Update on DA & photopreference

on Monday, April 24th, 2017 1:55 | by

I am still testing different GAL4s in order to find out the dopamine clusters involved in photopreference shift. Here are some new results.

 

 

 

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Writting the analysis for the joystick experiments with Gr28bd+TrpA1>Chrimson

Example of a trace within the different trainning/test segments

Inter sampling interval just to check

10 experiment segments with alternating no reinforcement/reinforcement

The same as above but for the 3 platforms that ran in the same batch

Trace dynamics when the light is ON (blue) and OFF (red). Working on nicer visualization in general

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summary of self learning test data, and standardize data

on Monday, April 10th, 2017 1:19 | by

PKG-rover:

N = 24

pretest1 : -0.58

training1:0.53

training2:0.64

test1:0.22

training3:0.82

training4:0.84

test2:0.23

Standardization: ALL data +2?, then (test2-pretest)/pretest

standardized test2: 0.71

PKG-sitter:

N = 10

pretest1 : -0.24

training1:0.37

training2:0.58

test1:0.20

training3:0.75

training4:0.72

test2:0.41

standardized test2: 0.48

WTB:

N = 13

pretest1 : -0.46

training1:0.70

training2:0.73

test1:-0.01

training3:0.76

training4:0.81

test2:0.16

standardized test2: 0.57

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Photopreference shift and DA (new and old GAL4s)

on Monday, April 3rd, 2017 1:56 | by

Here I present new data on the GAL4s I have been testing. I also retested some previous ones. I had troubles with the shiTS/+ control, so the N has not chance since the last time.

 

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update on self learning&longterm memory

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longterm test:

1 from sitter,  8 from wtb, 3 from rover (total N = 12)

mean(test): 0.248          SD(test): 0.7

Longterm test Control

1 from sitter, 2 from rover (N=3)

mean(test):-0.386           SD(test):0.79

Two Sample:

Based on 50%(20%) of statistical power, the minimum sample size is 7 (17)for both group.

One Sample (assume control group has the average of PI is 0):

Based on 50% (20%) of statistical power, the minimum sample size is 22 (49)for both group.

https://www.dssresearch.com/KnowledgeCenter/toolkitcalculators/samplesizecalculators.aspx

 

Self learning test:

sitter N=5

wtb N= 4

 

 

 

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update on self learning and longterm memory

on Monday, March 27th, 2017 2:07 | by

5 measurements on self learning from rover:

6 measurements on self learning form sitter:

10 measurements on self learning from wtb:

4 measrements on long term test from rover:

(right punishment)

3 measrements on long term test from sitter (fly stops a lot during test, this result may not be accurate):

(13:left punishment, 19: right punishment)

7 measrements on long term test from wtb

(left punishment)

 

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Behavioral Test on DA-GAL4s

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I am currently testing different dopaminergic GAL4s in order to validate previous results from my initial screening. Here I can find the results for one of those GAL4s

 

 

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TbH-LexAs and TDC2-Gal4 comparison

on Monday, March 20th, 2017 2:17 | by

I recently combined the two TbH-lexA  lines (54954 & 54075) with CD8GFP, and the TDC2-GAL4 line with CD8RFP, in order to compare their expression patterns. Here I present some of the dissections. The confocal is not working properly, but it is relatively good to draw some conclusions.

 

TDC2>GFP and anti-TβH (Scholz’s Lab)

TDC2>GFP (Gerber’s Lab)

anti-TDC2 (Goodwin’s Lab)

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PKG and WTB self learning test and long term memory test

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PKG rover self learning N=5

rover test on next day N=2

WTB self learning N=7

WTB test on next day N=4

2 rover, 4 wtb, 1 sitter test result after 24 hours

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DA neuronal populations and photopreference (counting neurons)

on Monday, March 13th, 2017 3:01 | by

After refining my DA screening, I end up having three interesting GAL4s which lead to changes in photopreference after expressing Shibire and rising the temperature. What I am trying to do now is to understand if they label the same neuronal population or not.

 

Genotype PAM PAL PPM1 PPM2 PPM3 PPM4 PPL1 PPL2 VUM
thF1>GFP 0 0 0,25 3,75 4,25 0 3 0,75 0
thF1;C’>GFP 0 0 1 7 5 0 2,5 5,5 1,5
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