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MBON screen progress 19.06.2026

on Monday, June 22nd, 2026 12:15 | by Fridrik Kjartansson

MBON screen of MB399 for MBON02, MB050B for MBON15, MB027B for MBON17 all crossed with TnTe and WTB x TnTe for the negative control. Flies are tethered in torque meter and one turning domain is punished with a hot laser invisible to the fly. Each turning domain is also associated with an arena color. The lights are turned off at the last two periods. Wild type flies fail to learn to avoid the punished domain after the training periods in these conditions. A positive PI-score in the 12th period indicates that the fly learned.

Category: flight, Habit formation, MBON, MBON, Memory, Mushroom Body, Operant learning, Operant reinforcment, operant self-learning, World learning | No Comments

MBON Screen progress 01.06.2026

on Monday, June 1st, 2026 12:59 | by Fridrik Kjartansson

Category: flight, Habit formation, MBON, MBON, MBON, Operant learning, Operant reinforcment, operant self-learning, World learning | No Comments

MBON screen progress 26.05.2026

on Tuesday, May 26th, 2026 9:05 | by Fridrik Kjartansson

Category: flight, Habit formation, MBON, MBON, Operant learning, Operant reinforcment, operant self-learning, World learning | No Comments

MBON screen progress 18.05.2026

on Monday, May 18th, 2026 12:32 | by Fridrik Kjartansson

PI- values for world learning torque experiments. As usual the flies were tethered and subjected to adverse stimuli by laser dependent on torque domain, which also controlled arena color. Flies that show positive PI at the end indicate premature habit formation as WT flies don’t learn under these conditions in this time frame.

Same as above except the pretest preference has been subtraced

Current p-values for all the tested groups. Should be 25-30 flies at trial end.

Category: crosses, Habit formation, MBON, MBON, MBON, Memory, Operant learning, Operant reinforcment, operant self-learning, World learning | No Comments

Trans-tango repeat from previous week

on Monday, May 18th, 2026 12:21 | by Ipek Subay

I was not able get any trans-tango signal from my reciprocal MB399B crosses so I repeated the experiment with anti-RFP antibody instead of anti-HA antibody

77124xMB399B (MBON02 reciprocal cross) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-RFP (2nd: anti-rat 555), nc82 (2nd: anti-mouse 650)

MBON02 trans-tango images from Kaun Lab as reference:

Category: Anatomy, Habit formation, MBON, MBON | No Comments

Tango imaging

on Monday, May 11th, 2026 12:32 | by Ipek Subay

99661xSS98650 (retro tango imaging for b3 mn) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-RFP (2n: anti-rat 555), nc82 (2nd: anti-mouse 650)

GFP channel:

somehow my antibodies decided to work on retro tango flies even though I did not change anything in the protocol

retro-tango channel:

b3 presynaptic neurons from BANC dataset:

99661xSS48311 (b3 mn) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-RFP (2n: anti-rat 555), nc82 (2nd: anti-mouse 650)

GFP:

Retro-tango:

99661xSS40980 (b1 mn) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-RFP (2n: anti-rat 555), nc82 (2nd: anti-mouse 650)

GFP:

retro-tango:

b1 presynaptic neurons from BANC dataset:

77124xMB399B (MBON02 reciprocal cross) stained with rabbit anti-GFP (2nd: anti-rabbit 488), rat anti-HA (2n: anti-rat 555), nc82 (2nd: anti-mouse 650)

GFP:

trans-tango:

trans-tango maximum intensity projection:

Category: Habit formation, MBON, Motor neurons | No Comments

MBON screen progress 11.05.2026

on Monday, May 11th, 2026 12:25 | by Fridrik Kjartansson

PI-values unsubtracted:

Results from premature habit formation torque measurements of four *drosophila* crosses: MB399B, MB050B & MB027B gal4 driver lines crossed with UAS-Tetanus toxin lines and Wild type Berlin crossed with UAS-TnT as control. Which line is which is still unknown as the screen is incomplete and researcher must remain blind. It appears two crosses strongly form habits prematurely, one doesn’t and one is still on the borderline.

PI-values subtracted:

Same results as above except with pretest bias subtracted.

p-values:

Category: crosses, flight, Habit formation, MBON, MBON, MBON, Operant learning, operant self-learning, World learning | No Comments

MBON02 Trans-tango images

on Monday, December 15th, 2025 12:37 | by Ipek Subay

MBON02 trans-tango image from Kaun Lab. Left image shows trans-tango signal in red, right image shows overlay of all channels (green for MBON02, red for trans-tango, blue for nc82):

My MBON02 trans-tango images stained with anti-HA instead of anti-RFP. Flies generated by crossing trans-tango mkII line (BDSC: 95317) with MB399B (Brembslab stock number: 319). Left shows trans-tango signal in green, right shows overlay of al channels (green for trans-tango, blue for MBON02, red for nc82):

Janelia Flylight image of MB399B with 20XUAS-CsChrimson-mVenus trafficked in attP18 reporter:

Category: Habit formation, MBON, MBON, trans-tango | No Comments

Confocal Images Trans-Tango, Retro-Tango

on Monday, December 8th, 2025 12:44 | by Ipek Subay

Staining done by anti-GFP (MBON02), anti-RFP (post-synaptic cells), nc82 (for background stainingm not shown here). MBON02 cells are clearly visible on green channel but there is no clear cell signal on red channel (MBON02 in green channel, postsynaptic cells in red channel).