Control cross of UAS-Cas9;UAS-gFoxP with ELAV-GAL4;UAS-Cas9 is working. Flys are not able to fly and are walking poorly.

Control cross of Hs-Gal4 with UAS-Stinger is still not working.

Update on coditional FoxP manipulation. Either no manipulation at 18°c or switch to 30°c for adult flys.

First data of conditional FoxP temperature switch in adult flys

Proof of concept. I was playing around with ImageJ and found that there is a built in function already that does analyze the fluorescence intensity. I am just not sure exactly what it tells us. This is only as far as I have gotten yet.
The image below is the one I used.

The code generates the dwelling time for each period as well as the median dwelling time for each period. The code has also been implemented on grouped data but it only generates graphs for one of the groups, not both. Currently investigating why

I have been sorting and feeding flies ATR (in total about 7400 flies divided between 170 vials). Emer has been conducting the experiments during the previous week continuing this week.
Including the flies from today we have a total of 172 experiments divided between 7 experimental groups. Including the data from the Turkish students it is well over 230.

I looked at the control experiments and they look as expected. Perhaps even better than what the Turkish students had.

Slowly are we getting closer to getting a optomotor response
I made the following changes

• Glued flies to a needle rather than the plastic optic fiber
• Head of fly was not glued, the fly was able to rotate its head
• I put focus on making sure to get the fly as straight as possible
• Contrast between black and white was increased
• The light was adjusted to increase the brightness within the arena
  

• Other projects I have been working on this week
• Working out the new FPGA software for Flight simulator
• Collecting flies for Optogenetics Experiment
• Implementing dwelling times in DTS R script
• Implementing Metadata comparison in R script
• Grant writing

First test of the conditionla FoxP hs with the heat applyed as adult.