Blocking with tetoxE
tetoxE was crossed to different Gal4-lines. 20A02 was tested separately before, 52B10, 55G08 and 64H04 were tested in parallel.
PB expression: 52B10, 55G08
EB expression: 20A02, 64H04
Previous experiment (Posted 30.08.21):
New test
![](https://lab.brembs.net/wp/wp-content/uploads/2021/12/tetoxEnew-666x1024.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/12/tetoxEnewstats.png)
EB: 64H04
aPKCdelta adult expression half the experiment time, increased sample size
Expression of aPKCdelta in all neurons in the adult fly (nysbGS-Gal4) for half the experiment time (1 min periods).
![](https://lab.brembs.net/wp/wp-content/uploads/2021/12/aPKCdeltaAdultHalf-3-1024x788.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/12/aPKCdeltaAdultHalfStats-1.png)
Pooling of both experimental periods PIs (P12, P13)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/12/aPKCdeltaAdultHalfStatsPool-3.png)
aPKCdelta adult expression half the experiment time
Expression of aPKCdelta in all neurons in the adult fly (nysbGS-Gal4) for half the experiment time (1 min periods).
![](https://lab.brembs.net/wp/wp-content/uploads/2021/12/aPKCdeltaAdultHalf-1024x788.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/12/aPKCdeltaAdultHalfStats.png)
aPKCdelta half the experiment time
Expression of aPKCdelta in FoxPiB positiv or motor neurons, the time of the experiment is half of the normal time.
![](https://lab.brembs.net/wp/wp-content/uploads/2021/11/apkcDelta-half-1-887x1024.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/11/apkcDelta-half-stats.png)
Colocalization of FoxP
Cross of FoxPLexA (red) with diffrent GFP-Lines (green)
GMR65A06
![](https://lab.brembs.net/wp/wp-content/uploads/2021/08/39330.65H05GfpFoxPLexA.jpg)
GMR20H05
![](https://lab.brembs.net/wp/wp-content/uploads/2021/08/47896.20H05Gal4FoxPLexA2-1-1024x1024.jpg)
GMR11F02 (dose not overlap)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/08/49828.11F02.GfpFoxPLexA2-1.jpg)
GMR52B10 not checked yet
![](https://lab.brembs.net/wp/wp-content/uploads/2021/08/38820.52B10GfpFoxPLexA.jpg)
aPKC (must be repeated)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/08/aPKC1-1024x1024.jpg)
GMR52B10 (PCB) block
Blocking of the line GMR52B10 (PCB, females) with males of Tetox (28838) or Kir (6595).
![](https://lab.brembs.net/wp/wp-content/uploads/2021/07/GMR52B10-block-887x1024.png)
![](https://lab.brembs.net/wp/wp-content/uploads/2021/07/GMR52B10-block-stats-1024x849.png)