With the focus on dopaminergic neurons, I conducted two experiments with two different driver lines to see how the naive gustatory behavior in Drosophila larvae is affected.

The first cross I tested was R58E02-Gal4 x UAS-ChR2-XXL, which gave me the following results:

As seen in the figures, there was no effect under blue light.

For the second experiment I crossed TH-Gal4 x UAS-ChR2-XXL with these results:

With this experiment, there was an effect on gustatory behavior as there is a clear significant difference between the experimental group and the control group.

The following plots show the results after I increased the salt concentration from 1,5M to 2,5M. It shows that the difference between the control groups and experimental group are not significant anymore.

MB417B -> α’/β’ap, α’/β’m, α/βs, α/βp
MB463B -> α’/β’ap
MB371B -> α/βp

If you missed it at the Göttingen NWG meeting, you can download our poster from here:

MB371B

MB417B

MB463B

MB063B

MB194B

MB010B-GAL4xUAS-CD8::GFP

MB152B-GAL4xUAS-CD8::GFP