Local FoxP knock out

on Monday, November 16th, 2020 8:47 | by

GMR65A06
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Set-Up test

on Monday, November 9th, 2020 11:03 | by

Wtb
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New review of the shiming-setup

on Monday, October 26th, 2020 1:56 | by

Recent wtb test

There often seems to be a strong preference for a side. The laser was off.

Fly had a strong pref for left side, laser was off

Flies often seem to have a strong preferenc for a side. In the imiage below the top fly was punished for left torque and survived, the fly below was punished for the right torque side and died.

Trails with drift

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Testing of the repaired torque-meter

on Monday, October 12th, 2020 12:49 | by

I’ve started again to test the set-up after repairs. It does not seem to be perfectly stable ,with some drift in the positive direction. Most often, it seems that the amount of drifting would be fine for measuring.

Therefor I tried measuring some flies. A problem now is a very negative baseline, when the fly stops flying. Also the response for left and right seems not to be equal.

So now I’m trying to get the line close to 0, so that I have conclusive results if the set-up if usable at the current state.

2 examples of drift:

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General behavior of the MBON-2

on Monday, August 31st, 2020 12:54 | by

I wanted to expand and look into some general behavior of the mbon-2 flies. Mostly as a complement to the already existing data.

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Results of new repairs

on Monday, June 8th, 2020 1:40 | by

No laser, just fly trace
Standart experiment

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Making sense of dwelling data

on Tuesday, March 24th, 2020 12:54 | by

Mean or median?

UnpunishedPunished
1001
13
1,21
0,52
0,35
5
Mean182,4
Median1,12

A highly hypothetical scenario of how the distribution of dwelling times could be. Even if unrealistic, it still illustrates the problem of using median instead of mean.

Plotting the dwelling times as means

An example fly of how the dwelling times are distributed over the different periods.

Grouped analysis of dwelling times

Cumming estimation plot

Used the R package Dabestr to plot the estimation statistics. The effect size and 95% CIs are plotted a separate axes
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UASCas9;UASgFoxP X ElavG4;TubGal80

on Monday, March 2nd, 2020 1:33 | by

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Comparison FoxP and FoxP/+

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UASCas9;UASgFoxP X ElavG4;TubGal80

on Wednesday, February 19th, 2020 1:13 | by

Update on coditional FoxP manipulation. Either no manipulation at 18°c or switch to 30°c for adult flys.

Top: switch to 30°c for adult flys.
Bottom: no switch in temperatur
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