update on self learning and longterm memory

on Monday, March 27th, 2017 2:07 | by

5 measurements on self learning from rover:

6 measurements on self learning form sitter:

10 measurements on self learning from wtb:

4 measrements on long term test from rover:

(right punishment)

3 measrements on long term test from sitter (fly stops a lot during test, this result may not be accurate):

(13:left punishment, 19: right punishment)

7 measrements on long term test from wtb

(left punishment)

 

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Behavioral Test on DA-GAL4s

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I am currently testing different dopaminergic GAL4s in order to validate previous results from my initial screening. Here I can find the results for one of those GAL4s

 

 

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TbH-LexAs and TDC2-Gal4 comparison

on Monday, March 20th, 2017 2:17 | by

I recently combined the two TbH-lexA  lines (54954 & 54075) with CD8GFP, and the TDC2-GAL4 line with CD8RFP, in order to compare their expression patterns. Here I present some of the dissections. The confocal is not working properly, but it is relatively good to draw some conclusions.

 

TDC2>GFP and anti-TβH (Scholz’s Lab)

TDC2>GFP (Gerber’s Lab)

anti-TDC2 (Goodwin’s Lab)

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PKG and WTB self learning test and long term memory test

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PKG rover self learning N=5

rover test on next day N=2

WTB self learning N=7

WTB test on next day N=4

2 rover, 4 wtb, 1 sitter test result after 24 hours

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DA neuronal populations and photopreference (counting neurons)

on Monday, March 13th, 2017 3:01 | by

After refining my DA screening, I end up having three interesting GAL4s which lead to changes in photopreference after expressing Shibire and rising the temperature. What I am trying to do now is to understand if they label the same neuronal population or not.

 

Genotype PAM PAL PPM1 PPM2 PPM3 PPM4 PPL1 PPL2 VUM
thF1>GFP 0 0 0,25 3,75 4,25 0 3 0,75 0
thF1;C’>GFP 0 0 1 7 5 0 2,5 5,5 1,5
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self learning from WTB

on Friday, March 10th, 2017 2:30 | by

By contrast of PKG rover, this result shows PI from wtb. N = 4

test1 PI= -0.05

test2 PI= -0.08

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PKG rover update

on Monday, March 6th, 2017 10:17 | by

There is the result of 20 measurements on PKG rover.

pretest:-0.45

train1:0.56

train2:0.71

test1:0.47

train3:0.83

train4:0.87

test2:0.39

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Locomotion when light is on vs off

on Monday, February 27th, 2017 12:43 | by

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PKG rover update

on Friday, February 24th, 2017 1:48 | by

After adjusting the laser angle, PI got much improved. But, the test 2 phase shows a lower PI than test 1 in most of cases, the reason for that is still unclear. The following is the result of 9 measurements without drifting.

train1 PI: 0.50

train2: 0.42

test1: 0.39

train3:0.61

train4: 0.63

test2: 0.06 ???

 

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Yoked experiments analysis

on Monday, February 20th, 2017 2:46 | by

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