Progress Week 29

on Monday, July 20th, 2020 1:38 | by

-Introduced Sayani to the wonderful world of Drosophila

-Been doing some DTS coding

-Preparing flies to do optomotor response for Mathias Raß

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Progress week 26-28

on Monday, July 13th, 2020 1:39 | by

Updates in DTS code

Refreshing dissection skills

Tdc2-Gal4 expression in adult brain
Actin-GFP expression in adult brain

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Progress for week 25:

on Monday, June 22nd, 2020 1:58 | by

DTS coding
-Added progressbar for data validation
-Updated the progress bar (see figure 1)
-Fixed bug with wrong sample size (see figure 2)
-Fixed bug with unorganized barplots (see figure 2)

Figure 1. Updated progress bar
Figure 2. Correct sample size and restructured barplot


Exp always to the right:
plotOMparams <- plotOMparams[order(plotOMparams$desc),]
plotOMparams$group <- factor(plotOMparams$group, levels=paste(unique(plotOMparams$group)))


Samplesize fix:
samplesizes.annotate(boxes, as.numeric(table(plotOMparams$desc)))

Progressbar:
progress <- c(round(l(100/(length(xml_list)))),round(flycount(100/(totalflies))))

Rescreening:
-Finished rescreening last Thursday. Started to evaluate the new data

Optomotor platform: Ran a few more tests to confirm that the machine was still working, it is. I also adjusted the 0 line so that it is at 0, by readjusting the “zero line” screw. Looks much better now but it is still not perfectly at 0. A difference 0.1 on the computer screen translates to 100 in the evaluation chart.

Optomotor platform:
Ran a few more tests to confirm that the 0 line is always at 0. Readjusted the “zero line” screw. Looks much better now. It is still not perfectly at 0 but a difference of 0.1 in the chart translates to 100 in the evaluation graph.

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Recent experiments

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Optogenetics rescreen final

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Final results of the optogenetics rescreen with Wilcoxon tests against zero and Bayes factors in the table
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Pupae TS increased N

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Category: buridan, Foxp | No Comments

Tdc2-Gal4 + FoxP-LexA in VNC

on Thursday, June 18th, 2020 4:16 | by

Red: FoxP
Green: Tdc2
No colocalization also in the VNC
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C380-increased N

on Wednesday, June 17th, 2020 9:49 | by

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Category: buridan, Foxp | No Comments

Cut off for drift

on Monday, June 15th, 2020 1:56 | by

What magnitude of drift is still ok?

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Last week

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  1. Crossed the flies for the course
  2. Almost finished the Buridan for C380
  3. Worked on the manuscript and supplemetary figures
  4. Done the FoxP reaction on induced embryo again because I was afraid after the different results Andi had last time
I fortunately still see nothing
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