Results for Dop1R1-RNAi, Amon-RNAi and the course data
on Tuesday, May 21st, 2024 8:11 | by Maja Achatz
Firstly, I did an experiment where Dop1R1 was knocked down via RNAi. The following shows the results:
![](https://lab.brembs.net/wp/wp-content/uploads/2024/05/Bildschirmfoto-2024-05-21-um-08.00.55-1024x793.png)
For the next experiment, the controls groups were the same, however in the experimental group Amon was down regulated. These are the results:
![](https://lab.brembs.net/wp/wp-content/uploads/2024/05/Bildschirmfoto-2024-05-21-um-08.02.01-1-1024x782.png)
Lastly, in the course the data for the larvae experiment was collected only under blue light, so I used the same crossings and tested them under red light to use as a control.
![](https://lab.brembs.net/wp/wp-content/uploads/2024/05/Bildschirmfoto-2024-05-21-um-08.03.12-1024x794.png)
Category: Kenyon cells, Larve, Mushroom Body, Optogenetics, Uncategorized | No Comments
Results for MB145B, SS01716 and SS02180
on Monday, April 8th, 2024 10:33 | by Maja Achatz
All figures show the results for preference tests (salt/pure) conducted under red and blue light for different gal4-lines.
MB145B:
![](https://lab.brembs.net/wp/wp-content/uploads/2024/04/Bildschirmfoto-2024-04-08-um-10.27.01-1024x798.png)
SS01716:
![](https://lab.brembs.net/wp/wp-content/uploads/2024/04/Bildschirmfoto-2024-04-08-um-10.27.39-1024x799.png)
SS02180:
![](https://lab.brembs.net/wp/wp-content/uploads/2024/04/Bildschirmfoto-2024-04-08-um-10.28.32-1024x778.png)
Category: DAN, Larve, Mushroom Body, Optogenetics | No Comments
Results for amon-RNAi and MB143B
on Monday, February 26th, 2024 12:17 | by Maja Achatz
The first figure shows the results for the preference test (salt/pure) conducted with amon-RNAi only under red light.
![](https://lab.brembs.net/wp/wp-content/uploads/2024/02/amonRNAi_ready.png)
For the next experiment I conducted the same preference test but with the split-gal4 line MB143B under red and blue light.
![](https://lab.brembs.net/wp/wp-content/uploads/2024/02/MB143B_ready-1024x777.png)
Category: Larve, Mushroom Body, Optogenetics | No Comments
Final ‘Joystick’ test results
on Monday, January 29th, 2024 11:30 | by Devashree Joglekar
Over the course, control and test lines were experimented with under ‘Joystick’. The test lines include 13:0273-Gal 4 line, SS56699 line, and TH-C-AD; TH-D-DBD line. The tests were done under red and yellow light conditions.
Red light conditions:
![](https://lab.brembs.net/wp/wp-content/uploads/2024/01/Screenshot-845-1024x392.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2024/01/Control-No-ATR-results-1024x406.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2024/01/130273gal4-line-result-1024x399.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2024/01/SS56699-line-results-1024x396.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2024/01/THCAD-THDDBD-line-results-1024x398.png)
Yellow light conditions:
![](https://lab.brembs.net/wp/wp-content/uploads/2024/01/Control-line-results-1024x379.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2024/01/Control-no-ATR-results-1-1024x391.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2024/01/130273-gal-4-line-results-1024x390.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2024/01/SS56699-line-results-1-1024x393.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2024/01/THCAD-THDDBD-line-results-1-1024x390.png)
Category: Operant reinforcment, Optogenetics | No Comments
Results for MB+DANs and MB054B
on Monday, September 25th, 2023 11:07 | by Maja Achatz
The first experiment tested the activation of KCs with simultaneous ablation of pPAM which showed the following results:
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/MBDAN-fur-lab.brembs-1-1024x784.png)
Green: UAS-ChR2-XXL; LexAop-rpr x 58E02-LexA; H24-Gal4
Lightgrey: UAS-ChR2-XXL; LexAop-rpr x H24-Gal4
Darkgrey: UAS-ChR2-XXL x 58E02-LexA; H24-Gal4
The next experiment was conducted with the split-Gal4 line MB054B (DAN-f1/DAN-g1) and showed the following:
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/MB054B_labbrembs-1024x1024.jpg)
Category: Kenyon cells, Larve, Mushroom Body, Optogenetics | No Comments
Verification TH-C-AD;TH-D-DBD
on Monday, September 11th, 2023 9:52 | by Luisa Guyton
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/Abbildung-8.jpg)
Category: Anatomy, DAN, genetics, Kenyon cells, Optogenetics | No Comments
Optogenetic stimulation of DANs
on Saturday, September 2nd, 2023 11:09 | by Maja Achatz
With the focus on dopaminergic neurons, I conducted two experiments with two different driver lines to see how the naive gustatory behavior in Drosophila larvae is affected.
The first cross I tested was R58E02-Gal4 x UAS-ChR2-XXL, which gave me the following results:
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/R58E02_redlight-1024x1024.jpg)
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/R58E02_Gal4_bluelight-1024x1024.jpg)
As seen in the figures, there was no effect under blue light.
For the second experiment I crossed TH-Gal4 x UAS-ChR2-XXL with these results:
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/Th_Gal4_redlight-1024x1024.jpg)
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/TH_Gal4_bluelight-1024x1024.jpg)
With this experiment, there was an effect on gustatory behavior as there is a clear significant difference between the experimental group and the control group.
Category: DAN, Larve, Optogenetics, PAM | No Comments
Preferences with higher salt concentration
on Monday, August 7th, 2023 12:46 | by Maja Achatz
The following plots show the results after I increased the salt concentration from 1,5M to 2,5M. It shows that the difference between the control groups and experimental group are not significant anymore.
![](https://lab.brembs.net/wp/wp-content/uploads/2023/08/Salz_25M_redlight-1024x1024.jpg)
![](https://lab.brembs.net/wp/wp-content/uploads/2023/08/Salz_25M_bluelight-1024x844.jpg)
Category: Larve, Mushroom Body, Optogenetics | No Comments
Pure vs Sugar Preference results
on Monday, July 24th, 2023 9:24 | by Maja Achatz
![](https://lab.brembs.net/wp/wp-content/uploads/2023/07/PvsZ_redlight_final-1024x1024.jpg)
![](https://lab.brembs.net/wp/wp-content/uploads/2023/07/PvsZ_bluelight_final-1024x1024.jpg)
Category: Larve, Mushroom Body, Optogenetics | No Comments
Pure vs salt preference results
on Friday, July 7th, 2023 12:57 | by Maja Achatz
![](https://lab.brembs.net/wp/wp-content/uploads/2023/07/PvsS_redlight_final1024_1-2.jpg)
![](https://lab.brembs.net/wp/wp-content/uploads/2023/07/PvsS_bluelight_final1024_1.jpg)
Category: Larve, Optogenetics | No Comments