To begin studying the sensory input related to photopreference shift after wing clipping, I decided to test some propioception mutants. Here are the first results.

Green: Intact flies

Yellow: Wing-clipped flies

TrpA1 self learning test, N= 47

TrpA1 long term memory, N= 39

Wtb long term memory (previous on fly simulator), N= 16

I am still trying to set up the optogenetics tools for my photopreference assay. These are the results so far. I combined the result obtained with Chrimson in the second chromosome and in the X chromosome, because they were identical.

Blue: only blue light.

Red: Blue light + pulsed red light.

I think Chrimson is already activated under my blue light condition.

N= 31

Videos of the GAD>Shi cross vs control

https://drive.google.com/file/d/0By0_Gagq_4TPN3kzMXRrT0VTX2s/view?usp=sharing

https://drive.google.com/file/d/0By0_Gagq_4TPUkRfZkM4amF1aDg/view?usp=sharing

This is something we talk last week. Here are the results for Speed and Stripe Deviation in the Buridan.

This is the first line I completed from the screen. What it is more important is that I found out that NorpA;UAS-Chrimson has orange eyes, when it should be red. So I think I will start to do the line again and meanwhile test the contaminated line to see what the phenotype looks like

30 from each lines, no statistially difference among them. I will keeping testing on flight simultor before transgenic flies hatch.