Results from week 15-20.3.2026 of the long form habit formation trial. Only 6 flies out of 24 managed to successfully complete the trial. Avoidance was improved by increasing laser voltage.
Results from first attempt at the habit formation trial. Strong positive preference in the beginning as well as low number of successful flies obscures the results. Reason for the disturbed OR at the end is that the offset for one fly was accidentally shifted while attempting to restart flight with a brush.
Results from tethered flight in torque meter. Fly fixed to hook and flight torque measured while laser punishes either left or right turning. Higher PI score indicates longer stay in unpunished domain, orange colored periods are punished. Higher scores in period 9, 12 and 13 indicates learning. Fly genotype is Wild type Berlin, N=16.Same as above, with PI adjusted to factor in initial domain preference. A low P-score indicates that the result is statistically significant and the flies indeed learned.Pie charts indicate efficiency of experiment success. Blue wedge represents flies that completed trial, other colors represent flies that failed. Efficiency still low, using laser at 100% duty cycle often causes flies to stop flying unless directed at exactly the right position on their antennae.
Drosophila brains with FoxP-LexA/LexAop-RFP. Mouse anti mCherry was only anti-RFP available so anti-mouse NC82 could not be used. Andy/Ottavias brains/VNCs for comparison
Torque results 12.02.2026, learning is abolished and many of the flies died. Think improper feeding is to blame. Granulated sugar had become encrusted and possibly inaccessible to the flies, potentially resulting in starvation. In the future, I’ll resolve to use less sugar and to clean more frequently to prevent this kind of buildup.
Results for 10.02.2026, 24 flies, 7 completed trial. Main source of failure was flies stopping flight and refusing to resume. Had some difficulty getting flightless torque on the unpunished domain.
Results from 3.2. with the laser at the lowest intensityResults from 5.2. with the laser at an increased intensityResults from 6.2. with the laser at about 1/3 intensityCombined results from last weekBreakdown of results
