, 2026 12:25 | by

PI-values unsubtracted:

Results from premature habit formation torque measurements of four drosophila crosses: MB399B, MB050B & MB027B gal4 driver lines crossed with UAS-Tetanus toxin lines and Wild type Berlin crossed with UAS-TnT as control. Which line is which is still unknown as the screen is incomplete and researcher must remain blind. It appears two crosses strongly form habits prematurely, one doesn’t and one is still on the borderline.

PI-values subtracted:

Same results as above except with pretest bias subtracted.

p-values:

p-values for the different groups
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Long form habit formation pooled data 16-20th & 23-27th of March 2026

on Monday, March 30th, 2026 12:07 | by

Performance index values for long sequence habit formation trial
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Habit formation long, 15.3-20.3.2026

on Monday, March 23rd, 2026 10:33 | by

Results from week 15-20.3.2026 of the long form habit formation trial. Only 6 flies out of 24 managed to successfully complete the trial. Avoidance was improved by increasing laser voltage.
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Color learning 13.03.2026

on Monday, March 16th, 2026 10:38 | by

Results from first attempt at the habit formation trial. Strong positive preference in the beginning as well as low number of successful flies obscures the results. Reason for the disturbed OR at the end is that the offset for one fly was accidentally shifted while attempting to restart flight with a brush.
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Torque Measurements 24-27.2.2026

on Monday, March 2nd, 2026 4:39 | by

Results from tethered flight in torque meter. Fly fixed to hook and flight torque measured while laser punishes either left or right turning. Higher PI score indicates longer stay in unpunished domain, orange colored periods are punished. Higher scores in period 9, 12 and 13 indicates learning. Fly genotype is Wild type Berlin, N=16.
Same as above, with PI adjusted to factor in initial domain preference.
A low P-score indicates that the result is statistically significant and the flies indeed learned.
Pie charts indicate efficiency of experiment success. Blue wedge represents flies that completed trial, other colors represent flies that failed. Efficiency still low, using laser at 100% duty cycle often causes flies to stop flying unless directed at exactly the right position on their antennae.

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2.Yaw torque

on Monday, March 2nd, 2026 1:08 | by

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1. aPKC_TurboID_line

on Monday, March 2nd, 2026 1:07 | by

Generation of TurboID-linker-aPKC construct

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Torque measurements 21.02 & 22.02

on Monday, February 23rd, 2026 12:21 | by

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Torque measurements 12.02.2026

on Friday, February 13th, 2026 12:38 | by

Torque results 12.02.2026, learning is abolished and many of the flies died. Think improper feeding is to blame. Granulated sugar had become encrusted and possibly inaccessible to the flies, potentially resulting in starvation. In the future, I’ll resolve to use less sugar and to clean more frequently to prevent this kind of buildup.
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