I wanted to try Habit formation with wildtype and tested 4 wheels of flies (48) over the last week. Unfortunately, I did a mistake when setting the timeslots file for right torque so it had an additional testing period at P13. So analyzed the left and right individually:

For Punishment on Left Torque:

Optomotor traces (right/left) at start of experiment:

Performance Index box&dotplot without notches:

For Punishment on Right Torque:

Optomotor traces (right/left) at start of experiment:

Performance Index box&dotplot without notches:

Giant fiber Gal4 driver (BDSC 79602), green channel shows giant fiber neurons, magenta channel shows background staining with nc82:

Giant fiber neurons on Codex BANC v626 dataset:

b1 mn driver (SS40980, also targets iii1 mn, iii3 mn):

Janelia image for SS40980:

b3 mn driver (SS48311, also targets hg1 MN, XBI002, WBI009):

Janelia image for SS48311:

b3 mn driver (SS98650):

Janelia image for SS98650:

Habit formation circuit in Drosophila Melanogaster

Fridrik Kjartansson*, Ipek Subay*, Radostina Lyutova, Björn Brembs
University of Regensburg, Zoology – Neurogenetics, Regensburg, Germany
*These authors contributed equally.

Classical learning forms association between a novel external stimulus and a
consequence of innate value. Conversely, operant learning associates animals
own behavior to such consequences; eventually habits form which ensure fast
and efficient behaviors. These forms of learning can reciprocally interact with
each other, with classical learning prioritized. A previous study from our group
demonstrated a scenario where in the case of two types of learning paradigms
introduced simultaneously in Drosophila, the classical stimulus was dominant.
However, when the training period was doubled, learning shifted to operant,
allowing habit formation in flies. Moreover, when the prominent associative
learning center, Mushroom Bodies (MB), are genetically silenced, extended
training becomes unnecessary and flies show premature habit formation.
Another study screened Mushroom Body Output Neurons (MBONs), which relay
signals from the MB and found that silencing MBON-02 specifically results in
premature habit formation suggesting MBON-02 acts as final integration center
for the selection between learning types. In this study, we utilize the power of
trans-tango and retro-tango techniques to identify the synaptic partners of
MBON-02 along with validating our results with existing connectomics datasets to
map the whole circuit that regulates the switch between these two types of
associative learning. Through behavioral testing using tethered flight, we will also
narrow down which types of MB cells, called Kenyon cells (KC) , are necessary for
the dominance of classical stimuli in such trials; furthermore, we’ll target
dopaminergic PAM neurons which form feedback loops with MBONs and KC to
elucidate their involvement.