Final ‘Joystick’ test results

on Monday, January 29th, 2024 11:30 | by

Over the course, control and test lines were experimented with under ‘Joystick’. The test lines include 13:0273-Gal 4 line, SS56699 line, and TH-C-AD; TH-D-DBD line. The tests were done under red and yellow light conditions.

Red light conditions:

Yellow light conditions:

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Results for MB+DANs and MB054B

on Monday, September 25th, 2023 11:07 | by

The first experiment tested the activation of KCs with simultaneous ablation of pPAM which showed the following results:

Green: UAS-ChR2-XXL; LexAop-rpr x 58E02-LexA; H24-Gal4

Lightgrey: UAS-ChR2-XXL; LexAop-rpr x H24-Gal4

Darkgrey: UAS-ChR2-XXL x 58E02-LexA; H24-Gal4

The next experiment was conducted with the split-Gal4 line MB054B (DAN-f1/DAN-g1) and showed the following:

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Verification TH-C-AD;TH-D-DBD

on Monday, September 11th, 2023 9:52 | by

Confocal images with anti-GFP and anti-Brp staining of TH-C-AD;TH-D-DBD > mCD::GFP fly brains: A: 4 PPM 2 DANs per hemisphere marked with blue circles, three clusters of Kenyon cell bodies per hemisphere marked with white arrows. B: Five visible cell bodies of unidentified neurons marked with a blue circle in the left hemisphere. The neurons project into the lobula plate and the medulla. Strong fluorescence of cell bodies of the Kenyon cells projecting into the Mushroom bodies is visible. C: Unidentified neurons projecting into structures outside of the optic lobes especially the ventral lateral protocerebrum.
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Optogenetic stimulation of DANs

on Saturday, September 2nd, 2023 11:09 | by

With the focus on dopaminergic neurons, I conducted two experiments with two different driver lines to see how the naive gustatory behavior in Drosophila larvae is affected.

The first cross I tested was R58E02-Gal4 x UAS-ChR2-XXL, which gave me the following results:

As seen in the figures, there was no effect under blue light.

For the second experiment I crossed TH-Gal4 x UAS-ChR2-XXL with these results:

With this experiment, there was an effect on gustatory behavior as there is a clear significant difference between the experimental group and the control group.

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Preferences with higher salt concentration

on Monday, August 7th, 2023 12:46 | by

The following plots show the results after I increased the salt concentration from 1,5M to 2,5M. It shows that the difference between the control groups and experimental group are not significant anymore.

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Pure vs Sugar Preference results

on Monday, July 24th, 2023 9:24 | by

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Pure vs salt preference results

on Friday, July 7th, 2023 12:57 | by

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Preference results: pure vs salt

on Monday, June 26th, 2023 1:45 | by

The first plot of each group show my results from the week before, and the ones beneath show the final results for red and blue light, after filling the sample size up to 20n.

Red light:

for comparison, this plot shows the control groups for the remaining 8n I had left separately:

Blue light:

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Joystick Results for 2-minute-testing

on Tuesday, May 30th, 2023 1:31 | by

The following figures show the results of the joystick test using the same test line as before. In addition, the flies were fed not only ATR but also 3IY (3-iodo-L-tyrosine), an inhibitor of dopamine synthesis. This was done to determine whether the previously observed effect was due to dopamine alone and could therefore be suppressed by the inhibitor, or whether other factors also influenced the flies’ behaviour in the joystick test.

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Red light Joystick Results

on Monday, April 3rd, 2023 12:33 | by

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