Cut off for drift
What magnitude of drift is still ok?
Results of new repairs
Test of set-up after repair.
The signal is drifting again. Also adding a spacer between clamp and device is not solving it, but making the signal more noisy.
New problems with the set-up
The measuring device needed repairing, after aperently some glued part got loosloosened and produced no signal anymore. The divice is now producing a signal again, but it is shifted.
The direction of the fly seems to be the main thing. Same fly faceing to the laser (left) and away (right)
Potential Gal4-lines
Potential Gal4-lines matching parts of the FoxP expression.
Search for neurons matching the FoxP expression pattern
I started looking at the FoxP expression pattern from Ottavia to see if i can identify the neurons.
Control cross
Control cross of UAS-Cas9;UAS-gFoxP with ELAV-GAL4;UAS-Cas9 is working. Flys are not able to fly and are walking poorly.
Control cross of Hs-Gal4 with UAS-Stinger is still not working.