Here I used the CaLexA tool with the elav driver, and reared the flies on constant darkness or under L-D cycles.

L-D Cycle

D-D

Here I tested two common OA drivers in photopreference. Although the results are not conclusive, there could be something there.

Yellow: 25°C

Green: 32°C

This is a new try to set up the CaLexA technique.

TH-CaLexA L-D 12:12 Without Wings

TH: Magenta

GFP: Green

To begin studying the sensory input related to photopreference shift after wing clipping, I decided to test some propioception mutants. Here are the first results.

Green: Intact flies

Yellow: Wing-clipped flies

I am still trying to set up the optogenetics tools for my photopreference assay. These are the results so far. I combined the result obtained with Chrimson in the second chromosome and in the X chromosome, because they were identical.

Blue: only blue light.

Red: Blue light + pulsed red light.

I think Chrimson is already activated under my blue light condition.

This is something we talk last week. Here are the results for Speed and Stripe Deviation in the Buridan.

On this opportunity, I changed the size of the tubes in order to see if that has a impact on the proportion of flies that ended in the elevator.

Here, I present you the results of using Blue light for photopreference and adding a pulsed Red light. These experiments are controls required for using Optogenetics to study Photopreference. I used the Blue light at different intensities, and calculated the Choice Index (CI) and the proportion of flies in each tube of the T-Maze (White: Bright, Black: Dark, Grey: Elevator).