Anatomy | .:lab.brembs.net:.

Results for Dop1R1-RNAi, Amon-RNAi and the course data

on Tuesday, May 21st, 2024 8:11 | by Maja Achatz

Firstly, I did an experiment where Dop1R1 was knocked down via RNAi. The following shows the results:

For the next experiment, the controls groups were the same, however in the experimental group Amon was down regulated. These are the results:

Lastly, in the course the data for the larvae experiment was collected only under blue light, so I used the same crossings and tested them under red light to use as a control.

Category: Kenyon cells, Larve, Mushroom Body, Optogenetics, Uncategorized | No Comments

Results for MB+DANs and MB054B

on Monday, September 25th, 2023 11:07 | by Maja Achatz

The first experiment tested the activation of KCs with simultaneous ablation of pPAM which showed the following results:

Green: UAS-ChR2-XXL; LexAop-rpr x 58E02-LexA; H24-Gal4

Lightgrey: UAS-ChR2-XXL; LexAop-rpr x H24-Gal4

Darkgrey: UAS-ChR2-XXL x 58E02-LexA; H24-Gal4

The next experiment was conducted with the split-Gal4 line MB054B (DAN-f1/DAN-g1) and showed the following:

Category: Kenyon cells, Larve, Mushroom Body, Optogenetics | No Comments

Verification TH-C-AD;TH-D-DBD

on Monday, September 11th, 2023 9:52 | by Luisa Guyton

**Confocal images with anti-GFP and anti-Brp staining of TH-C-AD;TH-D-DBD > mCD::GFP fly brains:** A: 4 PPM 2 DANs per hemisphere marked with blue circles, three clusters of Kenyon cell bodies per hemisphere marked with white arrows. B: Five visible cell bodies of unidentified neurons marked with a blue circle in the left hemisphere. The neurons project into the lobula plate and the medulla. Strong fluorescence of cell bodies of the Kenyon cells projecting into the Mushroom bodies is visible. C: Unidentified neurons projecting into structures outside of the optic lobes especially the ventral lateral protocerebrum.

Category: Anatomy, DAN, genetics, Kenyon cells, Optogenetics | No Comments

Line verification SS56699

on Tuesday, August 29th, 2023 7:50 | by Luisa Guyton

After dissecting brains from the GAL4 driver line SS56699 with GFP staining and finding no fluorescence, I dissected them again and the immunohistochemical staining showed fluorescence. To check that the correct neurons were stained, I compared the images with the image in the paper by Hulse et al (2021; https://doi.org/10.7554/eLife.66039) . The three PPL1 dopaminergic dorsal fan-shaped body tangential neurons per hemisphere are stained, but there are some additional unidentified neurons (presumably PPM1 neruons) visible.

Hulse, B. K., Haberkern, H., Franconville, R., Turner-Evans, D., Takemura, S.-y., Wolff, T., Noorman, M., Dreher, M., Dan, C., Parekh, R., Hermundstad, A. M., Rubin, G. M., & Jayaraman, V. (2021). A connectome of the Drosophila central complex reveals network motifs suitable for flexible navigation and context-dependent action selection. eLife, 10, e66039. https://doi.org/10.7554/eLife.66039

Category: Anatomy, genetics | No Comments