Testing of possible Gal4-lines
I finished my cross for the colocalisation of FoxP with the 6 Gal4-lines i ordered.
Two lines show a nice colocalisation and will be tested.
One would show some overlap, but a completly diffrent expression pattern than it should have. So i will try this line again from the stock to exclude a mixup of the line.
The next one dont seem to have any overlap, but also the pattern looks not like it should.
This line seems to have coexpression but the pattern also looks a bit odd, this may be due to a general weak signal and a bad dissection.
The final line seems to have no overlap with the FoxP expression.
FoxP knock out in adult.
The repetition of the knockout experiment in adult flies indicates that FoxP is not required for learning in adult flies. (Exp = elav tubGal80>Cas9gFoxP)
Test for hsGal4
Comparison between Control (elavGal4 x CD8GFP) and hsGla4 x CD8GFP. Hs for 3h, fixation and dissection after 24h.
Ato x Cas9FoxP
Knockout of FoxP in Ato.
PCB x Cas9gFoxP
Knockout of FoxP in the PCB. Flies are still able to learn.
Test of 6 Gal4 lines
Test of the expression pattern of 6 Gal4 lines, while waiting for the cross to check for FoxP overlap.
Recent experiments
Cut off for drift
What magnitude of drift is still ok?
Results of new repairs
Test of set-up after repair.
The signal is drifting again. Also adding a spacer between clamp and device is not solving it, but making the signal more noisy.
