First evaluation of the Rover/Sitter larvae

on Monday, November 2nd, 2020 11:17 | by

I have tested 514 larvae from the forR/forS Control Group and 480 larvae from the forR/forS Crowded Group. Here are the results:

Here you can see the first Control Group I have tested 2 weeks ago. In total 203 larvae were tested (ForS: 101, ForR 102). Rover larvae left the food patch moren often, however there is only a small difference between the Groups: 4,95 percent of the sitter larvae and 8,82 percent of the rover larvae left the first food patch after 20 minutes.
Here you can see the second Control Group, which had a lower density compared to the first Control Group. 311 larvae were tested (155 sitter, 156 rover). Again rover larvae left the food patch more often than sitter larvae, however there is only a small difference between the groups. 14,19 percent of the sitter larvae and 19,87 percent of the rover larvae left the first patch. Interestingly both groups seemed to leave the first food patch more often compared to the first control group with a little bit higher density.
Here you can see the entire ForS/ForR Control Group (256 sitter larvae, 258 rover larvae). 10,55 percent of the sitter larvae and 15,50 percent of the rover larvae left the first food patch after 20 minutes.
Here you can see the entire ForR/Fors Group under crowded conditions. 236 sitter larvae and 244 rover larvae were tested. 9,75 percent of the sitter larvae and 5,33 percent of the rover larvae left the food patch A after 20 minutes.
Here the entire rover/sitter Control Group and Crowded Group were compared. 13,04 percent of the rover and sitter larvae from the Control Group left the first food patch whereas only 7,50 percent of the entire larvae from the Crowded Group left the first patch. So at least there seems to be an effect of the forage competition/food quality on the foraging behaviour.
This effect seems to come nearly exclusively from the forR Group.

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New review of the shiming-setup

on Monday, October 26th, 2020 1:56 | by

Recent wtb test

There often seems to be a strong preference for a side. The laser was off.

Fly had a strong pref for left side, laser was off

Flies often seem to have a strong preferenc for a side. In the imiage below the top fly was punished for left torque and survived, the fly below was punished for the right torque side and died.

Trails with drift

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Ato x Cas9FoxP

on Monday, August 31st, 2020 9:16 | by

Knockout of FoxP in Ato.

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Progress week 26-28

on Monday, July 13th, 2020 1:39 | by

Updates in DTS code

Exp_ts ElavG4;TubGal80Download

Refreshing dissection skills

Tdc2-Gal4 expression in adult brain
Actin-GFP expression in adult brain

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Progress for week 25:

on Monday, June 22nd, 2020 1:58 | by

DTS coding
-Added progressbar for data validation
-Updated the progress bar (see figure 1)
-Fixed bug with wrong sample size (see figure 2)
-Fixed bug with unorganized barplots (see figure 2)

Figure 1. Updated progress bar
Figure 2. Correct sample size and restructured barplot


Exp always to the right:
plotOMparams <- plotOMparams[order(plotOMparams$desc),]
plotOMparams$group <- factor(plotOMparams$group, levels=paste(unique(plotOMparams$group)))

Samplesize fix:
samplesizes.annotate(boxes, as.numeric(table(plotOMparams$desc)))

Progressbar:
progress <- c(round(l(100/(length(xml_list)))),round(flycount(100/(totalflies))))

Rescreening:
-Finished rescreening last Thursday. Started to evaluate the new data

Optomotor platform: Ran a few more tests to confirm that the machine was still working, it is. I also adjusted the 0 line so that it is at 0, by readjusting the “zero line” screw. Looks much better now but it is still not perfectly at 0. A difference 0.1 on the computer screen translates to 100 in the evaluation chart.

Optomotor platform:
Ran a few more tests to confirm that the 0 line is always at 0. Readjusted the “zero line” screw. Looks much better now. It is still not perfectly at 0 but a difference of 0.1 in the chart translates to 100 in the evaluation graph.

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Recent experiments

on Monday, June 22nd, 2020 1:52 | by

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color change

on Monday, May 11th, 2020 11:38 | by

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FoxP cond

on Monday, April 20th, 2020 2:43 | by

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New problems with the set-up

on Wednesday, April 15th, 2020 8:46 | by

The measuring device needed repairing, after aperently some glued part got loosloosened and produced no signal anymore. The divice is now producing a signal again, but it is shifted.

The direction of the fly seems to be the main thing. Same fly faceing to the laser (left) and away (right)

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Updates on optomotor response software

on Friday, March 20th, 2020 3:19 | by

  • Added a timer
  • Previous version generated faulty xml files. This bug has been squashed (see image below)
  • Display window of the software now also prints which direction the striped arena turns
The highlighted red areas are the ones that caused issues. Contingency has also been added to the xml file from the optomotor response (not shown). This did not cause any errors when running the DTS analysis Rscript but I thought it looked nicer having it there.
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