Habit formation | .:lab.brembs.net:.

MBON Screen progress 01.06.2026

on Monday, June 1st, 2026 12:59 | by Fridrik Kjartansson

Category: flight, Habit formation, MBON, MBON, MBON, Operant learning, Operant reinforcment, operant self-learning, World learning | No Comments

MBON screen progress 26.05.2026

on Tuesday, May 26th, 2026 9:05 | by Fridrik Kjartansson

Category: flight, Habit formation, MBON, MBON, Operant learning, Operant reinforcment, operant self-learning, World learning | No Comments

MBON screen progress 18.05.2026

on Monday, May 18th, 2026 12:32 | by Fridrik Kjartansson

PI- values for world learning torque experiments. As usual the flies were tethered and subjected to adverse stimuli by laser dependent on torque domain, which also controlled arena color. Flies that show positive PI at the end indicate premature habit formation as WT flies don’t learn under these conditions in this time frame.

Same as above except the pretest preference has been subtraced

Current p-values for all the tested groups. Should be 25-30 flies at trial end.

Category: crosses, Habit formation, MBON, MBON, MBON, Memory, Operant learning, Operant reinforcment, operant self-learning, World learning | No Comments

Trans-tango repeat from previous week

on Monday, May 18th, 2026 12:21 | by Ipek Subay

I was not able get any trans-tango signal from my reciprocal MB399B crosses so I repeated the experiment with anti-RFP antibody instead of anti-HA antibody

77124xMB399B (MBON02 reciprocal cross) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-RFP (2nd: anti-rat 555), nc82 (2nd: anti-mouse 650)

MBON02 trans-tango images from Kaun Lab as reference:

Category: Anatomy, Habit formation, MBON, MBON | No Comments

Tango imaging

on Monday, May 11th, 2026 12:32 | by Ipek Subay

99661xSS98650 (retro tango imaging for b3 mn) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-RFP (2n: anti-rat 555), nc82 (2nd: anti-mouse 650)

GFP channel:

somehow my antibodies decided to work on retro tango flies even though I did not change anything in the protocol

retro-tango channel:

b3 presynaptic neurons from BANC dataset:

99661xSS48311 (b3 mn) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-RFP (2n: anti-rat 555), nc82 (2nd: anti-mouse 650)

GFP:

Retro-tango:

99661xSS40980 (b1 mn) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-RFP (2n: anti-rat 555), nc82 (2nd: anti-mouse 650)

GFP:

retro-tango:

b1 presynaptic neurons from BANC dataset:

77124xMB399B (MBON02 reciprocal cross) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-HA (2n: anti-rat 555), nc82 (2nd: anti-mouse 650)

GFP:

trans-tango:

trans-tango maximum intensity projection:

Category: Habit formation, MBON, Motor neurons | No Comments

MBON screen progress 11.05.2026

on Monday, May 11th, 2026 12:25 | by Fridrik Kjartansson

PI-values unsubtracted:

Results from premature habit formation torque measurements of four *drosophila* crosses: MB399B, MB050B & MB027B gal4 driver lines crossed with UAS-Tetanus toxin lines and Wild type Berlin crossed with UAS-TnT as control. Which line is which is still unknown as the screen is incomplete and researcher must remain blind. It appears two crosses strongly form habits prematurely, one doesn’t and one is still on the borderline.

PI-values subtracted:

Same results as above except with pretest bias subtracted.

p-values:

Category: crosses, flight, Habit formation, MBON, MBON, MBON, Operant learning, operant self-learning, World learning | No Comments

Long form habit formation pooled data 16-20th & 23-27th of March 2026

on Monday, March 30th, 2026 12:07 | by Fridrik Kjartansson

Performance index values for long sequence habit formation trial

Category: flight, Habit formation, Operant learning, operant self-learning, World learning | No Comments

Short habit formation trial 11.03.2026

on Monday, March 16th, 2026 10:47 | by Fridrik Kjartansson

Short form habit formation trial, indicating operant learning is abolished/delayed in the presence of external associative stimuli.

Category: flight, Habit formation, Operant learning | No Comments

Color learning 13.03.2026

on Monday, March 16th, 2026 10:38 | by Fridrik Kjartansson

Results from first attempt at the habit formation trial. Strong positive preference in the beginning as well as low number of successful flies obscures the results. Reason for the disturbed OR at the end is that the offset for one fly was accidentally shifted while attempting to restart flight with a brush.

Category: flight, Habit formation, Operant learning, operant self-learning | No Comments

Habit Formation Wildtype Test 02.03-06.03

on Tuesday, March 10th, 2026 10:45 | by Ipek Subay

I wanted to try Habit formation with wildtype and tested 4 wheels of flies (48) over the last week. Unfortunately, I did a mistake when setting the timeslots file for right torque so it had an additional testing period at P13. So analyzed the left and right individually:

For Punishment on Left Torque:

Optomotor traces (right/left) at start of experiment: