Optogenetic stimulation of DANs
on Saturday, September 2nd, 2023 11:09 | by Maja Achatz
With the focus on dopaminergic neurons, I conducted two experiments with two different driver lines to see how the naive gustatory behavior in Drosophila larvae is affected.
The first cross I tested was R58E02-Gal4 x UAS-ChR2-XXL, which gave me the following results:
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/R58E02_redlight-1024x1024.jpg)
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/R58E02_Gal4_bluelight-1024x1024.jpg)
As seen in the figures, there was no effect under blue light.
For the second experiment I crossed TH-Gal4 x UAS-ChR2-XXL with these results:
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/Th_Gal4_redlight-1024x1024.jpg)
![](https://lab.brembs.net/wp/wp-content/uploads/2023/09/TH_Gal4_bluelight-1024x1024.jpg)
With this experiment, there was an effect on gustatory behavior as there is a clear significant difference between the experimental group and the control group.
Category: DAN, Larve, Optogenetics, PAM | No Comments
Lines Verification
on Monday, September 12th, 2022 9:55 | by Silvia Marcato
Category: Anatomy, DAN, Kenyon cells, Mushroom Body, PAM | No Comments