Homo and Hetero CD8-GFP;FoxPB-G4 Part2

on Monday, December 17th, 2018 11:56 | by

I kept analyzing brains and the phenotype is conserved

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Homo and Hetero CD8-GFP;FoxPB-G4

on Monday, December 10th, 2018 10:11 | by

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FoxP-KO

on Sunday, December 2nd, 2018 3:27 | by

FoxP-KO

WT (from random papers)

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Control experiments for GFP expression of MBON lines

on Monday, October 29th, 2018 2:38 | by

MB057B

MB077B

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17d-GFP with Bruchtpilot staining

on Monday, October 8th, 2018 2:32 | by

Adult brain

   

 

Adult ventral nerve cord

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63x picture of 2 of the FoxPB-Gal4 positive clusters

on Sunday, October 7th, 2018 6:11 | by

The dorsal cluster neurons are really evident and with a typical organization

From Hassam et al., 2000

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Protocerebral bridge in FoxP-KO flies

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The more brains I see, the more I am convinced that the problem is not about my dissection

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MB in FoxPB-Gal4 with GFP on the 2nd chromosome (homozigous)

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this result is still to be verified by deleting the red fluorescent eyes to the flies

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17d-GFP

on Monday, October 1st, 2018 2:37 | by

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17d

on Monday, September 10th, 2018 1:03 | by

Been working on 17d for the past month.

The experiments in the flight simulator. Self-learning performance indices in a two-minute test with the heat switched off after 4 and 8 minutes of training, indicated impairment of 17d-TNT flies.

The flies also showed clear impairments in their flight performance. To quantify this I assessed both possible alterations in their motor coordination (using climbing assay) as well as flight performance. The climbing assay relies on walking rather than flying. Both experiments show reduced ability of motor coordination and flight performance.

 

 

To confirm the specificity of the 17d-Gal4 fly I used the trans-tango flies.

Neuron, 96 (2017) 783-799. doi:10.1016/j.neuron.2017.10.011

 

The trans-tango is notorious for having a low expression in adult flies, which was also observed by me. The image is taking without any GFP-antibody.

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