Update: Looking for the DA & OA neurons involved in phototactic flexibility

on Wednesday, October 7th, 2015 11:35 | by

These are the newst results of my screen. There are some interesting candidates, but it’s still too soon to conclued something.


Update of the DA screening. New experiments are in red (25°C) and blue (32°C).



Experiment finished.


Looking for the DA & OA neurons involved in phototactic flexibility

on Monday, September 21st, 2015 2:41 | by

After using TH-G4 and TDC2-G4 to prove that dopamine and octopamine are involved in phototactic switch after wing clipping, I decided to find which neurons in particular are playing this particular role. Therefore, I started a few small screens with the GAL4s and LexAs available for these groups of neurons. I crossed the drivers to shibire to assess their role. For every graph blue means RT, and red means 32°C.

TH subsets-GAL4


Dopaminergic neurons -GAL4

Da clusters



New experiments: transparent T-maze

on Wednesday, April 3rd, 2013 9:14 | by

I have started doing experiments with the transparent T-maze, but I have some problems. The results are really weird: I have obtained negative phototaxis for both flies with intact and clipped wings (phototaxis is more negative for flies with intact wings????).

I did only 9 experiments, because I don’t really know how to run them and I need advice. I am going to explain how I was doing this experiments until now:

– First of all, I put the flies in the entrance tube of the T-maze (upper part) and I cover the T-maze with a black clothing (I want the flies to be adapted to the darkness, like in my last experiments with the opaque T-maze). They are in this situation for 10min.

– After that I push the flies into the elevator. I don’t know if I should let them stay there for 1 minute (like I was doing before with the opaque T-maze, or push them to the bottom of the T-maze to let them make their decision between darkness or light without an adaptation time.

I think that if they stay in the elevator for 1 minute, they will be again under light conditions, and that’s not good for the experiment. I could cover again the T-maze with the black clothing during this minute (that’s what I was doing until now, but with bad results).

– Then I let the flies decide for 30 seconds as always.

So if you have any suggestions, I will be glad to hear them :)



2nd Experiment

on Thursday, November 15th, 2012 2:39 | by

Yesterday, we cut the wings of half of the experimental flies.

We let them get over for 2 hours, and after that I repeated the same experiment we did last day, letting them choose between darkness and light.

There wasn’t a big difference between flies with wings and flies without wings, but the experiment shows flies without wings have less light preference than the other ones.