Anatomy | .:lab.brembs.net:.

MBON screen progress 26.05.2026

on Tuesday, May 26th, 2026 9:05 | by Fridrik Kjartansson

Category: flight, Habit formation, MBON, MBON, Operant learning, Operant reinforcment, operant self-learning, World learning | No Comments

TH Driver lines larval expression verification

on Friday, May 22nd, 2026 12:57 | by Radostina Lyutova

MB299B>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

Category: Anatomy, Biogenic Amines, Larvae, TH | No Comments

MBON screen progress 18.05.2026

on Monday, May 18th, 2026 12:32 | by Fridrik Kjartansson

PI- values for world learning torque experiments. As usual the flies were tethered and subjected to adverse stimuli by laser dependent on torque domain, which also controlled arena color. Flies that show positive PI at the end indicate premature habit formation as WT flies don’t learn under these conditions in this time frame.

Same as above except the pretest preference has been subtraced

Current p-values for all the tested groups. Should be 25-30 flies at trial end.

Category: crosses, Habit formation, MBON, MBON, MBON, Memory, Operant learning, Operant reinforcment, operant self-learning, World learning | No Comments

Trans-tango repeat from previous week

on Monday, May 18th, 2026 12:21 | by Ipek Subay

I was not able get any trans-tango signal from my reciprocal MB399B crosses so I repeated the experiment with anti-RFP antibody instead of anti-HA antibody

77124xMB399B (MBON02 reciprocal cross) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-RFP (2nd: anti-rat 555), nc82 (2nd: anti-mouse 650)

MBON02 trans-tango images from Kaun Lab as reference:

Category: Anatomy, Habit formation, MBON, MBON | No Comments

Tango imaging

on Monday, May 11th, 2026 12:32 | by Ipek Subay

99661xSS98650 (retro tango imaging for b3 mn) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-RFP (2n: anti-rat 555), nc82 (2nd: anti-mouse 650)

GFP channel:

somehow my antibodies decided to work on retro tango flies even though I did not change anything in the protocol

retro-tango channel:

b3 presynaptic neurons from BANC dataset:

99661xSS48311 (b3 mn) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-RFP (2n: anti-rat 555), nc82 (2nd: anti-mouse 650)

GFP:

Retro-tango:

99661xSS40980 (b1 mn) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-RFP (2n: anti-rat 555), nc82 (2nd: anti-mouse 650)

GFP:

retro-tango:

b1 presynaptic neurons from BANC dataset:

77124xMB399B (MBON02 reciprocal cross) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-HA (2n: anti-rat 555), nc82 (2nd: anti-mouse 650)

GFP: