further work on pcdf6 cloning, second experiment on larvae locomotion
on Monday, December 7th, 2020 12:33 | by Sarah-Lynn Stratil
PCR pCDF6 Primer
-12.5 µl primer reverse/forward
-5 µl dNTPs
-2.5 µl Polymerase
-0.57 µl pCDF6 undigested
-50 µl Q5 buffer
-166.93 µl H2O
Agarose-gel electrophoresis
E.Z.N.A. Gel Extraction
pCDF6 NEBuilder Assembly Reaction
-pcr1: 4.85 ng -> 0.88 µl
-pcr2: 4.24 ng -> 1.5 µl
-pcr3: 4.87 ng -> 1.6 µl
-pCDF6 digested: 100 ng -> 2.8 µl
-H2O: 3.22 µl
-10 µl NEBuilder HiFi DNA Assembly Master Mix/Control
PCR
heat shock transformation of the construct into competent E.coli cells
-> plated on LB0+Amp plates
-> no colonies

experimental: ELAV-Gal4 x UAS-Cas9-gRNA (n=23)
Category: Foxp, Uncategorized | No Comments
New experiments on Rover/Sitter larvae
on | by Simon Benmaamar
Testing new tracking software: Kinovea

Food patch experiments on old Rover/Sitter larvae
Distance tracking of old Rover/Sitter larvae
Category: Larve, Rover/Sitter | No Comments
Local FoxP knok out GMR20H5
on | by Andreas Ehweiner
Category: flight, Foxp, Operant learning, operant self-learning | No Comments
Distance tracking of larvae
on Monday, November 30th, 2020 11:57 | by Simon Benmaamar
Testing of the tracking and R Code

Distance tracking of Canton-S wild type larvae

Problem finding the video time

Category: Larve, Rover/Sitter | No Comments
Experiments on larvae locomotion using control and experimental line
on Thursday, November 26th, 2020 10:55 | by Sarah-Lynn Stratil
Category: Foxp | No Comments
PCR with undigested pCDF6
on Monday, November 23rd, 2020 12:54 | by Sarah-Lynn Stratil
primer: reverse/forward each 10 µl
dNTPs: 4 µl
buffer: 40 µl
polymerase: 2 µl
H2O: 133.6 µl
undigested vector: 0.4 µl

Category: Foxp | No Comments
Further experiments on larval locomotion using Canton-S wild type flies
on Sunday, November 22nd, 2020 10:33 | by Simon Benmaamar

Tracking of the larvae using Ctrax
Category: Larve | No Comments
Dprime and D1 were verified
on Monday, November 16th, 2020 11:14 | by Sarah-Lynn Stratil
Category: Anatomy | No Comments
Local FoxP knock out
on | by Andreas Ehweiner
Category: flight, Foxp, Operant learning, operant self-learning, Uncategorized | No Comments
Experiments on larval locomotion using Canton-S wild type flies
on Friday, November 13th, 2020 11:07 | by Simon Benmaamar
Category: Larve | No Comments