aPKC knock-out

on Monday, May 17th, 2021 1:05 | by

aPKC was knock out using CRISPR Cas9 mediated editing.

PKC gRNA

on Monday, April 12th, 2021 11:07 | by

Comparison of the kock out of aPKC and PKC53e in adutl flies.

Problems with PKCi

on Monday, April 12th, 2021 10:55 | by

ElavGal4;tubGal80 X UASPKCi were raised at 18°C. Before the experiment they spent 4h at supposedly 35°C. Since they still show a stong learning phenotype the temperatur might have not been right.

PKCi

on Monday, March 22nd, 2021 12:35 | by

Test of 7 days old flies after GS

on Monday, February 15th, 2021 9:35 | by

Local FoxP knok out GMR11F02-GAL4

on Monday, February 15th, 2021 9:30 | by

Preliminary test of 14 days old flies after GS

on Monday, January 25th, 2021 1:51 | by

With RU468 (2d), GSnsyb

No RU, GSnsyb

FoxP knockout in adult flies

on Monday, December 14th, 2020 12:06 | by

Knockout of FoxP in adult Drosophila, using nsyb Gal4 gen-switch line 80699. Flies were kept for two days on RU486 food.

CD8GFP X nSyb-GS, 2d RU486

New review of the shiming-setup

on Monday, October 26th, 2020 1:56 | by

Recent wtb test

There often seems to be a strong preference for a side. The laser was off.

Fly had a strong pref for left side, laser was off

Flies often seem to have a strong preferenc for a side. In the imiage below the top fly was punished for left torque and survived, the fly below was punished for the right torque side and died.

Trails with drift

FoxP knock out in adult.

on Monday, September 14th, 2020 1:49 | by

The repetition of the knockout experiment in adult flies indicates that FoxP is not required for learning in adult flies. (Exp = elav tubGal80>Cas9gFoxP)