Looking for students

on Monday, June 19th, 2017 6:01 | by

We need a student to finish up experiments

Print Friendly

Starting with optogenetics in photopreference

on | by

I am thinking of using optogenetics to study the neural substrates of photopreference. Here I present some controls that I have to do before starting. This is a T-Maze with blue light instead of with light.

 

Print Friendly

Problems solved? (photopreference)

on | by

These are the last controls I needed in order to be sure everything wrong was gone. They are not the best, but I think they show some clear results.

Yellow: Room Temperature

Green: 32°C

 

Print Friendly

Measuring locomotion in the Joystick? Something seems to happen…

Example of ON/OFF traces.  And then the wiggling was measured in both conditions (with and without light). It seems like they move more when they are in the light side. I also did measure the derivative with a tau=2 (in case the sampling is over the temporal frequency of fly behavior)

Print Friendly

longterm memory test

on | by

N= 30, p-value = 0.66

 

Rescue from forS in visual memory defects:

adult-limit for expression in fan-shape body (c205-Gal80ts)

increase for expression in ellipsoid body (c819-Gal80ts)

 

Print Friendly

Update on Diazepam Experiement(15mM) and T-Maze Experiment

on Friday, June 16th, 2017 4:35 | by

N=20

 

Print Friendly

Comparing T- and Y-maze

on Monday, June 12th, 2017 2:59 | by

Comparing scores in both setups show some lines that match their scores and other lines that have opposite scores. This filters the ones that are context dependent to the ones that show a context independent reinforcement valence.

Print Friendly

Some controls for the photopreference Troubleshooting

on | by

To find the source of the problems I have seen in the T-Maze, I use lines from our stock and tested them in the T-Maze. This were reared at 25°C.

 

Print Friendly

R,S,WTB updates, and methods to inhibate PKG

on | by

Rover,N= 36

Sitter, N= 28

WTB, N= 13

PKG affects K channels

Two ways to inhibitatePKG:

1

UAS-Gal4, RNAi

UAS-GCaMP3/OK6-GAL4; UAS-for RNAi/+

UAS-GCaMP3/OK6-GAL4; UAS-for RNAi/ UAS-for RNAi

2

PKG inhibitors:

  1. KT 5823(10mM in DMSO)
  2. (irrversible)

 

 

Print Friendly

Diazepam Experiment (Conc= 15 mM)

on Saturday, June 10th, 2017 4:21 | by

N=10

 

Print Friendly