FoxP-KO: the homozigous FoxP-KO (with NO Gal4 and NO GFP) had a green signal, it means that the dsRed gives problems. I thus removed it. Also, in really nice dissections, I don´t see the phenotype I was seeing before. The brain looks fairly normal. it could than be that i wasn´t disecting well enough

The following Link shows the results of the latest tests with Wildtype flys, evaluated with the latest version of the HTML_DTS_project.R programm.

https://drive.google.com/open?id=1xNYvqD-wSggNl-ENkA034vVybLiv3wGj

The histograms unfortunately show very little difference between the state of left turning arena and right turning arena.

MB057B

MB077B

This picture shows all of the positive clones in the ELISA from the 1st screen

While this picture shows just the clones that have a high response for the peptide the mice where injected with, a low response for any other random peptide, and furthermore a low response for BSA

I add another animal to the count (so now n=5) and the standard deviation increased. Since I used a very young fly it could be that the cell number is age dependent. But since it can be also individual differences, after San Diego I will make an age related analysis

Latest Tests:

D1

D2

D3

XML Constructor:

What else should be integrated?

Here´s  a picture of a WTB fly and a foxP-KO-homozigous. I dissected all together and I did not know which one was which. But I manage to recognize them, which means that they are well recognizable. Just to be sure i will do that another time. (Also in order to have better dissections).

The distance between the two “hemispheres” in the WTB has an average of 5,4 um (n=3)

The distance between the two “hemispheres” in the KO has an average of  32,2 um (n=2)

The length of the protocerebral bridge in the WTB has an average of 95,7 um (n=3)

The the protocerebral bridge in the KO has an average of  140,3 um (n=2)

These are the results of my last experiment in Regensburg! I wanted to see what happens when I inhibit the two interesting lines: TH-D1 for approach and TH-D’ for avoidance. Both seem to want to inhibit the neurons. Explanations??

Adult brain

Adult ventral nerve cord