FoxP knock out in adult.

on Monday, September 14th, 2020 1:49 | by

The repetition of the knockout experiment in adult flies indicates that FoxP is not required for learning in adult flies. (Exp = elav tubGal80>Cas9gFoxP)

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Test for hsGal4

Comparison between Control (elavGal4 x CD8GFP) and hsGla4 x CD8GFP. Hs for 3h, fixation and dissection after 24h.

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New figure?

on Tuesday, September 8th, 2020 11:16 | by

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Category: Foxp | No Comments

Side project

on Monday, August 31st, 2020 12:56 | by

Optomotor response
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General behavior of the MBON-2

on | by

I wanted to expand and look into some general behavior of the mbon-2 flies. Mostly as a complement to the already existing data.

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Ato x Cas9FoxP

Knockout of FoxP in Ato.

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PCB x Cas9gFoxP

Knockout of FoxP in the PCB. Flies are still able to learn.

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Test of 6 Gal4 lines

Test of the expression pattern of 6 Gal4 lines, while waiting for the cross to check for FoxP overlap.

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Category: Anatomy | No Comments

August

on Thursday, August 27th, 2020 1:46 | by

  • Halfway in analyzing the Buridan data altogether (not in two batches)
  • Maxiprepped plasmid for making FoxP protein at the klinikum
  • Writing the thesis a little bit
  • bought Fas-II antibody

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  • data 1): FoxP-iB Heterozygous/Homozygous comparison with Stinger-GFP. This time i was cautious with everything: fly all the same age and sex, same larvae density, same number of copies of GFP

I can not detect any difference between homozygous and heterozygous mutants. I also counted cells in IMARIS and I detected no difference in number or distribution.

  • data 2): FoxP-iB Heterozygous/Homozygous comparison with CD8-GFP. This time i was cautious with everything: fly all the same age and sex, same larvae density, same number of copies of GFP

I can see some differences but i do not know how to quantify/explain them. Also I am not sure if it is a problem of dissection/mounting. I am not convinced.

  • data 3): nc82 staining on homo/hetero FoxP-iB. I do not see differences

Problem: I haven’t managed yet to make the FasII antibody work:

I have used a 1:10 concentration of primary antibody for one night and a 1:100 concentration of secondary antibody for 4 hours. I will ask someone

In the picture I have increased the gain a lot just to be sure. I could not see anything

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Category: Foxp | No Comments

Progress Week 29

on Monday, July 20th, 2020 1:38 | by

-Introduced Sayani to the wonderful world of Drosophila

-Been doing some DTS coding

-Preparing flies to do optomotor response for Mathias Raß

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