Testing of possible Gal4-lines
on Monday, September 28th, 2020 12:11 | by Andreas Ehweiner
I finished my cross for the colocalisation of FoxP with the 6 Gal4-lines i ordered.
Two lines show a nice colocalisation and will be tested.
![](https://lab.brembs.net/wp/wp-content/uploads/2020/09/Overlapping-lines.jpg)
One would show some overlap, but a completly diffrent expression pattern than it should have. So i will try this line again from the stock to exclude a mixup of the line.
![](https://lab.brembs.net/wp/wp-content/uploads/2020/09/Error-line-2.jpg)
The next one dont seem to have any overlap, but also the pattern looks not like it should.
![](https://lab.brembs.net/wp/wp-content/uploads/2020/09/Error-line-1.jpg)
This line seems to have coexpression but the pattern also looks a bit odd, this may be due to a general weak signal and a bad dissection.
![](https://lab.brembs.net/wp/wp-content/uploads/2020/09/Fraglich-mit-overlap.jpg)
The final line seems to have no overlap with the FoxP expression.
![](https://lab.brembs.net/wp/wp-content/uploads/2020/09/No-overlap.jpg)
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