Last week I started to test CsChrimson (NorpA, 20xUAS-Chrimson) flies with the joystick set up, to reproduce Daniels’ data on the positive control and to get used to the experimental set up.

There is a noticeable improvement (from my end) from the first two days of testing to the last two days.

These are the results of the first four days of testing. All in all, the positive control shows good avoidance. Althoug, there is a remarkable change in the error bars starting with the fifth period. Maybe due to exhaustion or any other effect that I don’t know of yet.

I replicated the analysis of the single-cell RNA-sequencing data of the ventral nerve cord from Allen et al., (2020)

tSNE cluster map

rut, rad, VGlut, FoxP expression in clusters:

Aaron M Allen Megan C Neville Sebastian Birtles Vincent Croset Christoph Daniel Treiber Scott Waddell Stephen F Goodwin 
(2020) A single-cell transcriptomic atlas of the adult Drosophila ventral nerve cord
eLife 9:e54074.
https://doi.org/10.7554/eLife.54074

16 flies went through the test out of 48 flies

I retook the images of last week’s brains for MBON02-transtango (trans-tango mkII) imaging. Green channel shows MBON02, red channel shows post-synaptic cells of MBON02: