Rover/Sitter | .:lab.brembs.net:.

Self-learning, 8 minutes training, elav-Gal4>UAS-for-RNAi

on Monday, November 25th, 2024 11:47 | by Radostina Lyutova

It seems that panneuronal downregulation of the expression of the foraging gene impairs yow torque learning when flies were trained for 8 minutes.

Category: Operant learning, operant self-learning, PKG, Rover/Sitter | No Comments

Rover vs. Sitter self learning after 4 minutes training

on Monday, October 16th, 2023 10:39 | by Radostina Lyutova

Category: flight, Habit formation, Memory, Operant learning, operant self-learning, Rover/Sitter | No Comments

Food manipulation, starvation and density experiment

on Monday, March 1st, 2021 12:41 | by Simon Benmaamar

1. Food manipulation new

2. Starvation

Control: larvae were taken directly out of food and tested. Sucrose: larvae were allowed to roam freely for 2 hours in a 6 cm petri dish containing 350 µl of 0,2 M sucrose added to a piece of Kim wipe and then were tested. Starved: larvae were allowed to roam freely for 2 hours in a 6 cm petri dish containing 350 µl of 0,2 M dH20 added to a piece of Kim wipe and then were tested.

Distance by time plot

3. Density

Standard: about 15 female flies per vile. Low Density: about 5 female flies per vile. High Density: about 30 or more female flies per vile.

Category: Rover/Sitter | No Comments

Larval food manipulation evaluation

on Monday, February 8th, 2021 1:28 | by Simon Benmaamar

1. Food Patch

No significant difference between groups in Standard (wilcox.test, p =0.9442), Sugar (wilcox.test, p=0.3097) and Fat (wilcox.test, p-value = 0.06267).
Significant difference between Rover and Sitter in Standard_new (wilcox.test, p=0.01057), Carbohydrates (wilcox.test, p= 0.0002445), Starch (wilcox.test, p-value = 0.03431) and Yeast (wilcox.test, p = 0.001455)

2. Distance Tracking

Signifcant difference between Groups in Standard (t-test, p=0.0317), Sugar (t-test, p=0.0103), Fat (t-test, p=0.01823), Starch (t-test, p = 0.03895) and Yeast (t-test, p=0.001894).
No significant difference between Rover and Sitter in Standard_new (t-test, p =0.08108), Carbohydrates (t-test, p=0.09875),

Category: Rover/Sitter, Uncategorized | No Comments

Power analysis and food manipulation recipes

on Monday, January 25th, 2021 11:50 | by Simon Benmaamar

Power analysis sample size

Food manipulation recipes

Food recipes

Fly food protocol: 1 l of water was brought to a boil in a cooking pot using an induction cooker. Then 17 g of agar-agar dissolved in 350 ml of water was added and also brought to the boil. Diastatic malt extract and sugar beet sirup were added to the agar-water mixture. After that, polenta, brewer’s yeast and soy meal dissolved in 350 ml water were added to the pot. The mush was boiled for about 10 minutes while stirring continuously. Finally some water was added and after a short cooling down 6 g Nipagin dissolved in water was added. The mash was then filled into vials.

Nutrient distribution

a) Quantity distribution