Results for Dop1R1-RNAi, Amon-RNAi and the course data

on Tuesday, May 21st, 2024 8:11 | by

Firstly, I did an experiment where Dop1R1 was knocked down via RNAi. The following shows the results:

For the next experiment, the controls groups were the same, however in the experimental group Amon was down regulated. These are the results:

Lastly, in the course the data for the larvae experiment was collected only under blue light, so I used the same crossings and tested them under red light to use as a control.

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First data from aPKC knock-out in FoxP neurons

on Monday, May 13th, 2024 8:49 | by

When Andi tested his aPKC CRISPR knock-outs, he used the Tang torque meter setup, where the OMRs aren’t recorded. So I’m attempting to replicate his results and compare OMRs between groups.

It looks like I need to tweak the graph in some way to make the density plots show up. Obviously, N is too low to say anything yet, but I need to work on the plot.

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Bachelor Blog / #5 no learning :(

on Monday, September 11th, 2023 12:21 | by

Below you find the data from my experimental rounds A and B:

-> learning scores of the parental flies from experimental round A and B

-> learning scores of the trained parent´s offspring only from experimental round A

-> learning scores of the untrained parent´s offspring only from experimental round A

The results confuse me a lot and I am happy to discuss reasons :) However the offspring of the round-B will be ready for testing by the end of this week so there is still some data to collect…

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Success: rsh Stock has rsh1 Mutation

on Monday, August 7th, 2023 11:11 | by

Via gDNA analysis and PCR was the specific area of the rsh gene extracted and amplified where the nucleotide substitution: C to T (Folkers et al., 2006) should be for the rsh1 mutation. The amplicon was Sanger sequenced which proved the nucleotide substitution.

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Creating gRNAs via PCR

on Friday, July 7th, 2023 6:38 | by

1% Agarose gel with 100bp marker and PCR1 rsh, PCR2 rsh and PCR3 rsh or PCR1 rut, PCR2 rut and PCR3 rut, respectively.
The template pCFD6 was used with a concentration of 640 pg/µl.
1% Agarose gel with 100bp marker and PCR1 rsh, PCR2 rsh and PCR3 rsh or PCR1 rut, PCR2 rut and PCR3 rut, respectively.
The template pCFD6 was used with a concentration of 64 pg/µl (1:10 dilution).
1% Agarose gel with 100bp marker and PCR1 rsh, PCR2 rsh and PCR3 rsh or PCR1 rut, PCR2 rut and PCR3 rut, respectively.
The template pCFD6 was used with a concentration of 64 pg/µl (1:10 dilution).
50µl of 5xQ5 High GC Enhancer was added to the PCR mix.
1% Agarose gel with 100bp marker and PCR1 rsh, PCR1 rut.
The template pCFD6 was used with a concentration of 128 pg/µl (1:5 dilution).
50µl of 5xQ5 High GC Enhancer was added to the PCR mix.
1% Agarose gel with 100bp marker and PCR1 rsh, PCR2 rsh and PCR3 rsh or PCR1 rut, PCR2 rut and PCR3 rut, respectively.
The template pCFD6 was used with a concentration of 640 pg/µl.
The Phusion DNA Polymerase was used instead of the Q5 High-Fidelity DNA Polymerase.
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Flight Duration

on Monday, January 30th, 2023 12:40 | by

21/12/2022
TntG expression
30/01/2023
TntE expression
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Kibra knockout, adult

on Monday, December 19th, 2022 11:15 | by

Knockout of Kibra in adult flies.

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Kibra developmental knockout

on Monday, September 5th, 2022 9:39 | by

Knockout of Kibra during development.

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Enes and Aslıhan joystick results of ATR and NOATR Control, ATR SifaG4 and ATR 13.0273 for yellow light.

on Wednesday, August 24th, 2022 10:32 | by

New experiments added for yellow light, ATR and NOATR Control group, ATR Sifa-G4 and ATR 13.0273-Gal4.

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Enes and Aslıhan Joystick Experiment of Control groups , Sifa-G4, 13.0273-Gal4

on Monday, August 22nd, 2022 11:48 | by

These are the result of Joystick experiements of Control group with ATR and without ATR yellow and red light, SifaG4 group with ATR, yellow and red light, 13.0273Gal4 group with ATR, yellow and red light.

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