Thornlabs spectrometer working!
on Monday, November 20th, 2017 2:59 | by Christian Rohrsen
After getting a new spectrometer, we confirmed that the first one was faulty. Comparison of old (1st and 3rd measures) and new (2nd and 4th) spectrometer. Now much more sensitive and the right spectrum measured for the green LED present in the spectrometer itself and for the light comming out of the light guide coupled with the red LED (whose spectrum does not seem to change after travelling through the light guide)
Category: neuronal activation, Operant reinforcment, Optogenetics | No Comments
thf1 line from screen
on Monday, July 17th, 2017 2:40 | by Christian Rohrsen
This is the first line I completed from the screen. What it is more important is that I found out that NorpA;UAS-Chrimson has orange eyes, when it should be red. So I think I will start to do the line again and meanwhile test the contaminated line to see what the phenotype looks like
Category: neuronal activation, Operant reinforcment, Optogenetics, python code | No Comments
Looking for students
on Monday, June 19th, 2017 6:01 | by Christian Rohrsen
Category: neuronal activation, Operant reinforcment, Optogenetics | No Comments
Measuring locomotion in the Joystick? Something seems to happen…
on Monday, June 19th, 2017 2:39 | by Christian Rohrsen
Example of ON/OFF traces. And then the wiggling was measured in both conditions (with and without light). It seems like they move more when they are in the light side. I also did measure the derivative with a tau=2 (in case the sampling is over the temporal frequency of fly behavior)
Category: neuronal activation, Operant reinforcment, Optogenetics, python code | No Comments
Comparing T- and Y-maze
on Monday, June 12th, 2017 2:59 | by Christian Rohrsen
Comparing scores in both setups show some lines that match their scores and other lines that have opposite scores. This filters the ones that are context dependent to the ones that show a context independent reinforcement valence.
Category: neuronal activation, Operant reinforcment, Optogenetics | No Comments
Optimizing the Joystick with Gr28bd+TrpA1>Chrimson
on Monday, May 29th, 2017 12:58 | by Christian Rohrsen
It seems that there is something more there. There were 5 batches of 3 flies per side reinforced. I put the intensity higher than ever before. I will try to get the maximum intensity for the next experiment and see what it looks like.
Category: neuronal activation, Operant reinforcment, Optogenetics, python code | No Comments
Looking for the phenotype in Gr28bd+TrpA1>Chrimson
on Monday, May 22nd, 2017 3:01 | by Christian Rohrsen
I have decreased the light intensity to around half of what it previously was. The result do not show any clear phenotype. The ‘logical PI’ looks kind of inverted because I actually have plotted it so that positive means light, negative means no light. A total of 30 flies were tested: some of them with right, other with left reinforcement
Category: neuronal activation, Operant reinforcment, Optogenetics, python code | No Comments
Writting the analysis for the joystick experiments with Gr28bd+TrpA1>Chrimson
on Monday, April 24th, 2017 1:49 | by Christian Rohrsen
Example of a trace within the different trainning/test segments
Inter sampling interval just to check
10 experiment segments with alternating no reinforcement/reinforcement
The same as above but for the 3 platforms that ran in the same batch
Trace dynamics when the light is ON (blue) and OFF (red). Working on nicer visualization in general
Category: neuronal activation, Operant reinforcment, Optogenetics, python code | No Comments
Locomotion when light is on vs off
on Monday, February 27th, 2017 12:43 | by Christian Rohrsen
Category: neuronal activation, Operant reinforcment, Optogenetics | No Comments
Yoked experiments analysis
on Monday, February 20th, 2017 2:46 | by Christian Rohrsen
Category: neuronal activation, Operant reinforcment, Optogenetics | No Comments