Below is a plot of all the flies of 11 genotypes for the wiggle difference. This is calculated by taking the sum of the difference of the tracepoint for each step. Thus, wiggle = sum(difference in tracepoint at each step). This is done for the entire 20 minutes time.

Now we wanted to measure the difference in on wiggle and off wiggle. On wiggle is the wiggle for when the fly was in the part which is supposed to have light on and similarly off wiggle is the wiggle when light is supposed to be off(that is in the portion in which we want to train it to be in). So below is the difference of on wiggle and off wiggle i.e – on wiggle – off wiggle:-

Now means of the difference in wiggle:-

Below is given the plot of effect-sizes of reinforcement of 11 genotypes. On the y-axis are the PI values for learning effect sizes.  These scores are calculated by taking the average of PI values of training periods and then subtracting pretest PI value from it. 

Yellow 1 (Positive Control): Gr28bd-G4, TrpA1-G4

Parameters:
Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

We wanted to measure learning and reinforcement in the flies for eight genotypes that we have tested as of now. For calculating the reinforcement scores we took the average of the two training periods combined PI scores and then subtract the PI value of the pretest period.

Reinforcement = average (combined training PI) – (combined pretest PI)

Below is the boxplot of the effect size for each fly for all these genotypes:-

We then calculated the mean of these reinforcement values for each of the screens and below is a bargraph for these means:-

Our experiments are performed after blinding ie we do not know the genotype of the flies tested. Code for this line is Orange1. Intensity was approximately 400 Lux. The frequency of yellow light was 20Hz with 50:50 duty cycle.

Our experiments are performed after blinding ie we do not know the genotype of the flies tested. Code for this line is Darkpink4. Intensity was approximately 400 Lux. The frequency of yellow light was 20Hz with 50:50 duty cycle.

Our experiments are performed after blinding ie we do not know the genotype of the flies tested. Code for this line is Darkpink3. Intensity was approximately 400 Lux. The frequency of yellow light was 20Hz with 50:50 duty cycle.

Our experiments are performed after blinding ie we do not know the genotype of the flies tested. Code for this line is Darkpink2. Intensity was approximately 400 Lux. The frequency of yellow light was 20Hz with 50:50 duty cycle.

Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Parameters:
Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4