Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Parameters:
Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

Our experiments are performed after blinding ie we do not know the genotype of the flies tested. Code for this line is Yellow2. Intensity was approximately 400 Lux. The frequency of yellow light was 20Hz with 50:50 duty cycle.

Our experiments are performed after blinding ie we do not know the genotype of the flies tested. Code for this line is Red2. Intensity was approximately 400 Lux. The frequency of yellow light was 20Hz with 50:50 duty cycle.

Our experiments are performed after blinding ie we do not know the genotype of the flies tested. Code for this line is Orange3. Intensity was approximately 400 Lux. The frequency of yellow light was 20Hz with 50:50 duty cycle.

Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Light: intensity (500 Lux side, 1000 Lux bottom), frequency (20Hz)

The platform experiment was done with us being blinded. We referred to the genotype as the Red1 Genotype. The light intensity was taken as approximately 400 lux as was decided by previous experiments. The duty cycle was 50:50 at a frequency of 20 Hz.

Blue 1 combined PI value plot at 400 lux at 50 per cent duty cycle and 20 Hz

Trajectories of blue 1 flies on drosophila joystick apparatus shown:-

The behavior of new and old WTB seems different, but since only a few flies are tested, the results are not conclusive.

newWTB =3, PI= 0.35

old WTB from last month = 7,  PI=0.04

flying trace,

wtb72 (old, left punishment)

wtb 74,78 (new, right punishment)

PKG-rover:

N = 24

pretest1 : -0.58

training1:0.53

training2:0.64

test1:0.22

training3:0.82

training4:0.84

test2:0.23

Standardization: ALL data +2?, then (test2-pretest)/pretest

standardized test2: 0.71

PKG-sitter:

N = 10

pretest1 : -0.24

training1:0.37

training2:0.58

test1:0.20

training3:0.75

training4:0.72

test2:0.41

standardized test2: 0.48

WTB:

N = 13

pretest1 : -0.46

training1:0.70

training2:0.73

test1:-0.01

training3:0.76

training4:0.81

test2:0.16

standardized test2: 0.57

longterm test:

1 from sitter,  8 from wtb, 3 from rover (total N = 12)

mean(test): 0.248          SD(test): 0.7

Longterm test Control

1 from sitter, 2 from rover (N=3)

mean(test):-0.386           SD(test):0.79

Two Sample:

Based on 50%(20%) of statistical power, the minimum sample size is 7 (17)for both group.

One Sample (assume control group has the average of PI is 0):

Based on 50% (20%) of statistical power, the minimum sample size is 22 (49)for both group.

https://www.dssresearch.com/KnowledgeCenter/toolkitcalculators/samplesizecalculators.aspx

Self learning test:

sitter N=5

wtb N= 4