FoxP-KO

on Sunday, November 25th, 2018 3:46 | by

FoxP-KO: the homozigous FoxP-KO (with NO Gal4 and NO GFP) had a green signal, it means that the dsRed gives problems. I thus removed it. Also, in really nice dissections, I don´t see the phenotype I was seeing before. The brain looks fairly normal. it could than be that i wasn´t disecting well enough

 

 

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Results generated by HTML_DTS_project.net

on Monday, November 12th, 2018 2:14 | by

The following Link shows the results of the latest tests with Wildtype flys, evaluated with the latest version of the HTML_DTS_project.R programm.

https://drive.google.com/open?id=1xNYvqD-wSggNl-ENkA034vVybLiv3wGj

The histograms unfortunately show very little difference between the state of left turning arena and right turning arena.

 

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Control experiments for GFP expression of MBON lines

on Monday, October 29th, 2018 2:38 | by

MB057B

MB077B

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Hybridoma (Fusion) 1st screen

on Wednesday, October 24th, 2018 5:06 | by

This picture shows all of the positive clones in the ELISA from the 1st screen

While this picture shows just the clones that have a high response for the peptide the mice where injected with, a low response for any other random peptide, and furthermore a low response for BSA

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FoxPB adult cell count

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I add another animal to the count (so now n=5) and the standard deviation increased. Since I used a very young fly it could be that the cell number is age dependent. But since it can be also individual differences, after San Diego I will make an age related analysis

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most recent test run and xml integration

on Monday, October 22nd, 2018 10:10 | by

Latest Tests:

D1

D2

D3

 

XML Constructor:

What else should be integrated?

 

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FoxP-KO or WTB?

on Wednesday, October 17th, 2018 4:04 | by

Here´s  a picture of a WTB fly and a foxP-KO-homozigous. I dissected all together and I did not know which one was which. But I manage to recognize them, which means that they are well recognizable. Just to be sure i will do that another time. (Also in order to have better dissections).

The distance between the two “hemispheres” in the WTB has an average of 5,4 um (n=3)

The distance between the two “hemispheres” in the KO has an average of  32,2 um (n=2)

 

The length of the protocerebral bridge in the WTB has an average of 95,7 um (n=3)

The the protocerebral bridge in the KO has an average of  140,3 um (n=2)

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on Monday, October 15th, 2018 3:01 | by

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Inhibiting the interesting lines

on Monday, October 8th, 2018 2:44 | by

These are the results of my last experiment in Regensburg! I wanted to see what happens when I inhibit the two interesting lines: TH-D1 for approach and TH-D’ for avoidance. Both seem to want to inhibit the neurons. Explanations??

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17d-GFP with Bruchtpilot staining

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Adult brain

   

 

Adult ventral nerve cord

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