Torquemeter Test and Confocal images of MBON02
16 flies went through the test out of 48 flies
I retook the images of last week’s brains for MBON02-transtango (trans-tango mkII) imaging. Green channel shows MBON02, red channel shows post-synaptic cells of MBON02:
MBON02 Trans-tango images
MBON02 trans-tango image from Kaun Lab. Left image shows trans-tango signal in red, right image shows overlay of all channels (green for MBON02, red for trans-tango, blue for nc82):
My MBON02 trans-tango images stained with anti-HA instead of anti-RFP. Flies generated by crossing trans-tango mkII line (BDSC: 95317) with MB399B (Brembslab stock number: 319). Left shows trans-tango signal in green, right shows overlay of al channels (green for trans-tango, blue for MBON02, red for nc82):
Janelia Flylight image of MB399B with 20XUAS-CsChrimson-mVenus trafficked in attP18 reporter:
Confocal Images Trans-Tango, Retro-Tango
Staining done by anti-GFP (MBON02), anti-RFP (post-synaptic cells), nc82 (for background stainingm not shown here). MBON02 cells are clearly visible on green channel but there is no clear cell signal on red channel (MBON02 in green channel, postsynaptic cells in red channel).
Here is the trans-tango image of MBON02 (postsynaptic cells of MBON02) from Kaun Lab:
Retro-Tango of b3 mns look worse, nearly no cell signal both in green (b3 mn) and red channels (b3 mn presynaptic neurons)
