We need a student to finish up experiments

I am thinking of using optogenetics to study the neural substrates of photopreference. Here I present some controls that I have to do before starting. This is a T-Maze with blue light instead of with light.

These are the last controls I needed in order to be sure everything wrong was gone. They are not the best, but I think they show some clear results.

Yellow: Room Temperature

Green: 32°C

Example of ON/OFF traces.  And then the wiggling was measured in both conditions (with and without light). It seems like they move more when they are in the light side. I also did measure the derivative with a tau=2 (in case the sampling is over the temporal frequency of fly behavior)

N= 30, p-value = 0.66

Rescue from forS in visual memory defects:

adult-limit for expression in fan-shape body (c205-Gal80ts)

increase for expression in ellipsoid body (c819-Gal80ts)

N=20

Comparing scores in both setups show some lines that match their scores and other lines that have opposite scores. This filters the ones that are context dependent to the ones that show a context independent reinforcement valence.

To find the source of the problems I have seen in the T-Maze, I use lines from our stock and tested them in the T-Maze. This were reared at 25°C.

Rover,N= 36

Sitter, N= 28

WTB, N= 13

PKG affects K channels

Two ways to inhibitatePKG:

1

UAS-Gal4, RNAi

UAS-GCaMP3/OK6-GAL4; UAS-for RNAi/+

UAS-GCaMP3/OK6-GAL4; UAS-for RNAi/ UAS-for RNAi

2

PKG inhibitors:

1. KT 5823(10mM in DMSO)
2. (irrversible)

N=10