Anatomy comparison D42/FoxP
on Monday, May 23rd, 2022 1:41 | by Amelie Hauser
Category: Anatomy | No Comments
Anatomy comparison C380/FoxP
on Monday, May 23rd, 2022 1:38 | by Amelie Hauser
Category: Anatomy | No Comments
Anatomy comparison aPKC/FoxP
on Monday, May 23rd, 2022 1:33 | by Amelie Hauser
Category: Anatomy | No Comments
Anatomy comparison
on Monday, March 28th, 2022 12:06 | by Andreas Ehweiner
Compairison of the previous shown expression pattern (FoxP: red, D42: green) with the figures from Maniates-Selvin et. al. 2020.
Top row: dorsal view
Bottom row: dorsal view tilted to the right
![](https://lab.brembs.net/wp/wp-content/uploads/2022/03/VCND42FoxPOverlap-1024x980.png)
Category: Anatomy, Foxp | No Comments
Overlap FoxP – motor neuros/aPKC
on Monday, March 14th, 2022 11:40 | by Andreas Ehweiner
Category: Anatomy, Foxp, PKC, PKC_localisation, Uncategorized | No Comments
Possible canidate for overlap
on Monday, January 10th, 2022 1:36 | by Andreas Ehweiner
By comparing the FoxP expression pattern with canidate neurons, VGlut-F-400630 could be an interaction point of FoxP-neurons with the MB.
![](https://lab.brembs.net/wp/wp-content/uploads/2022/01/VGlut-F-400630comp-1024x877.png)
Category: Anatomy, Foxp | No Comments
Colocalization of FoxP
on Monday, August 30th, 2021 12:48 | by Andreas Ehweiner
Cross of FoxPLexA (red) with diffrent GFP-Lines (green)
GMR65A06
![](https://lab.brembs.net/wp/wp-content/uploads/2021/08/39330.65H05GfpFoxPLexA.jpg)
GMR20H05
![](https://lab.brembs.net/wp/wp-content/uploads/2021/08/47896.20H05Gal4FoxPLexA2-1-1024x1024.jpg)
GMR11F02 (dose not overlap)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/08/49828.11F02.GfpFoxPLexA2-1.jpg)
GMR52B10 not checked yet
![](https://lab.brembs.net/wp/wp-content/uploads/2021/08/38820.52B10GfpFoxPLexA.jpg)
aPKC (must be repeated)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/08/aPKC1-1024x1024.jpg)
Category: Anatomy, Foxp, Uncategorized | No Comments
MBON Expression pattern verification
on Monday, July 12th, 2021 2:03 | by Radostina Lyutova
MB027B-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/11/MB027B_4.1_aGFP_aBrp-300x300.png)
MB082C-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/11/MB082B_3.1_aGFP_aBrp-300x300.png)
MB093C-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/11/MB093C_4.1_aGFP_aBrp-300x300.png)
MB112C-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/11/MB112C_8.1_aGFP_aBrp-300x300.png)
MB242A-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/11/MB242A_1.1_aGFP_aBrp-300x300.png)
MB549C-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/11/MB549C_5.1_aGFP_aBrp-300x300.png)
MB018B-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/11/MB018B_7.1_aGFP_aBrpRGB-300x300.png)
MB077B-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/10/MB077B_3.1_aGFP_aBrpRGB-300x300.png)
MB051B-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/10/MB051B_8.1_aGFP_aBrpRGB-300x300.png)
MB543B-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/09/MB543B_2.1_aGFP_aBrpRGB-364x300.png)
MB542B-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/09/MB542B_3.1_aGFP_aBrpRGB-300x300.png)
MB083C-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/09/MB083B_1.1_aGFP_aBrpRGB-300x300.png)
MB050B-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/09/MB050B_1.1_aGFP_aBrpRGB-300x300.png)
MB057B-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/07/MB057C_1.1_aGFP_aBrpRGB-300x300.png)
MB085C-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/07/MB085C_5.1_aGFP_aBrpRGB-300x300.png)
MB110C-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/07/MB110C_6.1_aGFP_aBrpRGB-300x300.png)
MB310C-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/07/MB310C_4.1_aGFP_aBrpRGB-300x300.png)
MB002B-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/07/MB002B_2.1_aGFP_aBrpRGB-300x300.png)
MB011B-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/07/MB011B_4.1_aGFP_aBrpRGB-300x300.png)
MB210B-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/07/MB210B_2.1_aGFP_aBrpRGB-300x300.png)
MB399B-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/07/MB399B-300x300.png)
MB298B-GAL4/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/07/MB298B_1.1_aGFP_aBrpRGB-300x300.png)
MB433B-GAL/UAS-CD8::GFP (aGFP, aBrp)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/07/MB433B-300x300.png)
Category: Anatomy, MBON | No Comments
Fas II Immunohistochemistry Protocol
on Tuesday, April 13th, 2021 10:57 | by Parva Nasimi
Fluorescence labeling and microscopy of adult Drosophila mushroom body:
