MBON screen progress 11.05.2026

on Monday, May 11th, 2026 12:25 | by

PI-values unsubtracted:

Results from premature habit formation torque measurements of four drosophila crosses: MB399B, MB050B & MB027B gal4 driver lines crossed with UAS-Tetanus toxin lines and Wild type Berlin crossed with UAS-TnT as control. Which line is which is still unknown as the screen is incomplete and researcher must remain blind. It appears two crosses strongly form habits prematurely, one doesn’t and one is still on the borderline.

PI-values subtracted:

Same results as above except with pretest bias subtracted.

p-values:

p-values for the different groups
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Long form habit formation pooled data 16-20th & 23-27th of March 2026

on Monday, March 30th, 2026 12:07 | by

Performance index values for long sequence habit formation trial
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Short habit formation trial 11.03.2026

on Monday, March 16th, 2026 10:47 | by

Short form habit formation trial, indicating operant learning is abolished/delayed in the presence of external associative stimuli.
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Color learning 13.03.2026

on Monday, March 16th, 2026 10:38 | by

Results from first attempt at the habit formation trial. Strong positive preference in the beginning as well as low number of successful flies obscures the results. Reason for the disturbed OR at the end is that the offset for one fly was accidentally shifted while attempting to restart flight with a brush.
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Habit Formation Wildtype Test 02.03-06.03

on Tuesday, March 10th, 2026 10:45 | by

I wanted to try Habit formation with wildtype and tested 4 wheels of flies (48) over the last week. Unfortunately, I did a mistake when setting the timeslots file for right torque so it had an additional testing period at P13. So analyzed the left and right individually:

For Punishment on Left Torque:

Optomotor traces (right/left) at start of experiment:

Performance Index box&dotplot without notches:

For Punishment on Right Torque:

Optomotor traces (right/left) at start of experiment:

Performance Index box&dotplot without notches:

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Torque measurements 21.02 & 22.02

on Monday, February 23rd, 2026 12:21 | by

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Control Experiments for Driver Line Expression

on Monday, February 16th, 2026 12:36 | by

Giant fiber Gal4 driver (BDSC 79602), green channel shows giant fiber neurons, magenta channel shows background staining with nc82:

Giant fiber neurons on Codex BANC v626 dataset:

b1 mn driver (SS40980, also targets iii1 mn, iii3 mn):

Janelia image for SS40980:

b3 mn driver (SS48311, also targets hg1 MN, XBI002, WBI009):

Janelia image for SS48311:

b3 mn driver (SS98650):

Janelia image for SS98650:

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Torque measurements 12.02.2026

on Friday, February 13th, 2026 12:38 | by

Torque results 12.02.2026, learning is abolished and many of the flies died. Think improper feeding is to blame. Granulated sugar had become encrusted and possibly inaccessible to the flies, potentially resulting in starvation. In the future, I’ll resolve to use less sugar and to clean more frequently to prevent this kind of buildup.
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Torque measurements 10.02.2026

on Friday, February 13th, 2026 12:31 | by

Results for 10.02.2026, 24 flies, 7 completed trial. Main source of failure was flies stopping flight and refusing to resume. Had some difficulty getting flightless torque on the unpunished domain.

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Torque measurements 2.2. – 6.2

on Monday, February 9th, 2026 12:41 | by

Results from 3.2. with the laser at the lowest intensity
Results from 5.2. with the laser at an increased intensity
Results from 6.2. with the laser at about 1/3 intensity
Combined results from last week
Breakdown of results
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