FoxP-KO or WTB?

on Wednesday, October 17th, 2018 4:04 | by

Here´s  a picture of a WTB fly and a foxP-KO-homozigous. I dissected all together and I did not know which one was which. But I manage to recognize them, which means that they are well recognizable. Just to be sure i will do that another time. (Also in order to have better dissections).

The distance between the two “hemispheres” in the WTB has an average of 5,4 um (n=3)

The distance between the two “hemispheres” in the KO has an average of  32,2 um (n=2)

 

The length of the protocerebral bridge in the WTB has an average of 95,7 um (n=3)

The the protocerebral bridge in the KO has an average of  140,3 um (n=2)

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on Monday, October 15th, 2018 3:01 | by

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Inhibiting the interesting lines

on Monday, October 8th, 2018 2:44 | by

These are the results of my last experiment in Regensburg! I wanted to see what happens when I inhibit the two interesting lines: TH-D1 for approach and TH-D’ for avoidance. Both seem to want to inhibit the neurons. Explanations??

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17d-GFP with Bruchtpilot staining

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Adult brain

   

 

Adult ventral nerve cord

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Signal to Noise Ratio, flight Performance (Het)

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Torque trace flies

https://drive.google.com/open?id=1utjuIrgQogT2lrRMl32VNdaBhsV_Ii5d

 

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Bits & Pretzels 2018

The fair took place from 30th September – 2nd Oktober (Sunday to Tuesday). I attended at Monday and Tuesday. The first two days were located at the Messegelände Ost Munich while for the last day the “Schottenhammel” Tent at the Oktoberfest had been reserved.

Monday:

Visit of the “Corporate Stage” which showcased Interviews:

Adidas

– The guest came from Adidias but worked independently first- before Adidas she used to make statistical interpretations on her own and present them to companies-> inefficient, it makes more sence to first inquire what the company is interested in- more and more Big Data with high tech factories- example of practical use of statistics: spike in sales as Kanye West fashioned a certain shoe

AI

– generally the new desired type of code is one that can handle uncertanty cases -> away from rule based coding, towards machine learning
– in germany data science is embraced very carefully as no direct value can be assigned to those tasks (in USA quite different)
– at the moment data tends to be scattered, investments have to be made to generate mass data
– should big conservative companies (eg Bosch) accecept help from software companies?
-> going for the long run through data science is a difficult topic in Ger because of the desire fropm investors to generate quick revenue and the strict data safety regulations
Linde Gase
– Linde as a rather conservative company, data science relatively new for them
– Where to go for innovative software design? Silicon Valley?
– Better alternative for Linde: Asia -> more producing Industry, cheaper, still good software developers
The code of the future
– presentation from a guy from stack overflow
– decoding techniques: 1. Rubber duckey debugging: tell a rubber duck on top of your screen what you did, what you want to do and what does not work (93% solved)
2. Divide and conquer debugging: break code up smaller and smaller until you reach the line with the problem
– minority problems in programmer community
– elitary high horse
– importance of algorithms for the public (influence of elections etc.) -> responsability lies with coders
Visit of “main stage”:
Digital revolution – What next?
– big startup investor Albert Wenger
– talk about the sense of income taxes (only ~20% of pupulation paying, majority of taxes comming from a even smaller number)
-> New big economic revolution, capitalism out of date? The battle is more about attention and less about money. Is a universal income necessary with advancing automatisation inevitable?
Emerging trends in Crypto
– How big of a role will Crypto Currency play in the Future?
Digital Competitiveness of nations. The urgency to rule digital innovation.
 
– Marc Walder (Ringier, Lobbyist for Digitalisation) & Phillip Rösler (former minister of economics in Germany)
– Switzerland having made the transistion to a digitalised country (place 5 on global scale)
– Germany having a long way to go (place 19 on global scale)
– long time consequences can be huge, economics will be more and more dependant on good digital infrastructure
– How to get there? Big companies have to invest and pressure the government (like in Switzerland)
The Innovative Ways Machine Learning is Disrupting Healthcare, Finance and Beyond.
– Tanmay Bakshi (machine learining expert etc.)
– Future of code lies in machine learning
– a lot of advertisement for his finacial management app
Tuesday:
 
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Program testing & Histogram Comparison

Is the program doing its job? Manual stimulation of the platform:

-> yes it is

 

Histograms

Why frequency histograms? I want to compare my data to the data from “Can a fly ride a bicycle?” (Wolf 1992):

example fly E7:

 

example E10:


 example E11

 

Histogram of 3 flys (periods of idividual flys have been merged -> one odd and one even period per fly):

Histogram of platform without fly:

Histogram of 11 flys (periods of idividual flys have been merged -> one odd and one even period per fly):

Histogram of 11 flys (no arenaturn):

Fly 3 (discussion model)

All Histograms of different tests for fly 3

-> merging makes little sence if the distribution is completely different areas for each fly!

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63x picture of 2 of the FoxPB-Gal4 positive clusters

on Sunday, October 7th, 2018 6:11 | by

The dorsal cluster neurons are really evident and with a typical organization

From Hassam et al., 2000

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Protocerebral bridge in FoxP-KO flies

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The more brains I see, the more I am convinced that the problem is not about my dissection

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MB in FoxPB-Gal4 with GFP on the 2nd chromosome (homozigous)

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this result is still to be verified by deleting the red fluorescent eyes to the flies

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