FoxP in the fan-shape body

on Friday, January 15th, 2021 12:10 | by

Control of general FoxP expression patter (line 104y)

Expression of 104y (green) and FoxP (red)

There seems to be no colocalisation of 104y and FoxP.

Expression of 121y (green) and FoxP (red)

Overlap of 121y GFP expression with FoxP expression is the fan-shape body.

Problems with line c205, no GFP expression.

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Dprime and D1 were verified

on Monday, November 16th, 2020 11:14 | by

Dprime
D1
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Gen-switch lines

on Monday, November 2nd, 2020 11:51 | by

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Gene switch finally worked

on Monday, September 28th, 2020 12:21 | by

I retried the gene-switch method with a new solution of RU486. Since it did not worked before I just tried one line. The flies were left for two days on instant food mixed with a sugar solution of RU486. This time I saw normal GFP expression. So I will test all 4 line in parallel after the break.

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Testing of possible Gal4-lines

I finished my cross for the colocalisation of FoxP with the 6 Gal4-lines i ordered.

Two lines show a nice colocalisation and will be tested.

One would show some overlap, but a completly diffrent expression pattern than it should have. So i will try this line again from the stock to exclude a mixup of the line.

The next one dont seem to have any overlap, but also the pattern looks not like it should.

This line seems to have coexpression but the pattern also looks a bit odd, this may be due to a general weak signal and a bad dissection.

The final line seems to have no overlap with the FoxP expression.

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Test for hsGal4

on Monday, September 14th, 2020 1:40 | by

Comparison between Control (elavGal4 x CD8GFP) and hsGla4 x CD8GFP. Hs for 3h, fixation and dissection after 24h.

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Test of 6 Gal4 lines

on Monday, August 31st, 2020 8:37 | by

Test of the expression pattern of 6 Gal4 lines, while waiting for the cross to check for FoxP overlap.

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Progress week 26-28

on Monday, July 13th, 2020 1:39 | by

Updates in DTS code

Refreshing dissection skills

Tdc2-Gal4 expression in adult brain
Actin-GFP expression in adult brain

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Potential Gal4-lines

on Monday, March 23rd, 2020 5:15 | by

Potential Gal4-lines matching parts of the FoxP expression.

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Search for neurons matching the FoxP expression pattern

I started looking at the FoxP expression pattern from Ottavia to see if i can identify the neurons.

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