Anders Eriksson

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Optomotor response works consistently with old platform

on Friday, March 20th, 2020 3:23

The old platform works a lot more consistently compared to the new one. Unless major improvements are seen I do not see any reason to update it.

Updates on optomotor response software

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  • Added a timer
  • Previous version generated faulty xml files. This bug has been squashed (see image below)
  • Display window of the software now also prints which direction the striped arena turns
The highlighted red areas are the ones that caused issues. Contingency has also been added to the xml file from the optomotor response (not shown). This did not cause any errors when running the DTS analysis Rscript but I thought it looked nicer having it there.

New optomotor platform not yet optimized

on Monday, March 16th, 2020 1:42

A new platform have been designed for the optomotor response. It is placed on a pivot with the axis attached on the back. In contrast to the previous design witch only monitored movement in the left/right direction, the new design in theory allows the fly to rotate the platform.

Optomotor evaluation

on Wednesday, February 19th, 2020 1:51

Optogenetic T-maze

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The figure below contains the separate data from both Emer, and Ferruh/Kader. Both data sets have been merged too.

Fly linesample size
Gr28 ATR60
Gr28no56
W111835
THD’-ATR35
THD’no36
THD1no35
THD1-ATR37

Dwelling time

on Monday, February 3rd, 2020 1:41

The schematic drawing is an example. The method that is currently used to calculate the dwelling time is excluding times that are 50 ms. Each dwelling time is getting an additional 50ms to compensate for the transitional time loss (dashed line in schematic drawing). The chart on the right side is the total time for each period when adding negative and positive dwelling times.

Category: R code | No Comments

T-maze screen with yellow light

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Fluorescence histogram

on Monday, January 27th, 2020 1:48

Proof of concept. I was playing around with ImageJ and found that there is a built in function already that does analyze the fluorescence intensity. I am just not sure exactly what it tells us. This is only as far as I have gotten yet.
The image below is the one I used.

Dwelling Time

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The code generates the dwelling time for each period as well as the median dwelling time for each period. The code has also been implemented on grouped data but it only generates graphs for one of the groups, not both. Currently investigating why

Just gloating here

Valence and dopamingergic neurons

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I have been sorting and feeding flies ATR (in total about 7400 flies divided between 170 vials). Emer has been conducting the experiments during the previous week continuing this week.
Including the flies from today we have a total of 172 experiments divided between 7 experimental groups. Including the data from the Turkish students it is well over 230.

I looked at the control experiments and they look as expected. Perhaps even better than what the Turkish students had.