Results for red light

Results for yellow light

With the modified laser settings I was able to test 3 more wtb. Here are the results:

–> yaw torque

Firstly, I did an experiment where Dop1R1 was knocked down via RNAi. The following shows the results:

For the next experiment, the controls groups were the same, however in the experimental group Amon was down regulated. These are the results:

Lastly, in the course the data for the larvae experiment was collected only under blue light, so I used the same crossings and tested them under red light to use as a control.

When Andi tested his aPKC CRISPR knock-outs, he used the Tang torque meter setup, where the OMRs aren’t recorded. So I’m attempting to replicate his results and compare OMRs between groups.

It looks like I need to tweak the graph in some way to make the density plots show up. Obviously, N is too low to say anything yet, but I need to work on the plot.

Below you find the data from my experimental rounds A and B:

-> learning scores of the parental flies from experimental round A and B

-> learning scores of the trained parent´s offspring only from experimental round A

-> learning scores of the untrained parent´s offspring only from experimental round A

The results confuse me a lot and I am happy to discuss reasons :) However the offspring of the round-B will be ready for testing by the end of this week so there is still some data to collect…

Via gDNA analysis and PCR was the specific area of the rsh gene extracted and amplified where the nucleotide substitution: C to T (Folkers et al., 2006) should be for the rsh¹ mutation. The amplicon was Sanger sequenced which proved the nucleotide substitution.