When Andi tested his aPKC CRISPR knock-outs, he used the Tang torque meter setup, where the OMRs aren’t recorded. So I’m attempting to replicate his results and compare OMRs between groups.

It looks like I need to tweak the graph in some way to make the density plots show up. Obviously, N is too low to say anything yet, but I need to work on the plot.

Below you find the data from my experimental rounds A and B:

-> learning scores of the parental flies from experimental round A and B

-> learning scores of the trained parent´s offspring only from experimental round A

-> learning scores of the untrained parent´s offspring only from experimental round A

The results confuse me a lot and I am happy to discuss reasons :) However the offspring of the round-B will be ready for testing by the end of this week so there is still some data to collect…

Via gDNA analysis and PCR was the specific area of the rsh gene extracted and amplified where the nucleotide substitution: C to T (Folkers et al., 2006) should be for the rsh¹ mutation. The amplicon was Sanger sequenced which proved the nucleotide substitution.

Knockout of Kibra in adult flies.

Knockout of Kibra during development.

New experiments added for yellow light, ATR and NOATR Control group, ATR Sifa-G4 and ATR 13.0273-Gal4.

These are the result of Joystick experiements of Control group with ATR and without ATR yellow and red light, SifaG4 group with ATR, yellow and red light, 13.0273Gal4 group with ATR, yellow and red light.