on Tuesday, May 15th, 2018 12:26 | by Christian Rohrsen
Confocal image MAX stack of one of the brains at 20x
and at 40x
In this link we have a video of a 3D stainning pattern zoomed_CC
on Monday, May 14th, 2018 11:22 | by Christian Rohrsen
Axel stainning from both drivers together. I would say it really contains both driver lines.
This are my stainnings at the fluorescence microscope (no confocal). This is to show that in all of the 10-12 brains I have looked at, they all had the c232 pattern present
In addition, they had many more neurons outside from the central complex which I believe belong to the c105-G4 line. This is my only proof to show that c105 is also present, since the R1 neurons seem to be hidden when R2 neurons are stained.
on Monday, October 30th, 2017 1:32 | by Axel Gorostiza
Here I used the CaLexA tool with the elav driver, and reared the flies on constant darkness or under L-D cycles.
on Monday, October 9th, 2017 2:28 | by Axel Gorostiza
on Monday, March 20th, 2017 2:17 | by Axel Gorostiza
I recently combined the two TbH-lexA lines (54954 & 54075) with CD8GFP, and the TDC2-GAL4 line with CD8RFP, in order to compare their expression patterns. Here I present some of the dissections. The confocal is not working properly, but it is relatively good to draw some conclusions.
TDC2>GFP and anti-TβH (Scholz’s Lab)
TDC2>GFP (Gerber’s Lab)
anti-TDC2 (Goodwin’s Lab)
on Monday, March 13th, 2017 3:01 | by Axel Gorostiza
After refining my DA screening, I end up having three interesting GAL4s which lead to changes in photopreference after expressing Shibire and rising the temperature. What I am trying to do now is to understand if they label the same neuronal population or not.
on Monday, September 12th, 2016 1:37 | by Axel Gorostiza
In order to study the role of OA and DA in photopreference I am constantly looking for new drivers that label subpopulations of these groups. I recently found a TbH driver from the QF system, and wanted to know how representative of the TDC2-G4 neurons was. I have established a QUAS-mCherry;TbH-QFs line (Magenta) and crossed it with TDC2-G4;UAS-GFP (Green). In both cases, what is shown is the endogenous expression.
on Monday, February 1st, 2016 12:46 | by Axel Gorostiza
In previous experiments, I found one TβH(lexA)>shiTS combination that recapitulated TDC2>shiTS T-Maze results (http://lab.brembs.net/2015/09/looking-for-the-da-oa-neurons-involved-in-phototactic-flexibility/). Here I present the expression pattern of those two TβH-lexA drivers used.
TβH54075-LexA (Brain & VNC)
on Monday, December 7th, 2015 2:22 | by Axel Gorostiza
I am starting to study in more detail the genotypes that were positive in the DA screen. One was the TH-F1. Here I show the anatomy. I had several problems with the old confocal. I will try to use only the new one.
on Monday, November 23rd, 2015 2:29 | by Axel Gorostiza
In order to find which dopaminergic and octopaminergic neurons are related to light preference and the switch on it seen after clipping the wings, I decided to use a tool called CaLexA.
Here we can see my first attempt to use it. We can see the CNS from TH>CaLexA flies with and without wings.
I did not see any special signal in the brains, but I still have to play around a little bit more.