TbH-LexAs and TDC2-Gal4 comparison

on Monday, March 20th, 2017 2:17 | by

I recently combined the two TbH-lexA  lines (54954 & 54075) with CD8GFP, and the TDC2-GAL4 line with CD8RFP, in order to compare their expression patterns. Here I present some of the dissections. The confocal is not working properly, but it is relatively good to draw some conclusions.

 

TDC2>GFP and anti-TβH (Scholz’s Lab)

TDC2>GFP (Gerber’s Lab)

anti-TDC2 (Goodwin’s Lab)

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DA neuronal populations and photopreference (counting neurons)

on Monday, March 13th, 2017 3:01 | by

After refining my DA screening, I end up having three interesting GAL4s which lead to changes in photopreference after expressing Shibire and rising the temperature. What I am trying to do now is to understand if they label the same neuronal population or not.

 

Genotype PAM PAL PPM1 PPM2 PPM3 PPM4 PPL1 PPL2 VUM
thF1>GFP 0 0 0,25 3,75 4,25 0 3 0,75 0
thF1;C’>GFP 0 0 1 7 5 0 2,5 5,5 1,5
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TbH QF system

on Monday, September 12th, 2016 1:37 | by

In order to study the role of OA and DA in photopreference I am constantly looking for new drivers that label subpopulations of these groups. I recently found a TbH driver from the QF system, and wanted to know how representative of the TDC2-G4 neurons was. I have established a QUAS-mCherry;TbH-QFs line (Magenta) and crossed it with TDC2-G4;UAS-GFP (Green). In both cases, what is shown is the endogenous expression.

tdc2-gfptbhqfs-cherrytbhqftdc2-merge-fixation-protocoltdc2-gfp-vnctbhqfs-cherry-vnctbhqftdc2-vnc-merge-fixation-protocol

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Octopaminergic neurons and phototactic flexibility

on Monday, February 1st, 2016 12:46 | by

In previous experiments, I found one TβH(lexA)>shiTS  combination that recapitulated TDC2>shiTS T-Maze results (http://lab.brembs.net/2015/09/looking-for-the-da-oa-neurons-involved-in-phototactic-flexibility/). Here I present the expression pattern of those two TβH-lexA drivers used.

TβH54954-LexA (Brain)

TbHlexA54954 2-1 (RGB)

MAX_TbHLexA.lif - TbHlexA54954 2-1

TβH54954-LexA (VNC)

TbHlexA54954 VNCs (RGB)

 

MAX_TbHLexA.lif - TbHlexA54954 VNCs-1

TβH54075-LexA (Brain & VNC)

MAX_TbHLexA.lif - TbHlexA54075 1 (RGB)

 

MAX_TbHLexA.lif - TbHlexA54075 1-1

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TH-F1 anatomy

on Monday, December 7th, 2015 2:22 | by

I am starting to study in more detail the genotypes that were positive in the DA screen. One was the TH-F1. Here I show the anatomy. I had several problems with the old confocal. I will try to use only the new one.

MAX_TH-F1nGFP4 MAX_TH-F1nGFP3.lsm (RGB) MAX_TH-F1nGFP2.lsm (RGB)MAX_TH-F1nGFPVNC2.lsm (RGB)MAX_TH-F1nGFPVNC1.lsm (RGB)MAX_TH-F1nGFPbrain1

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Phototactic flexibility – Neural substrates

on Monday, November 23rd, 2015 2:29 | by

In order to find which dopaminergic and octopaminergic neurons are related to light preference and the switch on it seen after clipping the wings, I decided to use a tool called CaLexA.

Here we can see my first attempt to use it. We can see the CNS from TH>CaLexA flies with and without wings.

I did not see any special signal in the brains, but I still have to play around a little bit more.

With Wings

TH CaLexA With Wings

Without Wings

TH CaLexA No wings C

TH CaLexA No wings B TH CaLexA No wings

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“Virtual brain” site

on Saturday, March 2nd, 2013 4:00 | by

Arnim Jenett (Janelia Farm Research Campus), Kazunori Shinomiya, Kei Ito (both Tokyo University), and other anatomists made a great site with a 3D-viewer of adult Drosophila brains available. You have the chance to scroll threw a whole mount stack while ticking different brain areas. Those brain areas are listed next to the stack. Different areas are coloured differently, so that you can look at the location of several areas in the same brain. On the main page you can find simply explained tutorials about the usage of the site. It is correlated to the anatomical search engine of the Janelia farm GAL4 collection.
Because it was very helpful to me to learn all the synonyms of relevant areas and because I think it is very helpful to learn more about the structure of the Drosophila brain in general I wanted to advertise the site here.
http://www.virtualflybrain.org/site/vfb_site/overview.htm

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