Dprime and D1 were verified
on Monday, November 16th, 2020 11:14 | by Sarah-Lynn Stratil
Category: Anatomy | No Comments
Gen-switch lines
on Monday, November 2nd, 2020 11:51 | by Andreas Ehweiner
Category: Anatomy | No Comments
Gene switch finally worked
on Monday, September 28th, 2020 12:21 | by Andreas Ehweiner
I retried the gene-switch method with a new solution of RU486. Since it did not worked before I just tried one line. The flies were left for two days on instant food mixed with a sugar solution of RU486. This time I saw normal GFP expression. So I will test all 4 line in parallel after the break.
![](https://lab.brembs.net/wp/wp-content/uploads/2020/09/Gen-switch-CD8GFP-1024x768.jpg)
Category: Anatomy, crosses | No Comments
Testing of possible Gal4-lines
on Monday, September 28th, 2020 12:11 | by Andreas Ehweiner
I finished my cross for the colocalisation of FoxP with the 6 Gal4-lines i ordered.
Two lines show a nice colocalisation and will be tested.
![](https://lab.brembs.net/wp/wp-content/uploads/2020/09/Overlapping-lines.jpg)
One would show some overlap, but a completly diffrent expression pattern than it should have. So i will try this line again from the stock to exclude a mixup of the line.
![](https://lab.brembs.net/wp/wp-content/uploads/2020/09/Error-line-2.jpg)
The next one dont seem to have any overlap, but also the pattern looks not like it should.
![](https://lab.brembs.net/wp/wp-content/uploads/2020/09/Error-line-1.jpg)
This line seems to have coexpression but the pattern also looks a bit odd, this may be due to a general weak signal and a bad dissection.
![](https://lab.brembs.net/wp/wp-content/uploads/2020/09/Fraglich-mit-overlap.jpg)
The final line seems to have no overlap with the FoxP expression.
![](https://lab.brembs.net/wp/wp-content/uploads/2020/09/No-overlap.jpg)
Category: Anatomy, crosses, Foxp | No Comments
Test for hsGal4
on Monday, September 14th, 2020 1:40 | by Andreas Ehweiner
Comparison between Control (elavGal4 x CD8GFP) and hsGla4 x CD8GFP. Hs for 3h, fixation and dissection after 24h.
![](https://lab.brembs.net/wp/wp-content/uploads/2020/09/Hs-vergleich-14.9.jpg)
Category: Anatomy, crosses | No Comments
Test of 6 Gal4 lines
on Monday, August 31st, 2020 8:37 | by Andreas Ehweiner
Test of the expression pattern of 6 Gal4 lines, while waiting for the cross to check for FoxP overlap.
![](https://lab.brembs.net/wp/wp-content/uploads/2020/08/Gfp-lines-1.jpg)
Category: Anatomy | No Comments
Progress week 26-28
on Monday, July 13th, 2020 1:39 | by Anders Eriksson
Updates in DTS code
![](https://lab.brembs.net/wp/wp-content/uploads/2020/07/Untitled-6-819x1024.jpg)
Refreshing dissection skills
![](https://lab.brembs.net/wp/wp-content/uploads/2020/07/Untitled-5-2.jpg)
![](https://lab.brembs.net/wp/wp-content/uploads/2020/07/Untitled-7.jpg)
Category: Anatomy, genetics, Lab, neuronal activation, personal, R code, science, Uncategorized | No Comments
Potential Gal4-lines
on Monday, March 23rd, 2020 5:15 | by Andreas Ehweiner
Category: Anatomy, Foxp | No Comments
Search for neurons matching the FoxP expression pattern
on Monday, March 23rd, 2020 11:07 | by Andreas Ehweiner
Category: Anatomy, Foxp | No Comments
Het-Homo OL
on Friday, January 24th, 2020 12:35 | by Ottavia Palazzo
Category: Anatomy, Foxp | No Comments