MBON screen progress 18.05.2026

on Monday, May 18th, 2026 12:32 | by

PI- values for world learning torque experiments. As usual the flies were tethered and subjected to adverse stimuli by laser dependent on torque domain, which also controlled arena color. Flies that show positive PI at the end indicate premature habit formation as WT flies don’t learn under these conditions in this time frame.
Same as above except the pretest preference has been subtraced
Current p-values for all the tested groups. Should be 25-30 flies at trial end.

Trans-tango repeat from previous week

on Monday, May 18th, 2026 12:21 | by

I was not able get any trans-tango signal from my reciprocal MB399B crosses so I repeated the experiment with anti-RFP antibody instead of anti-HA antibody

77124xMB399B (MBON02 reciprocal cross) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-RFP (2nd: anti-rat 555), nc82 (2nd: anti-mouse 650)

MBON02 trans-tango images from Kaun Lab as reference:

MBON screen progress 11.05.2026

on Monday, May 11th, 2026 12:25 | by

Results from premature habit formation torque measurements of four drosophila crosses: MB399B, MB050B & MB027B gal4 driver lines crossed with UAS-Tetanus toxin lines and Wild type Berlin crossed with UAS-TnT as control. Which line is which is still unknown as the screen is incomplete and researcher must remain blind. It appears two crosses strongly form habits prematurely, one doesn’t and one is still on the borderline.

PI-values subtracted:

Same results as above except with pretest bias subtracted.

p-values:

p-values for the different groups

NWG Poster on MBON-02 regulating habit formation

on Thursday, March 23rd, 2023 12:18 | by

If you missed it at the Göttingen NWG meeting, you can download our poster from here:

MBON screen verification

on Tuesday, December 21st, 2021 9:04 | by

Learning performance

Learning Score Statistics

World- vs. self-learning, MBON screen verification, Laser 3.06V

on Monday, November 29th, 2021 1:14 | by