These are the results of the SMAP for the TNTxWTB. I also have done a few for the c105;;c232xWTB but there is not much to say. I would say that the cleanest lines show a bigger slope, but prone to subjectiveness.

In addition, I have done some animations of the attractors that I have posted on slack because of size.

praktikumandmine

Here I attach the results in a pdf file from the students praktikum with an additional line I tested on my own meanwhile (Gr28bd and TrpA1 drivers together). They seem to work as a really good positive control btw, good for technique optimization.

For the students I tried out two different split drivers, the MB058B, which targets PPL1-a’2a2, and MB301B, which targets PAM-b2b’2. In addition the Gr5a driver, because it targets the “sugar” neurons. From the split drivers I wanted to see if I still get a validation from my initial model. MB301B seems to do quite what my model would predict but MB058B maybe not. Hopefully in a future screen I would be able to test many more and make a much more precise modelling.

In the last meeting Björn proposed to do correlations of cumulative increasing bins. He said to do that taking the zeroth point (last library point where prediction is still not done) and use it for having a potential 1 of correlation coefficient at the beginning. I could not do that because I didnt save the zeroth points, and this will be a bit tedious and confusing considering that many flies were tested and probably the order is not 100% known. Thus, I just did the bins skipping this zeroth point. After all, we should see something similar with this one. First two graphs: c105;;c232>TNT (first and second prediction point), second: WTBxTNT, third: WTBxc105;;c232.

WTBxTNT

WTBxc105;;c232.

Examples of how each of the flies look like. So they are basically cumulative bins with each single fly (each in different colour). Just to have a hint how does the singularity looks like.     Second thing I did is normalize the to have a range from -1 to 1 all of them (I have to double check the range in the script) and also setting them at a starting point of zero. I did this because we do not want to have differences in the correlation coefficient due to a different offset of the values of the wing beat and neither because of the starting point (if the fly was already flying to the right full gas, then it could be that it has an influence in the following prediction).

c105;;c232 –> first at starting at zero without normalizing and then with normalizing. The next is just the RMSE (not so important).

To see if there is an exponential decay in the prediction of the fly traces we did correlations of bins of 40 data points. We have 4 graphs (the last one merged) which consist of predictions at two different points with two different number of neighbours used for the prediction. So we have for each group sucesively: prediction at the first prediction point with the first number of neigbours, then the same with different number of neighbours. The last two are two different numbers of neighbours for the second prediction point. In the order: c105;;c232>TNT, TNTxWTB, c105;;c232xWTB

This is now the results from trying to predict the fly behavior doing ensembles of two predictions for the next 200 data points at two different points of the traces.

c105;;c232>TNT:

WTBxTNT:

WTBxc105;;c232:

From what we see here, there is no “flattening” in the prediction of the fly when the neurons under c105 and c232 are targeted by TNT. This is  done with around 14/15 flies for each group with two predictions in each ensemble of the two starting points. That makes a total of 15flies x 3 groups x 2 starting points for prediction x 2 predictions per ensemble = 180 prediction traces. Now I´m trying to calculate it by making correlations of bins in the prediction-observed for the same fly

linearmodelling

This is the markdown showing the protocol and results of the modelling for the choice in the T-maze. This is for calculating valence. Nevertheless, this needs to be confirmed with the results of more lines, it could be that it is overfitted, I would like to do in addition cross-validation. I´m actually doing crosses and finding new lines to have more lines to test.

This is the prediction analysis of flies in the platform under a 20min experiment under dark conditions. The number of experiments change drastically among groups because of technical problems: WTBxTNT is 4, WTBxc105;;c232 is 22, for the experimental line is 6 (c105;;c232>TNT), for the platform without flies is 10. I show the root mean squares and the correlation coefficient for each group.

This is the experimental group: c105;;c232>TNT.

The group without flies on the platform. I expect here to get a very good predictability overall:

WTBxc105;;c232 group WTBxUAS-TNT

These were done the week before last one but I could not upload it last time, so here are they. These are the results for predicting 4-5 flies of each group. Just one prediction from the middle of the time series for the 40 data points ahead in the future. First group is c105;;c232>TNT.

This is c105;;c232 x WTB

This is UAS-TNT x WTB

As I saw that the graph had so much zig-zag I told Pablo to make a bigger number of tested flies and this is what he is presenting today.

In addition I did analyze other parameters which are all saved under a PDF file below (Strokelitude). This contains some parameters with doubtfull processing which I still don´t trust so I have to find a better way for the calculation of it.

Strokelitude

These are just 5 flies (WTBxTNT) from the strokelitude where I measured the correlation coefficient on the Y-axis. In the X-axis, first bin is from 0-2 s of prediction, second is 2-4s and so on.

It seems as if some flies do nicer than others. Although it seems to me that a correlation coefficient from 0.3 isnt a big thing with all this variability. I have to find out the best binning though, I think it needs to be much more in the short term.

When I do the mean of the 5 flies measured, I do see a very slight decay. But once more I would say the decay is from the bin 1 to the second.

Here I tried another way, the RMSE, which according to literature and to my own reasoning should be a better analysis. I think RMSE measures just the differences of the absolute points whereas correlation coefficient is rather if the direction and degree of variation correlates (covariates). I find a very weird result. The fit is bad, the it gets better (but it should be just a chance event because correl coef decreases) and then it get very bad and so on.

I think for the future I have to make ensembles of two k neighbours maybe, which seem to increase the prediction power 10-15%. And maybe not look that much into the future as it was done here (10s).

Here some examples of predictions vs observations:

Spectrum with the x axis showing the frequency so that 0.5 is half of the measuring frequency (in this case 20Hz and so it measures until the Nyquist frequency: 10Hz).Here we see the result for the three platforms without any flies. From time to time I hit the table to make some signal in the platforms

To compare the power of the frequency put together platform with (green and black) and without flies (red). This is the case of three different flies in each platform.

Here the same flies, just in different platforms. There we see that some platforms show stronger signal than other (if I remember well). So is not the fly making the difference. But I have to check. Measuring in this case the 250Hz raw data. In black with a fly and in red without fly

Measuring in this case the 250Hz raw data. This time with and additional fly (green). We can see a characteristic peak at around 60Hz.