Markers on Larvae

on Tuesday, April 23rd, 2019 11:18 | by

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Strokelitude – Final Work

on Monday, April 15th, 2019 2:41 | by

Final Stats (flew more than 8min/out of):
– Wild type: 14/17 (82%)
– ElavGal4 x UAS-Ryr 28919: 3/13 (23%)
– ElavGal4 x UAS-Ryr 65885: 13/100 (13%)

Wild Types:

ElavGal4 x UASRyR 65885

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anti-TH antibody on adults

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anti-TH antibody on larvae

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Differently from the adult, I did not find any colocalization between FoxPB and TH in larvae
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LexA construct ready?

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I just need to send for sequencing to verify

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Strokelitude – New Findings and Recordings (1.-7. Apr.)

on Monday, April 8th, 2019 1:14 | by

Last week (1.-7.Apr 2019) we noted how many flies want to fly more than 8 min and here is the result (only the ones we noted):
– Wild type: 8/9 (88%)
– ElavGal4 x UAS-Ryr 28919: 3/12 (25%)
– ElavGal4 x UAS-Ryr 65885: 6/35 (17%)

Trace (Downsampled 5) Graphs of Recordings

Wild type:

ElavGal4 x UAS-Ryr 28919:

ElavGal4 x UAS-Ryr 65885:

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WB using the guinea pig antibody

on Friday, April 5th, 2019 2:05 | by

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Continuation of the LexA contruct cloning

on Tuesday, April 2nd, 2019 2:14 | by

Digestion of the construct which has Gal4. Gal4 must be removed and exchanged with LexA
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Cas9 controls show a bit of genetic background

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Getting cyan in the projector

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The projector we are using is not an LCD projector. It is a DLP projector with a RGB LED light engine using a digital micromirror device to project the image. I used the GUI to access the LED driver control which allows you to project each LED individually or in combination. Forcing it into manual mode changed the way the projector displayed the colors. The projector takes in our case as input 24-bit RGB data at a frame rate of up to 120-Hz. The 120-Hz value is the upper limit of the projector and is only allowed using HDMI 2.1 cables. I am fairly sure that is not what I am using, but instead a standard HDMI allowing 60 Hz. This frame rate is composed of three colors (red, green, and blue) with each color equally divided in the frame rate. So using 120 Hz would allocate about 2.78 ms for each color, as we are using 60 Hz this number would be closer to 6 ms, the flickering I saw earlier is most likely a result of this.

However, accessing the manual mode of the LED driver control allows you to turn on the colors in pairs (or in 3 if using RGB). This got rid of the flickering

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