Bachelor Blog / #5 no learning :(

on Monday, September 11th, 2023 12:21 | by

Below you find the data from my experimental rounds A and B:

-> learning scores of the parental flies from experimental round A and B

-> learning scores of the trained parent´s offspring only from experimental round A

-> learning scores of the untrained parent´s offspring only from experimental round A

The results confuse me a lot and I am happy to discuss reasons :) However the offspring of the round-B will be ready for testing by the end of this week so there is still some data to collect…

Print Friendly, PDF & Email

Success: rsh Stock has rsh1 Mutation

on Monday, August 7th, 2023 11:11 | by

Via gDNA analysis and PCR was the specific area of the rsh gene extracted and amplified where the nucleotide substitution: C to T (Folkers et al., 2006) should be for the rsh1 mutation. The amplicon was Sanger sequenced which proved the nucleotide substitution.

Print Friendly, PDF & Email

Creating gRNAs via PCR

on Friday, July 7th, 2023 6:38 | by

1% Agarose gel with 100bp marker and PCR1 rsh, PCR2 rsh and PCR3 rsh or PCR1 rut, PCR2 rut and PCR3 rut, respectively.
The template pCFD6 was used with a concentration of 640 pg/µl.
1% Agarose gel with 100bp marker and PCR1 rsh, PCR2 rsh and PCR3 rsh or PCR1 rut, PCR2 rut and PCR3 rut, respectively.
The template pCFD6 was used with a concentration of 64 pg/µl (1:10 dilution).
1% Agarose gel with 100bp marker and PCR1 rsh, PCR2 rsh and PCR3 rsh or PCR1 rut, PCR2 rut and PCR3 rut, respectively.
The template pCFD6 was used with a concentration of 64 pg/µl (1:10 dilution).
50µl of 5xQ5 High GC Enhancer was added to the PCR mix.
1% Agarose gel with 100bp marker and PCR1 rsh, PCR1 rut.
The template pCFD6 was used with a concentration of 128 pg/µl (1:5 dilution).
50µl of 5xQ5 High GC Enhancer was added to the PCR mix.
1% Agarose gel with 100bp marker and PCR1 rsh, PCR2 rsh and PCR3 rsh or PCR1 rut, PCR2 rut and PCR3 rut, respectively.
The template pCFD6 was used with a concentration of 640 pg/µl.
The Phusion DNA Polymerase was used instead of the Q5 High-Fidelity DNA Polymerase.
Print Friendly, PDF & Email

Flight Duration

on Monday, January 30th, 2023 12:40 | by

21/12/2022
TntG expression
30/01/2023
TntE expression
Print Friendly, PDF & Email

Kibra knockout, adult

on Monday, December 19th, 2022 11:15 | by

Knockout of Kibra in adult flies.

Print Friendly, PDF & Email

Kibra developmental knockout

on Monday, September 5th, 2022 9:39 | by

Knockout of Kibra during development.

Print Friendly, PDF & Email

Enes and Aslıhan joystick results of ATR and NOATR Control, ATR SifaG4 and ATR 13.0273 for yellow light.

on Wednesday, August 24th, 2022 10:32 | by

New experiments added for yellow light, ATR and NOATR Control group, ATR Sifa-G4 and ATR 13.0273-Gal4.

Print Friendly, PDF & Email

Enes and Aslıhan Joystick Experiment of Control groups , Sifa-G4, 13.0273-Gal4

on Monday, August 22nd, 2022 11:48 | by

These are the result of Joystick experiements of Control group with ATR and without ATR yellow and red light, SifaG4 group with ATR, yellow and red light, 13.0273Gal4 group with ATR, yellow and red light.

Print Friendly, PDF & Email

Enes & Aslı & Melis Control Groups

on Thursday, August 18th, 2022 10:38 | by

These are Joystick results of Control group flies with and without ATR, yellow and red light.
Print Friendly, PDF & Email

NorAUASChrimsonxGr28bdTrpA1_Red

on Monday, July 18th, 2022 9:15 | by

Print Friendly, PDF & Email

older posts