I tested more wtb, this time with the switch mode protocol. Here are the results:

With the modified laser settings I was able to test 3 more wtb. Here are the results:

–> yaw torque

Before collecting the actual data I make sure that the flight simulator in the basement does its thing ;) Here are the test runs with wtb flies for different protocols:

—>> yaw torque

–>> switch mode

–>> habit formation

Below you can find the data I collected from the offspring flies:

-> offspring from trained parents

-> offspring from untrained parents

(I left out data from flies that showed negative preference during two training periods in a row)

Below you find the data from my experimental rounds A and B:

-> learning scores of the parental flies from experimental round A and B

-> learning scores of the trained parent´s offspring only from experimental round A

-> learning scores of the untrained parent´s offspring only from experimental round A

The results confuse me a lot and I am happy to discuss reasons :) However the offspring of the round-B will be ready for testing by the end of this week so there is still some data to collect…

The offspring of my first experimental fly cohort finally hatched! Below you find a few first pre-tests I ran last week :)

First, here are the results of a quick test to see if the offspring shows a preference for the parentally trained side after the first training period:

After that I played around with the laser a little bit to find the learning threshhold. I set the laser on 2,6V but the results I got look a bit weird:

-> untrained wtb flies

-> offspring of trained wtb flies

I´m optimistic however there is still a lot of work to do…

MB417B -> α’/β’ap, α’/β’m, α/βs, α/βp
MB463B -> α’/β’ap
MB371B -> α/βp

Knockout of Kibra in adult flies.

Knockout of Kibra in adult flies.

Results from day1 (not included)