FoxP knockout in adult flies

on Monday, December 14th, 2020 12:06 | by

Knockout of FoxP in adult Drosophila, using nsyb Gal4 gen-switch line 80699. Flies were kept for two days on RU486 food.

CD8GFP X nSyb-GS, 2d RU486
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New review of the shiming-setup

on Monday, October 26th, 2020 1:56 | by

Recent wtb test

There often seems to be a strong preference for a side. The laser was off.

Fly had a strong pref for left side, laser was off

Flies often seem to have a strong preferenc for a side. In the imiage below the top fly was punished for left torque and survived, the fly below was punished for the right torque side and died.

Trails with drift

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FoxP knock out in adult.

on Monday, September 14th, 2020 1:49 | by

The repetition of the knockout experiment in adult flies indicates that FoxP is not required for learning in adult flies. (Exp = elav tubGal80>Cas9gFoxP)

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General behavior of the MBON-2

on Monday, August 31st, 2020 12:54 | by

I wanted to expand and look into some general behavior of the mbon-2 flies. Mostly as a complement to the already existing data.

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Progress Week 29

on Monday, July 20th, 2020 1:38 | by

-Introduced Sayani to the wonderful world of Drosophila

-Been doing some DTS coding

-Preparing flies to do optomotor response for Mathias Raß

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Results from rescreen

on Monday, May 4th, 2020 1:49 | by

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Some issues with UAS-TNT control

on Monday, April 20th, 2020 1:57 | by

I experienced some issues with the first batch of the UAS-TNT control flies, theyh ad a very low learning curve. The second batched looked fine but the still have a slightly lower learning PI during Test1 than I want.

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Making sense of dwelling data

on Tuesday, March 24th, 2020 12:54 | by

Mean or median?

UnpunishedPunished
1001
13
1,21
0,52
0,35
5
Mean182,4
Median1,12

A highly hypothetical scenario of how the distribution of dwelling times could be. Even if unrealistic, it still illustrates the problem of using median instead of mean.

Plotting the dwelling times as means

An example fly of how the dwelling times are distributed over the different periods.

Grouped analysis of dwelling times

Cumming estimation plot

Used the R package Dabestr to plot the estimation statistics. The effect size and 95% CIs are plotted a separate axes
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UASCas9;UASgFoxP X ElavG4;TubGal80

on Wednesday, February 19th, 2020 1:13 | by

Update on coditional FoxP manipulation. Either no manipulation at 18°c or switch to 30°c for adult flys.

Top: switch to 30°c for adult flys.
Bottom: no switch in temperatur
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Wtb Old vs Wtb New

on Thursday, January 23rd, 2020 1:21 | by

Comparison between the old and the new Wtb data.

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