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Planned experiments

on Monday, December 21st, 2020 11:37 | by Simon Benmaamar

Category: Rover/Sitter, Uncategorized | No Comments

Crossing Schemes: Differential dominance of tBh allele

on Monday, December 14th, 2020 1:41 | by Sayani Banerjee

Category: Uncategorized | No Comments

FoxP knockout in adult flies

on Monday, December 14th, 2020 12:06 | by Andreas Ehweiner

Knockout of FoxP in adult Drosophila, using nsyb Gal4 gen-switch line 80699. Flies were kept for two days on RU486 food.

Category: flight, Foxp, Memory, Operant learning, operant self-learning, Uncategorized | No Comments

further work on pcdf6 cloning, second experiment on larvae locomotion

on Monday, December 7th, 2020 12:33 | by Sarah-Lynn Stratil

PCR pCDF6 Primer
-12.5 µl primer reverse/forward
-5 µl dNTPs
-2.5 µl Polymerase
-0.57 µl pCDF6 undigested
-50 µl Q5 buffer
-166.93 µl H2O
Agarose-gel electrophoresis
E.Z.N.A. Gel Extraction

pcr1: 153.3 ng/µl
pcr2: 115.7 ng/µl
pcr3: 174.4 ng/µl

pCDF6 NEBuilder Assembly Reaction
-pcr1: 4.85 ng -> 0.88 µl
-pcr2: 4.24 ng -> 1.5 µl
-pcr3: 4.87 ng -> 1.6 µl
-pCDF6 digested: 100 ng -> 2.8 µl
-H2O: 3.22 µl
-10 µl NEBuilder HiFi DNA Assembly Master Mix/Control
PCR
heat shock transformation of the construct into competent E.coli cells
-> plated on LB0+Amp plates
-> no colonies

control: ELAV-Gal4 x UAS-gRNA (n=23)
experimental: ELAV-Gal4 x UAS-Cas9-gRNA (n=23)

Category: Foxp, Uncategorized | No Comments

Local FoxP knock out

on Monday, November 16th, 2020 8:47 | by Andreas Ehweiner

Category: flight, Foxp, Operant learning, operant self-learning, Uncategorized | No Comments

New evaluation of the Rover/Sitter larvae

on Thursday, November 5th, 2020 12:34 | by Simon Benmaamar

Control Group 1 has a higher density of larvae compared to Control Group 2.

Percentage of Sitter and Rover larvae, who stayed on the first food patch after 20 minutes.

Course Evaluation

Category: Rover/Sitter, Uncategorized | No Comments

First evaluation of the Rover/Sitter larvae

on Monday, November 2nd, 2020 11:17 | by Simon Benmaamar

I have tested 514 larvae from the forR/forS Control Group and 480 larvae from the forR/forS Crowded Group. Here are the results:

Here you can see the first Control Group I have tested 2 weeks ago. In total 203 larvae were tested (ForS: 101, ForR 102). Rover larvae left the food patch moren often, however there is only a small difference between the Groups: 4,95 percent of the sitter larvae and 8,82 percent of the rover larvae left the first food patch after 20 minutes.

Here you can see the second Control Group, which had a lower density compared to the first Control Group. 311 larvae were tested (155 sitter, 156 rover). Again rover larvae left the food patch more often than sitter larvae, however there is only a small difference between the groups. 14,19 percent of the sitter larvae and 19,87 percent of the rover larvae left the first patch. Interestingly both groups seemed to leave the first food patch more often compared to the first control group with a little bit higher density.

Here you can see the entire ForS/ForR Control Group (256 sitter larvae, 258 rover larvae). 10,55 percent of the sitter larvae and 15,50 percent of the rover larvae left the first food patch after 20 minutes.

Here you can see the entire ForR/Fors Group under crowded conditions. 236 sitter larvae and 244 rover larvae were tested. 9,75 percent of the sitter larvae and 5,33 percent of the rover larvae left the food patch A after 20 minutes.

Here the entire rover/sitter Control Group and Crowded Group were compared. 13,04 percent of the rover and sitter larvae from the Control Group left the first food patch whereas only 7,50 percent of the entire larvae from the Crowded Group left the first patch. So at least there seems to be an effect of the forage competition/food quality on the foraging behaviour.

This effect seems to come nearly exclusively from the forR Group.

Category: Rover/Sitter, Uncategorized | No Comments

New review of the shiming-setup

on Monday, October 26th, 2020 1:56 | by Andreas Ehweiner

Recent wtb test

There often seems to be a strong preference for a side. The laser was off.

Fly had a strong pref for left side, laser was off

Flies often seem to have a strong preferenc for a side. In the imiage below the top fly was punished for left torque and survived, the fly below was punished for the right torque side and died.