1. Dissection: The brains are removed in cold Phosphate-buffered saline (1X PBS, pH 7.4).
2. Fixation: Tissues are fixed for 20 min in 4% paraformaldehyde (Merck, Germany) in PBST on an orbital shaker at room temperature.
3. Washing: Three quick (each 30 sec) and three long washing steps (each 15 min) and once for 2h with PBST on an orbital shaker at room temperature.
4. Blocking: 5% normal goat serum in PBST are used as a blocking solution for 30 min on an orbital shaker at room temperature.
5. Primary antibody: Brains were incubated for 2 nights in mouse anti-fasciclin II monoclonal antibody (Fas2; 1D4, Developmental Studies Hybridoma Bank, Iowa City, IA) diluted 1:20 in 5% NGS and at 4°C on an orbital shaker.
6. Washing: Brains are washed for 3×30 sec and 4×15 min with PBST at room temperature with orbital shaker.
7. Secondary antibody: Brains are incubated with Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Cat#A-11032, ThermoFisher Scientific) at a 1:200 dilution in 5% NGS and PBST for 24hr at room temperature on an orbital shaker in the dark.
8. Washing: Brains are washed for 3×30 sec and 4×15 min with PBST at room temperature with orbital shaker.
9. Mounting: Brains are placed on glass microscope slides and mounted in antifade mounting medium Vectashield® (Vector Laboratories, Burlingame, CA).
10. Slides are stored at 4°C in the dark until microscopy.
Points:
– Use 1X PBS, pH 7.4, 0.3% Triton X-100 (PBST) in step 2.
– Use 10X PBS, pH 7.4, 0.3% Triton X-100 (PBST) in steps 3 to 8.
– Keep the dish containing brains in 1X PBS on ice and fix them within one hour of dissection.
– Transfer the brains using a100 μl pipette.
– Rinse the empty pipette tips twice with PBST (prevent tissues from sticking to the plastic tips).
Category: Anatomy, lab.brembs.net | No Comments
FoxP in the fan-shape body
on Friday, January 15th, 2021 12:10 | by Andreas Ehweiner
Control of general FoxP expression patter (line 104y)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/01/82014-foxponly-1024x1024.jpg)
Expression of 104y (green) and FoxP (red)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/01/81014.2-1024x1024.jpg)
There seems to be no colocalisation of 104y and FoxP.
![](https://lab.brembs.net/wp/wp-content/uploads/2021/01/81014-fb-1024x1024.jpg)
Expression of 121y (green) and FoxP (red)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/01/30815overlap-1024x1024.jpg)
Overlap of 121y GFP expression with FoxP expression is the fan-shape body.
![](https://lab.brembs.net/wp/wp-content/uploads/2021/01/30815fb2-1024x1024.jpg)
Problems with line c205, no GFP expression.
![](https://lab.brembs.net/wp/wp-content/uploads/2021/01/30826.2-1024x1024.jpg)
Category: Anatomy, Foxp | No Comments