3IY; N=11

Sucrose; N=10

Results show the gustatory preference for Canton S larvae under blue light. Positive PIs indicate a preference for the first named side of the petri dish e.g. positive PIs in Zucker-Pure group show larvae preferred the side of the sucrose substituted agarose gel.

Antibody Staining: FLP | GFP

Final Results

3IY; N=16

Sucrose; N=16

Buridan Test WTB; 5%Sucrose

Measurements in “Ping-Pong-Ball-Machine”

Measurements in combined Machines:

PPM2 flies showed light-avoidance in T-Maze experiments when tested in yellow light for 1 minute. In the JoyStick setting, where flies are tested for 10 one-minute periods flies displayed a shift from negative (light-avoidance) values in the beginning to no preference in the last training period. For this reason I started a set of T-maze experiments where flies could decide between light and dark tubes for 10 minutes.

In the red light 1-minute test setting flies did not show avoidance of light as strong as in the yellow light setting, but still tendencies for avoidance were observable. Therefore another set of 10-minute experiments will also be conducted with red light.

Update 03.02.25: Added more flies to the experiments and included some control flies.

Update 09.02.25: Added more flies; excluded all experiments where combined number of flies in light, dark and elevator tube was less than 30. Also added first red light results.

Update 14.02.25: Experiments with yellow light are almost finished, red light still needs some work

FLP; GFP ♂1

FLP;GFP♂2

FLP;GFP♀1

W|TH-Gal4-UAS-GFP|♂1

W|TH-Gal4-UAS-GFP|♂2

W|TH-Gal4-UAS-GFP|♀

13.0273 (Red)

For all Figures the left side displays all 10 testing and training periods for the JoyStick experiment. The right side compares the PIs of the last training period between the groups. Graphics indicate whether red or yellow light was used.

13.0273 (Yellow)

SIFa (Red)

SIFa (Yellow)