on Monday, January 29th, 2024 11:30 | by Devashree Joglekar
Over the course, control and test lines were experimented with under ‘Joystick’. The test lines include 13:0273-Gal 4 line, SS56699 line, and TH-C-AD; TH-D-DBD line. The tests were done under red and yellow light conditions.
Red light conditions:
Yellow light conditions:
on Monday, September 25th, 2023 11:07 | by Maja Achatz
The first experiment tested the activation of KCs with simultaneous ablation of pPAM which showed the following results:
Green: UAS-ChR2-XXL; LexAop-rpr x 58E02-LexA; H24-Gal4
Lightgrey: UAS-ChR2-XXL; LexAop-rpr x H24-Gal4
Darkgrey: UAS-ChR2-XXL x 58E02-LexA; H24-Gal4
The next experiment was conducted with the split-Gal4 line MB054B (DAN-f1/DAN-g1) and showed the following:
on Monday, September 11th, 2023 9:52 | by Luisa Guyton
on Saturday, September 2nd, 2023 11:09 | by Maja Achatz
With the focus on dopaminergic neurons, I conducted two experiments with two different driver lines to see how the naive gustatory behavior in Drosophila larvae is affected.
The first cross I tested was R58E02-Gal4 x UAS-ChR2-XXL, which gave me the following results:
As seen in the figures, there was no effect under blue light.
For the second experiment I crossed TH-Gal4 x UAS-ChR2-XXL with these results:
With this experiment, there was an effect on gustatory behavior as there is a clear significant difference between the experimental group and the control group.
on Monday, August 7th, 2023 12:46 | by Maja Achatz
The following plots show the results after I increased the salt concentration from 1,5M to 2,5M. It shows that the difference between the control groups and experimental group are not significant anymore.
on Monday, July 24th, 2023 9:24 | by Maja Achatz
on Friday, July 7th, 2023 12:57 | by Maja Achatz
on Monday, June 26th, 2023 1:45 | by Maja Achatz
The first plot of each group show my results from the week before, and the ones beneath show the final results for red and blue light, after filling the sample size up to 20n.
for comparison, this plot shows the control groups for the remaining 8n I had left separately:
on Tuesday, May 30th, 2023 1:31 | by Luisa Guyton
The following figures show the results of the joystick test using the same test line as before. In addition, the flies were fed not only ATR but also 3IY (3-iodo-L-tyrosine), an inhibitor of dopamine synthesis. This was done to determine whether the previously observed effect was due to dopamine alone and could therefore be suppressed by the inhibitor, or whether other factors also influenced the flies’ behaviour in the joystick test.