Added more flies to the aPKC knock-out in FoxP neurons. Now the knock-outs are close to zero, but one of the controls, too. Still too early to say much. The figure looks ok now, but the Bayes Factors get chopped off. need to fix this. As I’m, already working on some figures (histograms) in the code, I can fix this as well.

Firstly, I did an experiment where Dop1R1 was knocked down via RNAi. The following shows the results:

For the next experiment, the controls groups were the same, however in the experimental group Amon was down regulated. These are the results:

Lastly, in the course the data for the larvae experiment was collected only under blue light, so I used the same crossings and tested them under red light to use as a control.

When Andi tested his aPKC CRISPR knock-outs, he used the Tang torque meter setup, where the OMRs aren’t recorded. So I’m attempting to replicate his results and compare OMRs between groups.

It looks like I need to tweak the graph in some way to make the density plots show up. Obviously, N is too low to say anything yet, but I need to work on the plot.

All figures show the results for preference tests (salt/pure) conducted under red and blue light for different gal4-lines.

MB145B:

SS01716:

SS02180:

The first figure shows the results for the preference test (salt/pure) conducted with amon-RNAi only under red light.

For the next experiment I conducted the same preference test but with the split-gal4 line MB143B under red and blue light.

There had been some concerns about the optomotor display in the group evaluation sheets showing right-turning torque on the left side of the graph and vice versa. Also, the use of standard deviations seemed to blur differences between the experimental groups:

Because of these concerns, I have swapped the traces and used standard error of the means instead of standard deviations:

What do you think? Better or worse? Feedback very welcome!

The following picture shows the results for the conducted preference tests (pure/salt) for sNPF mutants. All Experiments were performed under red light.

Over the course, control and test lines were experimented with under ‘Joystick’. The test lines include 13:0273-Gal 4 line, SS56699 line, and TH-C-AD; TH-D-DBD line. The tests were done under red and yellow light conditions.

Red light conditions:

Yellow light conditions:

At long last, I got all the flies together that we need for sufficient statistical power. As the preliminary data had indicated, WTB flies don’t learn with the short training, while rut and rsh flies do just fine.

However, this may be due to genetic background effects, so we need to check the CRISPR mutants.

The flies without curly (straight wings) for the line NorpA;20xUAS-Chrimson were tested in T-maze in dark-light conditions. The tests were conducted on 23 November, 27th November, and 29th November 2023. 8 tests were done for each pink and green line; hence a total of 16 tests were conducted. A total of 273 flies and 233 flies were tested for pink line and green line respectively.