Experimental Group: mb247-Gal4>UAS-ChR2-XXL, Control 1: mb247-Gal4>w1118, Control 2: w1118>UAS-ChR2-XXL, Tests are performed blind, 1.5M Salt

Experimental Group: mb247-Gal4>UAS-ChR2-XXL, Control 1: mb247-Gal4>w1118, Control 2: w1118>UAS-ChR2-XXL, Tests are performed blind

As N1-8 were perfomed within one week and differences in the size/developmental stage of the larvae within the same crossings/genotype in different vials were observed, the results of the measurements from the first week (N1-8) and of the measurements of the second week (N9-12) are analyzed separately and were additionally pooled.

Experimental Group: mb247-Gal4>UAS-ChR2-XXL, Control 1: mb247-Gal4>w1118, Control 2: w1118>UAS-ChR2-XXL, Tests are performed blind

Experimental Group: 201y-Gal4>UAS-ChR2-XXL (B), Control 1: 201y-Gal4>w1118 (C), Control 2: w1118>UAS-ChR2-XXL (A), Tests are performed blind

Experimental Group: 201y-Gal4>UAS-ChR2-XXL, Control 1: 201y-Gal4>w1118, Control 2: w1118>UAS-ChR2-XXL, Tests are performed blind

3rd instar larvae were tested for their avoidance behavior on 1.5% Agarose/1.5% Agarose+1.5M Salt-Plate. MB Kenyon cells were optogenetically activated via Channelrhodopsin2-XXL during the experiment. TH-Gal4 positive dopaminergic cells were permanently ablated.

MB032B>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

MB304B>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil

MB299B>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil