Salt (1.5 M) avoidance tests in larvae (Dop2R) under red and blue light (N=15)

on Sunday, March 22nd, 2026 1:39 | by

Significant p-values:

Group A (RL <-> BL): 0.013

Group B (RL <-> BL): 0.044

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T4/T5 lines test crossings

on Monday, March 16th, 2026 12:04 | by

Different T4/T5 driver lines are crossed with different effector lines to assess the viability of the F1 progeny

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Category: crosses | No Comments

TH Driver lines larval expression verification

on Monday, March 16th, 2026 10:58 | by

TH-D’-Gal4>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

NP1568-Gal4>UAS-mCD8::GFP (adult brains); aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

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Short habit formation trial 11.03.2026

on Monday, March 16th, 2026 10:47 | by

Short form habit formation trial, indicating operant learning is abolished/delayed in the presence of external associative stimuli.
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Color learning 13.03.2026

on Monday, March 16th, 2026 10:38 | by

Results from first attempt at the habit formation trial. Strong positive preference in the beginning as well as low number of successful flies obscures the results. Reason for the disturbed OR at the end is that the offset for one fly was accidentally shifted while attempting to restart flight with a brush.
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Salt (1.5 M) avoidance tests in larvae (Dop2R) under red and blue light

on Sunday, March 15th, 2026 4:49 | by

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TH Driver lines larval expression verification

on Tuesday, March 10th, 2026 12:04 | by

NP1568-Gal4/CyO>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

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Habit Formation Wildtype Test 02.03-06.03

on Tuesday, March 10th, 2026 10:45 | by

I wanted to try Habit formation with wildtype and tested 4 wheels of flies (48) over the last week. Unfortunately, I did a mistake when setting the timeslots file for right torque so it had an additional testing period at P13. So analyzed the left and right individually:

For Punishment on Left Torque:

Optomotor traces (right/left) at start of experiment:

Performance Index box&dotplot without notches:

For Punishment on Right Torque:

Optomotor traces (right/left) at start of experiment:

Performance Index box&dotplot without notches:

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Last stressor for pre-test: sleep deprivation

on Tuesday, March 10th, 2026 10:38 | by

stressor: Sleep deprivation (24h, room temperature)

POS_CTRL: control group

Gr28bd+TrpA1(sleep): experimental group

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Salt (1.5 M) avoidance tests in larvae (DANc1) under blue and red light (N = 20)

on Sunday, March 8th, 2026 5:55 | by

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