There had been some concerns about the optomotor display in the group evaluation sheets showing right-turning torque on the left side of the graph and vice versa. Also, the use of standard deviations seemed to blur differences between the experimental groups:

Because of these concerns, I have swapped the traces and used standard error of the means instead of standard deviations:

What do you think? Better or worse? Feedback very welcome!

The following picture shows the results for the conducted preference tests (pure/salt) for sNPF mutants. All Experiments were performed under red light.

Over the course, control and test lines were experimented with under ‘Joystick’. The test lines include 13:0273-Gal 4 line, SS56699 line, and TH-C-AD; TH-D-DBD line. The tests were done under red and yellow light conditions.

Red light conditions:

Yellow light conditions:

At long last, I got all the flies together that we need for sufficient statistical power. As the preliminary data had indicated, WTB flies don’t learn with the short training, while rut and rsh flies do just fine.

However, this may be due to genetic background effects, so we need to check the CRISPR mutants.

The flies without curly (straight wings) for the line NorpA;20xUAS-Chrimson were tested in T-maze in dark-light conditions. The tests were conducted on 23 November, 27th November, and 29th November 2023. 8 tests were done for each pink and green line; hence a total of 16 tests were conducted. A total of 273 flies and 233 flies were tested for pink line and green line respectively.

Last week, the torque mete ran for two days and I managed to record a few radish flies:

Finally, thanks to Marcella gluing to fly wheels instead of one, the mutant data are starting to roll in on the shortened self-learning experiment:

Short yaw torque learning, i.e., only one minute per period. Orange: training, yellow: test. WTB: wild type Berlin, rut: rutabaga learning mutants. With this short training of only 4 minutes, wild type flies show no torque preference in the after training, while at least the first few rutabaga flies show such a preference:

So far, I only could get one radish fly to make it through the experiment, so I cannot display it here.

Below you can find the data I collected from the offspring flies:

-> offspring from trained parents

-> offspring from untrained parents

(I left out data from flies that showed negative preference during two training periods in a row)