Andreas Ehweiner

Blocking with tetoxE

tetoxE was crossed to different Gal4-lines. 20A02 was tested separately before, 52B10, 55G08 and 64H04 were tested in parallel.

PB expression: 52B10, 55G08

EB expression: 20A02, 64H04

Previous experiment (Posted 30.08.21):

New test

aPKCdelta adult expression half the experiment time, increased sample size

Expression of aPKCdelta in all neurons in the adult fly (nysbGS-Gal4) for half the experiment time (1 min periods).

Pooling of both experimental periods PIs (P12, P13)

aPKCdelta adult expression

Expressing aPKCdelta in all neurons in the adult fly.

aPKCdelta adult expression half the experiment time

Expression of aPKCdelta in all neurons in the adult fly (nysbGS-Gal4) for half the experiment time (1 min periods).

aPKCdelta half the experiment time

Expression of aPKCdelta in FoxPiB positiv or motor neurons, the time of the experiment is half of the normal time.

aPKCdelta expression

Expression of aPKCdelta, in FoxPiB positiv neurons or motor neurons.

Colocalization of FoxP

Cross of FoxPLexA (red) with diffrent GFP-Lines (green)

GMR65A06

GMR20H05

GMR11F02 (dose not overlap)

GMR52B10 not checked yet

aPKC (must be repeated)

Frist results retesting with tetoxE

Retesting of previously tested lines with other Tetox.

GMR20A02 block tetoxE

Blocking of the line GMR20A02 (females) with males of TetoxE or Kir (6595).

GMR52B10 (PCB) block

Blocking of the line GMR52B10 (PCB, females) with males of Tetox (28838) or Kir (6595).