Optogenetic stimulation of DANs

on Saturday, September 2nd, 2023 11:09 | by

With the focus on dopaminergic neurons, I conducted two experiments with two different driver lines to see how the naive gustatory behavior in Drosophila larvae is affected.

The first cross I tested was R58E02-Gal4 x UAS-ChR2-XXL, which gave me the following results:

As seen in the figures, there was no effect under blue light.

For the second experiment I crossed TH-Gal4 x UAS-ChR2-XXL with these results:

With this experiment, there was an effect on gustatory behavior as there is a clear significant difference between the experimental group and the control group.

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Preferences with higher salt concentration

on Monday, August 7th, 2023 12:46 | by

The following plots show the results after I increased the salt concentration from 1,5M to 2,5M. It shows that the difference between the control groups and experimental group are not significant anymore.

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Pure vs Sugar Preference results

on Monday, July 24th, 2023 9:24 | by

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Pure vs salt preference results

on Friday, July 7th, 2023 12:57 | by

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Preference results: pure vs salt

on Monday, June 26th, 2023 1:45 | by

The first plot of each group show my results from the week before, and the ones beneath show the final results for red and blue light, after filling the sample size up to 20n.

Red light:

for comparison, this plot shows the control groups for the remaining 8n I had left separately:

Blue light:

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Preference Results for Canton S

on Monday, June 19th, 2023 1:20 | by

The following shows the preference and avoidance towards Sugar and Salt for the Canton S larvae. In addition, the larvae were tested unter blue/room light, to see if there were any differences.

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Preference Results for Canton S larvae, Pure vs Sugar and Pure vs Salt

on Monday, June 12th, 2023 1:49 | by

The following figures show the results of the preference tests with Canton S. I tested Pure vs Sugar and Pure vs Salt under two different circumstances. I tested the larvae in normal light and also under blue light.

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Latest food patch experiments on Rover/Sitter larvae

on Sunday, December 20th, 2020 5:31 | by

Food Patch Experiment

For Control larvae from the 11.12.2020 and 14.12.2020. There is nearly no difference between both groups.

Time of first movement function

Additional function to plot the time when the larva starts moving. The Data from last week was used
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ELAV-Gal4;Tub-Gal80: Temperature switch at embryo stage

on Tuesday, December 15th, 2020 2:34 | by

We phenocopied the results of the ELAV-Gal4 cross.

Now we are waiting for the results of the Temperature switch at the second instar larvae stage

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Category: Foxp, Larve | No Comments

Further testing of the For Control Group

on Monday, December 14th, 2020 11:10 | by

Food patch experiment

Barplot of the For Control Group with additional n=4 .

Distance tracking

The larvae were filmed for one minute. Larvae that reached the edge of the petri dish before one minute were discarded.
Video recording was started as soon as the larvae where placed on the agar plate.
Same data cut after 30 s.
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