New FPGA software

on Monday, January 20th, 2020 2:02 | by

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Optomotor Response not really working

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  • Other projects I have been working on this week
  • Working out the new FPGA software for Flight simulator
  • Collecting flies for Optogenetics Experiment
  • Implementing dwelling times in DTS R script
  • Implementing Metadata comparison in R script
  • Grant writing
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Fist test of FoxP cond hs

First test of the conditionla FoxP hs with the heat applyed as adult.

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Testing of set-up

The focus of the laser was changed, resutling in a better avoidance.

Wtb1 = bevore readjustment, Wtb 2 after readjustment
Top: Cs with focused laser
Bottom: Cs with not adjusted laser
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CD8-GFP on Hom and Het on DCN

on Monday, January 13th, 2020 1:24 | by

Quiz: which is Het and which is Hom? Which differences can you see?

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Colocalization analysis

on Wednesday, January 8th, 2020 12:47 | by

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Crossings I did this week

on Tuesday, January 7th, 2020 11:57 | by

  1. Protocerebral bridge lines X CD8-GFP
  2. C380-Gal4 X UAS-gFOXP;UAS-Cas9 (+ 2 controls)
  3. stinger;Foxp-iB X FoxP-KO
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FoxP-iB_Het/Homo difference

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Updates on Optomotorics and DTS evaluation

on Monday, December 16th, 2019 1:57 | by

DTS: The graph below is an example image of how color according to category looks like. The output now adds a unique color for each category. The one below has three different colors as the pretest phase does not use any pattern and is therefor read as its own category.

Optomotorics: The software worked as it should although it had a couple of issues. Wrong information in xml sheet was one of them: this is now fixed so that it enters duration as seconds and the correct terms for a_pos and j_pos are used

The other issue was that at the end of the experiment about 40-50 data points were missing/removed. My speculation was that this either had to do with data being removed at the end of the experiment or that it ended prematurely. Turns out that the later was the cause.

As simple as it might seem, there was a lot of investigation to find this bug. To fix this I simply had to replace
if curr_count >= self.test_time() - 100:
with
if curr_count >= self.test_time() - 30:

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Het-Hom count finished

on Friday, December 13th, 2019 2:20 | by

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Category: Anatomy, Foxp | No Comments