MBON02 trans-tango image from Kaun Lab. Left image shows trans-tango signal in red, right image shows overlay of all channels (green for MBON02, red for trans-tango, blue for nc82):

My MBON02 trans-tango images stained with anti-HA instead of anti-RFP. Flies generated by crossing trans-tango mkII line (BDSC: 95317) with MB399B (Brembslab stock number: 319). Left shows trans-tango signal in green, right shows overlay of al channels (green for trans-tango, blue for MBON02, red for nc82):

Janelia Flylight image of MB399B with 20XUAS-CsChrimson-mVenus trafficked in attP18 reporter:

Staining done by anti-GFP (MBON02), anti-RFP (post-synaptic cells), nc82 (for background stainingm not shown here). MBON02 cells are clearly visible on green channel but there is no clear cell signal on red channel (MBON02 in green channel, postsynaptic cells in red channel).

Here is the trans-tango image of MBON02 (postsynaptic cells of MBON02) from Kaun Lab:

Retro-Tango of b3 mns look worse, nearly no cell signal both in green (b3 mn) and red channels (b3 mn presynaptic neurons)

Firstly, I did an experiment where Dop1R1 was knocked down via RNAi. The following shows the results:

For the next experiment, the controls groups were the same, however in the experimental group Amon was down regulated. These are the results:

Lastly, in the course the data for the larvae experiment was collected only under blue light, so I used the same crossings and tested them under red light to use as a control.

The first experiment tested the activation of KCs with simultaneous ablation of pPAM which showed the following results:

Green: UAS-ChR2-XXL; LexAop-rpr x 58E02-LexA; H24-Gal4

Lightgrey: UAS-ChR2-XXL; LexAop-rpr x H24-Gal4

Darkgrey: UAS-ChR2-XXL x 58E02-LexA; H24-Gal4

The next experiment was conducted with the split-Gal4 line MB054B (DAN-f1/DAN-g1) and showed the following: