I wanted to expand and look into some general behavior of the mbon-2 flies. Mostly as a complement to the already existing data.

-Introduced Sayani to the wonderful world of Drosophila

-Been doing some DTS coding

-Preparing flies to do optomotor response for Mathias Raß

DTS coding
-Added progressbar for data validation
-Updated the progress bar (see figure 1)
-Fixed bug with wrong sample size (see figure 2)
-Fixed bug with unorganized barplots (see figure 2)

Exp always to the right:
plotOMparams <- plotOMparams[order(plotOMparams$desc),]
plotOMparams$group <- factor(plotOMparams$group, levels=paste(unique(plotOMparams$group)))

Samplesize fix:
samplesizes.annotate(boxes, as.numeric(table(plotOMparams$desc)))

Progressbar:
progress <- c(round(l(100/(length(xml_list)))),round(flycount(100/(totalflies))))

Rescreening:
-Finished rescreening last Thursday. Started to evaluate the new data

Optomotor platform: Ran a few more tests to confirm that the machine was still working, it is. I also adjusted the 0 line so that it is at 0, by readjusting the “zero line” screw. Looks much better now but it is still not perfectly at 0. A difference 0.1 on the computer screen translates to 100 in the evaluation chart.

Optomotor platform:
Ran a few more tests to confirm that the 0 line is always at 0. Readjusted the “zero line” screw. Looks much better now. It is still not perfectly at 0 but a difference of 0.1 in the chart translates to 100 in the evaluation graph.

Recently I measured the optomotor response in T4/T5 flies. As expected, they did not respond to the optomotor stimulus as seen in the left chart below. However, statistical evaluation struggles to quantify this difference. It might be that this is because of the low sample size, or that we are using the wrong statistical analysis?

The old platform works a lot more consistently compared to the new one. Unless major improvements are seen I do not see any reason to update it.

• Added a timer
• Previous version generated faulty xml files. This bug has been squashed (see image below)
• Display window of the software now also prints which direction the striped arena turns

A new platform have been designed for the optomotor response. It is placed on a pivot with the axis attached on the back. In contrast to the previous design witch only monitored movement in the left/right direction, the new design in theory allows the fly to rotate the platform.