Test of 7 days old flies after GS

on Monday, February 15th, 2021 9:35 | by

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Local FoxP knok out GMR11F02-GAL4

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Larval food manipulation evaluation

on Monday, February 8th, 2021 1:28 | by

1. Food Patch

No significant difference between groups in Standard (wilcox.test, p =0.9442), Sugar (wilcox.test, p=0.3097) and Fat (wilcox.test, p-value = 0.06267).
Significant difference between Rover and Sitter in Standard_new (wilcox.test, p=0.01057), Carbohydrates (wilcox.test, p= 0.0002445), Starch (wilcox.test, p-value = 0.03431) and Yeast (wilcox.test, p = 0.001455)

2. Distance Tracking

Signifcant difference between Groups in Standard (t-test, p=0.0317), Sugar (t-test, p=0.0103), Fat (t-test, p=0.01823), Starch (t-test, p = 0.03895) and Yeast (t-test, p=0.001894).
No significant difference between Rover and Sitter in Standard_new (t-test, p =0.08108), Carbohydrates (t-test, p=0.09875),
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Larval food manipulation first evaluation

on Monday, February 1st, 2021 12:40 | by

Food Patch Experiment

The development of larvae that grew up on food without yeast was delayed by about 2 days. Larvae grown on starch feed developed faster by a day.
The standard food was probably too dry.

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Optogenetics- Joystick (THD’, THD1, Gr28bd-TrpA1)

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Preliminary test of 14 days old flies after GS

on Monday, January 25th, 2021 1:51 | by

With RU468 (2d), GSnsyb

No RU, GSnsyb

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Walking speed and stripe deviation: Buridan’s paradigm

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Power analysis and food manipulation recipes

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Power analysis sample size

Food manipulation recipes

Food recipes

Fly food protocol: 1 l of water was brought to a boil in a cooking pot using an induction cooker. Then 17 g of agar-agar dissolved in 350 ml of water was added and also brought to the boil. Diastatic malt extract and sugar beet sirup were added to the agar-water mixture. After that, polenta, brewer’s yeast and soy meal dissolved in 350 ml water were added to the pot. The mush was boiled for about 10 minutes while stirring continuously. Finally some water was added and after a short cooling down 6 g Nipagin dissolved in water was added. The mash was then filled into vials.

Nutrient distribution

a) Quantity distribution

b) Percentage distribution

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Rover/Sitter Density Experiment

on Monday, January 18th, 2021 1:30 | by

1. Food Patch Experiment

Control

There was a significant difference between the groups (t-test, p =0.01274)

Crowded

There was no significant difference between the groups (t-test, p = 0.2284)

2. Tracking

60 seconds

Control

There was a significant difference between the groups (t-test, p =0.000162)

Control

Crowded

There was no significant difference between the groups (t-test, p = 0.07565)

Crowded

45 seconds

Control

There was a significant difference between the groups (t-test, p =0.001252)

Control

Crowded

There was no significant difference between the groups (t-test, p = 0.2159)

Crowded

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GeneSwitch experiment

larval locomotion
dFoxP knockout in embryonic stage

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